The cargo and the transport system

Secreted proteins and protein secretion in Trichoderma reesei (Hypocrea jecorina)

Markku Saloheimo (Corresponding Author), Tiina Pakula

Research output: Contribution to journalReview ArticleScientificpeer-review

67 Citations (Scopus)

Abstract

Trichoderma reesei (Hypocrea jecorina) is an efficient cell factory for protein production that is exploited by the enzyme industry. Yields of over 100 g secreted protein l−1 from industrial fermentations have been reported. In this review we discuss the spectrum of proteins secreted by T. reesei and the studies carried out on its protein secretion system. The major enzymes secreted by T. reesei under production conditions are those degrading plant polysaccharides, the most dominant ones being the major cellulases, as demonstrated by the 2D gel analysis of the secretome. According to genome analysis, T. reesei has fewer genes encoding enzymes involved in plant biomass degradation compared with other fungi with sequenced genomes. We also discuss other T. reesei secreted enzymes and proteins that have been studied, such as proteases, laccase, tyrosinase and hydrophobins. Investigation of the T. reesei secretion pathway has included molecular characterization of the pathway components functioning at different stages of the secretion process as well as analysis of the stress responses caused by impaired folding or trafficking in the pathway or by expression of heterologous proteins. Studies on the transcriptional regulation of the secretory pathway have revealed similarities, but also interesting differences, with other organisms, such as a different induction mechanism of the unfolded protein response and the repression of genes encoding secreted proteins under secretion stress conditions.
Original languageEnglish
Pages (from-to)46-57
Number of pages12
JournalMicrobiology
Volume158
Issue number1
DOIs
Publication statusPublished - 2012
MoE publication typeA2 Review article in a scientific journal

Fingerprint

Hypocrea
Trichoderma
Secretory Pathway
Proteins
Enzymes
Genome
Unfolded Protein Response
Cellulases
Laccase
Monophenol Monooxygenase
Biomass
Genes
Fermentation
Polysaccharides
Industry
Peptide Hydrolases
Fungi
Gels

Cite this

@article{1a0662a519e54350a88dfa3dcd7ce71e,
title = "The cargo and the transport system: Secreted proteins and protein secretion in Trichoderma reesei (Hypocrea jecorina)",
abstract = "Trichoderma reesei (Hypocrea jecorina) is an efficient cell factory for protein production that is exploited by the enzyme industry. Yields of over 100 g secreted protein l−1 from industrial fermentations have been reported. In this review we discuss the spectrum of proteins secreted by T. reesei and the studies carried out on its protein secretion system. The major enzymes secreted by T. reesei under production conditions are those degrading plant polysaccharides, the most dominant ones being the major cellulases, as demonstrated by the 2D gel analysis of the secretome. According to genome analysis, T. reesei has fewer genes encoding enzymes involved in plant biomass degradation compared with other fungi with sequenced genomes. We also discuss other T. reesei secreted enzymes and proteins that have been studied, such as proteases, laccase, tyrosinase and hydrophobins. Investigation of the T. reesei secretion pathway has included molecular characterization of the pathway components functioning at different stages of the secretion process as well as analysis of the stress responses caused by impaired folding or trafficking in the pathway or by expression of heterologous proteins. Studies on the transcriptional regulation of the secretory pathway have revealed similarities, but also interesting differences, with other organisms, such as a different induction mechanism of the unfolded protein response and the repression of genes encoding secreted proteins under secretion stress conditions.",
author = "Markku Saloheimo and Tiina Pakula",
year = "2012",
doi = "10.1099/mic.0.053132-0",
language = "English",
volume = "158",
pages = "46--57",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "1",

}

The cargo and the transport system : Secreted proteins and protein secretion in Trichoderma reesei (Hypocrea jecorina). / Saloheimo, Markku (Corresponding Author); Pakula, Tiina.

In: Microbiology, Vol. 158, No. 1, 2012, p. 46-57.

Research output: Contribution to journalReview ArticleScientificpeer-review

TY - JOUR

T1 - The cargo and the transport system

T2 - Secreted proteins and protein secretion in Trichoderma reesei (Hypocrea jecorina)

AU - Saloheimo, Markku

AU - Pakula, Tiina

PY - 2012

Y1 - 2012

N2 - Trichoderma reesei (Hypocrea jecorina) is an efficient cell factory for protein production that is exploited by the enzyme industry. Yields of over 100 g secreted protein l−1 from industrial fermentations have been reported. In this review we discuss the spectrum of proteins secreted by T. reesei and the studies carried out on its protein secretion system. The major enzymes secreted by T. reesei under production conditions are those degrading plant polysaccharides, the most dominant ones being the major cellulases, as demonstrated by the 2D gel analysis of the secretome. According to genome analysis, T. reesei has fewer genes encoding enzymes involved in plant biomass degradation compared with other fungi with sequenced genomes. We also discuss other T. reesei secreted enzymes and proteins that have been studied, such as proteases, laccase, tyrosinase and hydrophobins. Investigation of the T. reesei secretion pathway has included molecular characterization of the pathway components functioning at different stages of the secretion process as well as analysis of the stress responses caused by impaired folding or trafficking in the pathway or by expression of heterologous proteins. Studies on the transcriptional regulation of the secretory pathway have revealed similarities, but also interesting differences, with other organisms, such as a different induction mechanism of the unfolded protein response and the repression of genes encoding secreted proteins under secretion stress conditions.

AB - Trichoderma reesei (Hypocrea jecorina) is an efficient cell factory for protein production that is exploited by the enzyme industry. Yields of over 100 g secreted protein l−1 from industrial fermentations have been reported. In this review we discuss the spectrum of proteins secreted by T. reesei and the studies carried out on its protein secretion system. The major enzymes secreted by T. reesei under production conditions are those degrading plant polysaccharides, the most dominant ones being the major cellulases, as demonstrated by the 2D gel analysis of the secretome. According to genome analysis, T. reesei has fewer genes encoding enzymes involved in plant biomass degradation compared with other fungi with sequenced genomes. We also discuss other T. reesei secreted enzymes and proteins that have been studied, such as proteases, laccase, tyrosinase and hydrophobins. Investigation of the T. reesei secretion pathway has included molecular characterization of the pathway components functioning at different stages of the secretion process as well as analysis of the stress responses caused by impaired folding or trafficking in the pathway or by expression of heterologous proteins. Studies on the transcriptional regulation of the secretory pathway have revealed similarities, but also interesting differences, with other organisms, such as a different induction mechanism of the unfolded protein response and the repression of genes encoding secreted proteins under secretion stress conditions.

U2 - 10.1099/mic.0.053132-0

DO - 10.1099/mic.0.053132-0

M3 - Review Article

VL - 158

SP - 46

EP - 57

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 1

ER -