The effect of fatty or lean fish intake on inflammatory gene expression in peripheral blood mononuclear cells of patients with coronary heart disease

Vanessa D. F. de Mello (Corresponding Author), Arja T. Erkkilä, Ursula S. Schwab, Leena Pulkkinen, Marjukka Kolehmainen, Mustafa Atalay, Hanna Mussalo, Maria Lankinen, Matej Oresic, Seppo Lehto, Matti Uusitupa

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)

Abstract

Background

Little is known about the effect of fish consumption on gene expression of inflammation-related genes in immune cells in coronary heart disease (CHD).

Aim of the study

We sought to evaluate the effect of a fatty fish (FF) or a lean fish (LF) diet on the modulation of inflammatory and endothelial function-related genes in peripheral blood mononuclear cells (PBMCs) of subjects with CHD, and its association with serum fatty acid (FA) profile and lipid metabolic compounds.

Methods

Data from 27 patients randomized into an 8-week FF (n = 10; mean ± SD: 4.3 ± 0.4 portions of fish per week), LF (n = 11; 4.7 ± 1.1 portions of fish per week), or control diet (n = 6; 0.6 ± 0.4 portions of fish per week) were analyzed. The mRNA expression was measured using real-time PCR.

Results

The effect of the intervention on the mRNA expression of the genes studied did not differ among groups. In the FF group, however, the decrease in arachidonic acid to eicosapentaenoic acid (AA:EPA) ratio in cholesterol ester and phospholipid fractions strongly correlated with the change in IL1B mRNA levels (r s = 0.60, P = 0.06 and r s = 0.86, P = 0.002, respectively). In the LF group, the decrease in palmitic acid and total saturated FAs in cholesterol esters correlated with the change in intercellular cell adhesion molecule-1 (ICAM1) expression (r s = 0.64, P = 0.04 for both). Circulating levels of soluble ICAM-1 decreased only in the LF group (P < 0.05).

Conclusions

The intake of FF or LF diet did not alter the expression of inflammatory and endothelial function-related genes in PBMCs of patients with CHD. However, the decrease in AA:EPA ratio in serum lipids in the FF group may induce an anti-inflammatory response at mRNA levels in PBMCs. A LF diet might benefit endothelial function, possibly mediated by the changes in serum FA composition.

Original languageEnglish
Pages (from-to)447-455
JournalEuropean Journal of Nutrition
Volume48
Issue number8
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Coronary Disease
Blood Cells
Fishes
Gene Expression
Diet
Messenger RNA
Eicosapentaenoic Acid
Cholesterol Esters
Intercellular Adhesion Molecule-1
Arachidonic Acid
Fatty Acids
Serum
Genes
Lipids
Metabolome
Palmitic Acid
Cell Adhesion Molecules
Real-Time Polymerase Chain Reaction
Phospholipids
Anti-Inflammatory Agents

Keywords

  • Fish
  • fatty acids
  • gene expression
  • inflammation
  • endothelial function
  • ICAM
  • IL

