The effect of 1-ethyl-3-methylimidazolium acetate on the enzymatic degradation of cellulose

G. Ebner, P. Vejdovszky, Ronny Wahlström, Anna Suurnäkki, M. Schrems, P. Kosma, T. Rosenau, A. Potthast (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

The effect of cellulose pre-treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc) on the enzymatic hydrolysis with a cellulase from Trichoderma reesei was studied. Enzymatic assays were performed with three different pulps - cotton linters, sulfite dissolving pulp, and eucalyptus Kraft pulp. The reaction kinetics were determined by two different methods: (i) the classical test based on the measurement of released reducing sugars from a water soluble cellulosic substrate (carboxy methyl cellulose (CMC)), and (ii) a novel approach where the enzymatic activity is determined as function of the molecular weight decrease of underivatized cellulose. Furthermore the impact of the pure ionic liquid on the stability of the enzyme was investigated. We found that enzymatic degradation of cellulose I followed a completely different degradation pattern in the molecular weight distribution and sugar solubilzation compared to the corresponding regenerated cellulose II.
Original languageEnglish
Pages (from-to)121-129
Number of pages9
JournalJournal of Molecular Catalysis B: Enzymatic
Volume99
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Cellulose
Ionic Liquids
Degradation
Sugars
Ionic liquids
Molecular Weight
Sulfite pulp
Enzyme Stability
Eucalyptus
Sulfites
Trichoderma
Methylcellulose
Enzymatic hydrolysis
Cellulase
Kraft pulp
Enzyme Assays
Molecular weight distribution
Reaction kinetics
Cotton
Pulp

Keywords

  • cellulase
  • cellulose
  • endoglucanase
  • ionic liquid
  • water content

Cite this

Ebner, G., Vejdovszky, P., Wahlström, R., Suurnäkki, A., Schrems, M., Kosma, P., ... Potthast, A. (2014). The effect of 1-ethyl-3-methylimidazolium acetate on the enzymatic degradation of cellulose. Journal of Molecular Catalysis B: Enzymatic, 99, 121-129. https://doi.org/10.1016/j.molcatb.2013.11.001
Ebner, G. ; Vejdovszky, P. ; Wahlström, Ronny ; Suurnäkki, Anna ; Schrems, M. ; Kosma, P. ; Rosenau, T. ; Potthast, A. / The effect of 1-ethyl-3-methylimidazolium acetate on the enzymatic degradation of cellulose. In: Journal of Molecular Catalysis B: Enzymatic. 2014 ; Vol. 99. pp. 121-129.
@article{c4ac96cb9cdf40209105b16c38263ca9,
title = "The effect of 1-ethyl-3-methylimidazolium acetate on the enzymatic degradation of cellulose",
abstract = "The effect of cellulose pre-treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc) on the enzymatic hydrolysis with a cellulase from Trichoderma reesei was studied. Enzymatic assays were performed with three different pulps - cotton linters, sulfite dissolving pulp, and eucalyptus Kraft pulp. The reaction kinetics were determined by two different methods: (i) the classical test based on the measurement of released reducing sugars from a water soluble cellulosic substrate (carboxy methyl cellulose (CMC)), and (ii) a novel approach where the enzymatic activity is determined as function of the molecular weight decrease of underivatized cellulose. Furthermore the impact of the pure ionic liquid on the stability of the enzyme was investigated. We found that enzymatic degradation of cellulose I followed a completely different degradation pattern in the molecular weight distribution and sugar solubilzation compared to the corresponding regenerated cellulose II.",
keywords = "cellulase, cellulose, endoglucanase, ionic liquid, water content",
author = "G. Ebner and P. Vejdovszky and Ronny Wahlstr{\"o}m and Anna Suurn{\"a}kki and M. Schrems and P. Kosma and T. Rosenau and A. Potthast",
year = "2014",
doi = "10.1016/j.molcatb.2013.11.001",
language = "English",
volume = "99",
pages = "121--129",
journal = "Journal of Molecular Catalysis B: Enzymatic",
issn = "1381-1177",
publisher = "Elsevier",

}

Ebner, G, Vejdovszky, P, Wahlström, R, Suurnäkki, A, Schrems, M, Kosma, P, Rosenau, T & Potthast, A 2014, 'The effect of 1-ethyl-3-methylimidazolium acetate on the enzymatic degradation of cellulose', Journal of Molecular Catalysis B: Enzymatic, vol. 99, pp. 121-129. https://doi.org/10.1016/j.molcatb.2013.11.001

The effect of 1-ethyl-3-methylimidazolium acetate on the enzymatic degradation of cellulose. / Ebner, G.; Vejdovszky, P.; Wahlström, Ronny; Suurnäkki, Anna; Schrems, M.; Kosma, P.; Rosenau, T.; Potthast, A. (Corresponding Author).

In: Journal of Molecular Catalysis B: Enzymatic, Vol. 99, 2014, p. 121-129.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The effect of 1-ethyl-3-methylimidazolium acetate on the enzymatic degradation of cellulose

AU - Ebner, G.

AU - Vejdovszky, P.

AU - Wahlström, Ronny

AU - Suurnäkki, Anna

AU - Schrems, M.

AU - Kosma, P.

AU - Rosenau, T.

AU - Potthast, A.

PY - 2014

Y1 - 2014

N2 - The effect of cellulose pre-treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc) on the enzymatic hydrolysis with a cellulase from Trichoderma reesei was studied. Enzymatic assays were performed with three different pulps - cotton linters, sulfite dissolving pulp, and eucalyptus Kraft pulp. The reaction kinetics were determined by two different methods: (i) the classical test based on the measurement of released reducing sugars from a water soluble cellulosic substrate (carboxy methyl cellulose (CMC)), and (ii) a novel approach where the enzymatic activity is determined as function of the molecular weight decrease of underivatized cellulose. Furthermore the impact of the pure ionic liquid on the stability of the enzyme was investigated. We found that enzymatic degradation of cellulose I followed a completely different degradation pattern in the molecular weight distribution and sugar solubilzation compared to the corresponding regenerated cellulose II.

AB - The effect of cellulose pre-treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc) on the enzymatic hydrolysis with a cellulase from Trichoderma reesei was studied. Enzymatic assays were performed with three different pulps - cotton linters, sulfite dissolving pulp, and eucalyptus Kraft pulp. The reaction kinetics were determined by two different methods: (i) the classical test based on the measurement of released reducing sugars from a water soluble cellulosic substrate (carboxy methyl cellulose (CMC)), and (ii) a novel approach where the enzymatic activity is determined as function of the molecular weight decrease of underivatized cellulose. Furthermore the impact of the pure ionic liquid on the stability of the enzyme was investigated. We found that enzymatic degradation of cellulose I followed a completely different degradation pattern in the molecular weight distribution and sugar solubilzation compared to the corresponding regenerated cellulose II.

KW - cellulase

KW - cellulose

KW - endoglucanase

KW - ionic liquid

KW - water content

U2 - 10.1016/j.molcatb.2013.11.001

DO - 10.1016/j.molcatb.2013.11.001

M3 - Article

VL - 99

SP - 121

EP - 129

JO - Journal of Molecular Catalysis B: Enzymatic

JF - Journal of Molecular Catalysis B: Enzymatic

SN - 1381-1177

ER -