The crystal structure of an extracellular catechol oxidase from the ascomycete fungus Aspergillus oryzae

N. Hakulinen (Corresponding Author), C. Gasparetti, H. Kaljunen, Kristiina Kruus, J. Rouvinen

    Research output: Contribution to journalArticleScientificpeer-review

    30 Citations (Scopus)

    Abstract

    Catechol oxidases (EC 1.10.3.1) catalyse the oxidation of o-diphenols to their corresponding o-quinones. These oxidases contain two copper ions (CuA and CuB) within the so-called coupled type 3 copper site as found in tyrosinases (EC 1.14.18.1) and haemocyanins. The crystal structures of a limited number of bacterial and fungal tyrosinases and plant catechol oxidases have been solved. In this study, we present the first crystal structure of a fungal catechol oxidase from Aspergillus oryzae (AoCO4) at 2.5-Å resolution. AoCO4 belongs to the newly discovered family of short-tyrosinases, which are distinct from other tyrosinases and catechol oxidases because of their lack of the conserved C-terminal domain and differences in the histidine pattern for CuA. The sequence identity of AoCO4 with other structurally known enzymes is low (less than 30 %), and the crystal structure of AoCO4 diverges from that of enzymes belonging to the conventional tyrosinase family in several ways, particularly around the central α-helical core region. A diatomic oxygen moiety was identified as a bridging molecule between the two copper ions CuA and CuB separated by a distance of 4.2–4.3 Å. The UV/vis absorption spectrum of AoCO4 exhibits a distinct maximum of absorbance at 350 nm, which has been reported to be typical of the oxy form of type 3 copper enzymes.
    Original languageEnglish
    Pages (from-to)917-929
    Number of pages12
    JournalJournal of Biological Inorganic Chemistry
    Volume18
    Issue number8
    DOIs
    Publication statusPublished - 2013
    MoE publication typeA1 Journal article-refereed

    Keywords

    • binuclear copper site
    • catechol oxidase
    • type 3 copper enzymes
    • tyrosinase
    • x-ray crystallography

    Fingerprint Dive into the research topics of 'The crystal structure of an extracellular catechol oxidase from the ascomycete fungus <i>Aspergillus oryzae</i>'. Together they form a unique fingerprint.

    Cite this