The effect of culture conditions on the production of lignin modifying enzymes by the white-rot fungus Phlebia radiata

Marja-Leena Niku-Paavola (Corresponding Author), Eija Karhunen, Anne Kantelinen, Liisa Viikari, Taina Lundell, Annele Hatakka

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Abstract

The extracellular enzymes synthesized by Phlebia radiata Fr. 79 (ATCC 64658) under various cultivation conditions were studied in order to find out suitable enzyme combinations for the modification of lignin. The fungus produced lignin peroxidase (LiP, ligninase), manganese-dependent peroxidase (MnP) and laccase (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) in various proportions depending on the cultivation conditions. Addition of veratryl alcohol increased lignin peroxidase and manganese-dependent peroxidase activities both in agitated and non-agitated flask cultures. Laccase production was more enhanced by the addition of benzyl alcohol and veratric acid. However, the highest lignin peroxidase activities were obtained using a non-phenolic dimeric β-O-4 model compound. Pressure ground wood (PGW), chemithermomechanical pulp (CTMP) or spruce shavings in the bioreactors decreased the amount of lignin peroxidase, whereas laccase and manganese-dependent peroxidase activities increased. Lignin peroxidase and partly manganese-dependent peroxidase was adsorbed on the lignocellulose substrate, but laccase remained in the culture liquid. Here the lignin peroxidase activity was due to isozyme LiP3. In flask cultures more lignin peroxidase isozymes were secreted, the major component of which was the isozyme LiP2.
Original languageEnglish
Pages (from-to)211-221
JournalJournal of Biotechnology
Volume13
Issue number2-3
DOIs
Publication statusPublished - 1990
MoE publication typeA1 Journal article-refereed

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manganese peroxidase
Lignin
Fungi
Enzymes
Laccase
Manganese
Isoenzymes
Alcohols
Benzyl Alcohol
Bioreactors
lignin peroxidase
Pulp
Wood
Oxidoreductases
Oxygen
Pressure
Liquids
Substrates
Acids

Cite this

Niku-Paavola, Marja-Leena ; Karhunen, Eija ; Kantelinen, Anne ; Viikari, Liisa ; Lundell, Taina ; Hatakka, Annele. / The effect of culture conditions on the production of lignin modifying enzymes by the white-rot fungus Phlebia radiata. In: Journal of Biotechnology. 1990 ; Vol. 13, No. 2-3. pp. 211-221.
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abstract = "The extracellular enzymes synthesized by Phlebia radiata Fr. 79 (ATCC 64658) under various cultivation conditions were studied in order to find out suitable enzyme combinations for the modification of lignin. The fungus produced lignin peroxidase (LiP, ligninase), manganese-dependent peroxidase (MnP) and laccase (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) in various proportions depending on the cultivation conditions. Addition of veratryl alcohol increased lignin peroxidase and manganese-dependent peroxidase activities both in agitated and non-agitated flask cultures. Laccase production was more enhanced by the addition of benzyl alcohol and veratric acid. However, the highest lignin peroxidase activities were obtained using a non-phenolic dimeric β-O-4 model compound. Pressure ground wood (PGW), chemithermomechanical pulp (CTMP) or spruce shavings in the bioreactors decreased the amount of lignin peroxidase, whereas laccase and manganese-dependent peroxidase activities increased. Lignin peroxidase and partly manganese-dependent peroxidase was adsorbed on the lignocellulose substrate, but laccase remained in the culture liquid. Here the lignin peroxidase activity was due to isozyme LiP3. In flask cultures more lignin peroxidase isozymes were secreted, the major component of which was the isozyme LiP2.",
author = "Marja-Leena Niku-Paavola and Eija Karhunen and Anne Kantelinen and Liisa Viikari and Taina Lundell and Annele Hatakka",
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Niku-Paavola, M-L, Karhunen, E, Kantelinen, A, Viikari, L, Lundell, T & Hatakka, A 1990, 'The effect of culture conditions on the production of lignin modifying enzymes by the white-rot fungus Phlebia radiata', Journal of Biotechnology, vol. 13, no. 2-3, pp. 211-221. https://doi.org/10.1016/0168-1656(90)90106-L

The effect of culture conditions on the production of lignin modifying enzymes by the white-rot fungus Phlebia radiata. / Niku-Paavola, Marja-Leena (Corresponding Author); Karhunen, Eija; Kantelinen, Anne; Viikari, Liisa; Lundell, Taina; Hatakka, Annele.

In: Journal of Biotechnology, Vol. 13, No. 2-3, 1990, p. 211-221.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The effect of culture conditions on the production of lignin modifying enzymes by the white-rot fungus Phlebia radiata

AU - Niku-Paavola, Marja-Leena

AU - Karhunen, Eija

AU - Kantelinen, Anne

AU - Viikari, Liisa

AU - Lundell, Taina

AU - Hatakka, Annele

PY - 1990

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N2 - The extracellular enzymes synthesized by Phlebia radiata Fr. 79 (ATCC 64658) under various cultivation conditions were studied in order to find out suitable enzyme combinations for the modification of lignin. The fungus produced lignin peroxidase (LiP, ligninase), manganese-dependent peroxidase (MnP) and laccase (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) in various proportions depending on the cultivation conditions. Addition of veratryl alcohol increased lignin peroxidase and manganese-dependent peroxidase activities both in agitated and non-agitated flask cultures. Laccase production was more enhanced by the addition of benzyl alcohol and veratric acid. However, the highest lignin peroxidase activities were obtained using a non-phenolic dimeric β-O-4 model compound. Pressure ground wood (PGW), chemithermomechanical pulp (CTMP) or spruce shavings in the bioreactors decreased the amount of lignin peroxidase, whereas laccase and manganese-dependent peroxidase activities increased. Lignin peroxidase and partly manganese-dependent peroxidase was adsorbed on the lignocellulose substrate, but laccase remained in the culture liquid. Here the lignin peroxidase activity was due to isozyme LiP3. In flask cultures more lignin peroxidase isozymes were secreted, the major component of which was the isozyme LiP2.

AB - The extracellular enzymes synthesized by Phlebia radiata Fr. 79 (ATCC 64658) under various cultivation conditions were studied in order to find out suitable enzyme combinations for the modification of lignin. The fungus produced lignin peroxidase (LiP, ligninase), manganese-dependent peroxidase (MnP) and laccase (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) in various proportions depending on the cultivation conditions. Addition of veratryl alcohol increased lignin peroxidase and manganese-dependent peroxidase activities both in agitated and non-agitated flask cultures. Laccase production was more enhanced by the addition of benzyl alcohol and veratric acid. However, the highest lignin peroxidase activities were obtained using a non-phenolic dimeric β-O-4 model compound. Pressure ground wood (PGW), chemithermomechanical pulp (CTMP) or spruce shavings in the bioreactors decreased the amount of lignin peroxidase, whereas laccase and manganese-dependent peroxidase activities increased. Lignin peroxidase and partly manganese-dependent peroxidase was adsorbed on the lignocellulose substrate, but laccase remained in the culture liquid. Here the lignin peroxidase activity was due to isozyme LiP3. In flask cultures more lignin peroxidase isozymes were secreted, the major component of which was the isozyme LiP2.

U2 - 10.1016/0168-1656(90)90106-L

DO - 10.1016/0168-1656(90)90106-L

M3 - Article

VL - 13

SP - 211

EP - 221

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 2-3

ER -