Cite this

de Mello, Vanessa D. F. ; Erkkilä, Arja T. ; Schwab, Ursula S. ; Pulkkinen, Leena ; Kolehmainen, Marjukka ; Atalay, Mustafa ; Mussalo, Hanna ; Lankinen, Maria ; Oresic, Matej ; Lehto, Seppo ; Uusitupa, Matti. / The effect of fatty or lean fish intake on inflammatory gene expression in peripheral blood mononuclear cells of patients with coronary heart disease. In: European Journal of Nutrition. 2009 ; Vol. 48, No. 8. pp. 447-455.
@article{874a17a7b9824cfc8912fb34aa3913ed,
title = "The effect of fatty or lean fish intake on inflammatory gene expression in peripheral blood mononuclear cells of patients with coronary heart disease",
abstract = "BackgroundLittle is known about the effect of fish consumption on gene expression of inflammation-related genes in immune cells in coronary heart disease (CHD).Aim of the studyWe sought to evaluate the effect of a fatty fish (FF) or a lean fish (LF) diet on the modulation of inflammatory and endothelial function-related genes in peripheral blood mononuclear cells (PBMCs) of subjects with CHD, and its association with serum fatty acid (FA) profile and lipid metabolic compounds.MethodsData from 27 patients randomized into an 8-week FF (n = 10; mean ± SD: 4.3 ± 0.4 portions of fish per week), LF (n = 11; 4.7 ± 1.1 portions of fish per week), or control diet (n = 6; 0.6 ± 0.4 portions of fish per week) were analyzed. The mRNA expression was measured using real-time PCR.ResultsThe effect of the intervention on the mRNA expression of the genes studied did not differ among groups. In the FF group, however, the decrease in arachidonic acid to eicosapentaenoic acid (AA:EPA) ratio in cholesterol ester and phospholipid fractions strongly correlated with the change in IL1B mRNA levels (r s = 0.60, P = 0.06 and r s = 0.86, P = 0.002, respectively). In the LF group, the decrease in palmitic acid and total saturated FAs in cholesterol esters correlated with the change in intercellular cell adhesion molecule-1 (ICAM1) expression (r s = 0.64, P = 0.04 for both). Circulating levels of soluble ICAM-1 decreased only in the LF group (P < 0.05).ConclusionsThe intake of FF or LF diet did not alter the expression of inflammatory and endothelial function-related genes in PBMCs of patients with CHD. However, the decrease in AA:EPA ratio in serum lipids in the FF group may induce an anti-inflammatory response at mRNA levels in PBMCs. A LF diet might benefit endothelial function, possibly mediated by the changes in serum FA composition.",
keywords = "Fish, fatty acids, gene expression, inflammation, endothelial function, ICAM, IL, 1{\ss}",
author = "{de Mello}, {Vanessa D. F.} and Erkkil{\"a}, {Arja T.} and Schwab, {Ursula S.} and Leena Pulkkinen and Marjukka Kolehmainen and Mustafa Atalay and Hanna Mussalo and Maria Lankinen and Matej Oresic and Seppo Lehto and Matti Uusitupa",
year = "2009",
doi = "10.1007/s00394-009-0033-y",
language = "English",
volume = "48",
pages = "447--455",
journal = "European Journal of Nutrition",
issn = "1436-6207",
publisher = "Elsevier",
number = "8",

}

de Mello, VDF, Erkkilä, AT, Schwab, US, Pulkkinen, L, Kolehmainen, M, Atalay, M, Mussalo, H, Lankinen, M, Oresic, M, Lehto, S & Uusitupa, M 2009, 'The effect of fatty or lean fish intake on inflammatory gene expression in peripheral blood mononuclear cells of patients with coronary heart disease', European Journal of Nutrition, vol. 48, no. 8, pp. 447-455. https://doi.org/10.1007/s00394-009-0033-y

The effect of fatty or lean fish intake on inflammatory gene expression in peripheral blood mononuclear cells of patients with coronary heart disease. / de Mello, Vanessa D. F. (Corresponding Author); Erkkilä, Arja T.; Schwab, Ursula S.; Pulkkinen, Leena; Kolehmainen, Marjukka; Atalay, Mustafa; Mussalo, Hanna; Lankinen, Maria; Oresic, Matej; Lehto, Seppo; Uusitupa, Matti.

In: European Journal of Nutrition, Vol. 48, No. 8, 2009, p. 447-455.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The effect of fatty or lean fish intake on inflammatory gene expression in peripheral blood mononuclear cells of patients with coronary heart disease

AU - de Mello, Vanessa D. F.

AU - Erkkilä, Arja T.

AU - Schwab, Ursula S.

