The effect of 1-ethyl-3-methylimidazolium acetate on the enzymatic degradation of cellulose

G. Ebner, P. Vejdovszky, Ronny Wahlström, Anna Suurnäkki, M. Schrems, P. Kosma, T. Rosenau, A. Potthast (Corresponding Author)

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Abstract

The effect of cellulose pre-treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc) on the enzymatic hydrolysis with a cellulase from Trichoderma reesei was studied. Enzymatic assays were performed with three different pulps - cotton linters, sulfite dissolving pulp, and eucalyptus Kraft pulp. The reaction kinetics were determined by two different methods: (i) the classical test based on the measurement of released reducing sugars from a water soluble cellulosic substrate (carboxy methyl cellulose (CMC)), and (ii) a novel approach where the enzymatic activity is determined as function of the molecular weight decrease of underivatized cellulose. Furthermore the impact of the pure ionic liquid on the stability of the enzyme was investigated. We found that enzymatic degradation of cellulose I followed a completely different degradation pattern in the molecular weight distribution and sugar solubilzation compared to the corresponding regenerated cellulose II.
Original languageEnglish
Pages (from-to)121-129
Number of pages9
JournalJournal of Molecular Catalysis B: Enzymatic
Volume99
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

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Keywords

  • cellulase
  • cellulose
  • endoglucanase
  • ionic liquid
  • water content

Cite this

Ebner, G., Vejdovszky, P., Wahlström, R., Suurnäkki, A., Schrems, M., Kosma, P., Rosenau, T., & Potthast, A. (2014). The effect of 1-ethyl-3-methylimidazolium acetate on the enzymatic degradation of cellulose. Journal of Molecular Catalysis B: Enzymatic, 99, 121-129. https://doi.org/10.1016/j.molcatb.2013.11.001