Vitex agnus-castus L. leaf oil, which was obtained by hydrodistillation in very low yield (0.023%), was analysed by gas chromatography and combined gas chromatography/mass spectrometry. Thirty-one compounds were identified in the oil (94.8%), of which the major components were 1, 8-cineole (50.9%), sabinene (10.8%), α-pinene (9.0%), terpinen-4-ol (4.8%), p-cymene (4.2%), limonene (2.5%) and α-terpineol (2.3%). Eight sesquiterpene hydrocarbons (2.8%) and four sesquiterpene alcohols (2.4%) were also characterized in the same oil. The oil was found to demonstrate moderate antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus.
Ekundayo, O., Laakso, I., Holopainen, M., Hiltunen, R., Oguntimein, B., & Kauppinen, V. (1990). The Chemical Composition and Antimicrobial Activity of the Leaf Oil of Vitex agnus-castus L. Journal of Essential Oil Research, 2(3), 115-119. https://doi.org/10.1080/10412905.1990.9697840