AU - Pulkkinen, Leena

AU - Kolehmainen, Marjukka

AU - Atalay, Mustafa

AU - Mussalo, Hanna

AU - Lankinen, Maria

AU - Oresic, Matej

AU - Lehto, Seppo

AU - Uusitupa, Matti

PY - 2009

Y1 - 2009

N2 - BackgroundLittle is known about the effect of fish consumption on gene expression of inflammation-related genes in immune cells in coronary heart disease (CHD).Aim of the studyWe sought to evaluate the effect of a fatty fish (FF) or a lean fish (LF) diet on the modulation of inflammatory and endothelial function-related genes in peripheral blood mononuclear cells (PBMCs) of subjects with CHD, and its association with serum fatty acid (FA) profile and lipid metabolic compounds.MethodsData from 27 patients randomized into an 8-week FF (n = 10; mean ± SD: 4.3 ± 0.4 portions of fish per week), LF (n = 11; 4.7 ± 1.1 portions of fish per week), or control diet (n = 6; 0.6 ± 0.4 portions of fish per week) were analyzed. The mRNA expression was measured using real-time PCR.ResultsThe effect of the intervention on the mRNA expression of the genes studied did not differ among groups. In the FF group, however, the decrease in arachidonic acid to eicosapentaenoic acid (AA:EPA) ratio in cholesterol ester and phospholipid fractions strongly correlated with the change in IL1B mRNA levels (r s = 0.60, P = 0.06 and r s = 0.86, P = 0.002, respectively). In the LF group, the decrease in palmitic acid and total saturated FAs in cholesterol esters correlated with the change in intercellular cell adhesion molecule-1 (ICAM1) expression (r s = 0.64, P = 0.04 for both). Circulating levels of soluble ICAM-1 decreased only in the LF group (P < 0.05).ConclusionsThe intake of FF or LF diet did not alter the expression of inflammatory and endothelial function-related genes in PBMCs of patients with CHD. However, the decrease in AA:EPA ratio in serum lipids in the FF group may induce an anti-inflammatory response at mRNA levels in PBMCs. A LF diet might benefit endothelial function, possibly mediated by the changes in serum FA composition.

AB - BackgroundLittle is known about the effect of fish consumption on gene expression of inflammation-related genes in immune cells in coronary heart disease (CHD).Aim of the studyWe sought to evaluate the effect of a fatty fish (FF) or a lean fish (LF) diet on the modulation of inflammatory and endothelial function-related genes in peripheral blood mononuclear cells (PBMCs) of subjects with CHD, and its association with serum fatty acid (FA) profile and lipid metabolic compounds.MethodsData from 27 patients randomized into an 8-week FF (n = 10; mean ± SD: 4.3 ± 0.4 portions of fish per week), LF (n = 11; 4.7 ± 1.1 portions of fish per week), or control diet (n = 6; 0.6 ± 0.4 portions of fish per week) were analyzed. The mRNA expression was measured using real-time PCR.ResultsThe effect of the intervention on the mRNA expression of the genes studied did not differ among groups. In the FF group, however, the decrease in arachidonic acid to eicosapentaenoic acid (AA:EPA) ratio in cholesterol ester and phospholipid fractions strongly correlated with the change in IL1B mRNA levels (r s = 0.60, P = 0.06 and r s = 0.86, P = 0.002, respectively). In the LF group, the decrease in palmitic acid and total saturated FAs in cholesterol esters correlated with the change in intercellular cell adhesion molecule-1 (ICAM1) expression (r s = 0.64, P = 0.04 for both). Circulating levels of soluble ICAM-1 decreased only in the LF group (P < 0.05).ConclusionsThe intake of FF or LF diet did not alter the expression of inflammatory and endothelial function-related genes in PBMCs of patients with CHD. However, the decrease in AA:EPA ratio in serum lipids in the FF group may induce an anti-inflammatory response at mRNA levels in PBMCs. A LF diet might benefit endothelial function, possibly mediated by the changes in serum FA composition.

KW - Fish

KW - fatty acids

KW - gene expression

KW - inflammation

KW - endothelial function

KW - ICAM

KW - IL

KW - 1ß

U2 - 10.1007/s00394-009-0033-y

DO - 10.1007/s00394-009-0033-y

M3 - Article

VL - 48

SP - 447

EP - 455

JO - European Journal of Nutrition

JF - European Journal of Nutrition

SN - 1436-6207

IS - 8

ER -