The effect of high pH on structural lipids in diatoms

K. Spilling (Corresponding Author), A. Brynjólfsdóttir, Dagmar Enss, Heiko Rischer, H.G. Svavarsson

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)

Abstract

We tested the hypothesis that increased pH reduces the amount of structural lipids. To do this, we used three different diatoms (Phaeodactylum tricornutum CCAP strain, P. tricornutum TV strain and Amphiprora sp). We tested the effect of rapid increase from pH 7.5 to 10 by adding NaOH. The total lipid content was reduced by 13, 36 and 47 % in the P. tricornutum CCAP strain, TV strain and Amphiprora sp., respectively, 1 h after increasing the pH. The P. tricornutum CCAP strain was used for further testing the effect of pH on the lipid content during active growth. This strain was cultivated at pH 7.5 and 10, and the pH was regulated by the CO2 inflow. The growth rate was similar (0.3 day−1) in both pH treatments, but the lipid content in the pH 10 treatment was on average 28 % lower than in the pH 7.5 treatment. Our data support the hypothesis that structural lipids are reduced when pH increases to high levels. The results suggest that regulating the pH during algae cultivation could be used to refine the lipid composition in the harvested algal biomass.
Original languageEnglish
Pages (from-to)1435-1439
Number of pages5
JournalJournal of Applied Phycology
Volume25
Issue number5
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Bacillariophyceae
diatom
lipid
Phaeodactylum tricornutum
lipids
Amphiprora
lipid content
algae
lipid composition
effect
inflow
alga
biomass

Keywords

  • glycolipids
  • membrane lipids
  • phospholipids
  • polyunsaturated fatty acids

Cite this

Spilling, K., Brynjólfsdóttir, A., Enss, D., Rischer, H., & Svavarsson, H. G. (2013). The effect of high pH on structural lipids in diatoms. Journal of Applied Phycology, 25(5), 1435-1439. https://doi.org/10.1007/s10811-012-9971-5
Spilling, K. ; Brynjólfsdóttir, A. ; Enss, Dagmar ; Rischer, Heiko ; Svavarsson, H.G. / The effect of high pH on structural lipids in diatoms. In: Journal of Applied Phycology. 2013 ; Vol. 25, No. 5. pp. 1435-1439.
@article{cbe14a8ea3b04f5a98124e232e4a7917,
title = "The effect of high pH on structural lipids in diatoms",
abstract = "We tested the hypothesis that increased pH reduces the amount of structural lipids. To do this, we used three different diatoms (Phaeodactylum tricornutum CCAP strain, P. tricornutum TV strain and Amphiprora sp). We tested the effect of rapid increase from pH 7.5 to 10 by adding NaOH. The total lipid content was reduced by 13, 36 and 47 {\%} in the P. tricornutum CCAP strain, TV strain and Amphiprora sp., respectively, 1 h after increasing the pH. The P. tricornutum CCAP strain was used for further testing the effect of pH on the lipid content during active growth. This strain was cultivated at pH 7.5 and 10, and the pH was regulated by the CO2 inflow. The growth rate was similar (0.3 day−1) in both pH treatments, but the lipid content in the pH 10 treatment was on average 28 {\%} lower than in the pH 7.5 treatment. Our data support the hypothesis that structural lipids are reduced when pH increases to high levels. The results suggest that regulating the pH during algae cultivation could be used to refine the lipid composition in the harvested algal biomass.",
keywords = "glycolipids, membrane lipids, phospholipids, polyunsaturated fatty acids",
author = "K. Spilling and A. Brynj{\'o}lfsd{\'o}ttir and Dagmar Enss and Heiko Rischer and H.G. Svavarsson",
year = "2013",
doi = "10.1007/s10811-012-9971-5",
language = "English",
volume = "25",
pages = "1435--1439",
journal = "Journal of Applied Phycology",
issn = "0921-8971",
publisher = "Springer",
number = "5",

}

Spilling, K, Brynjólfsdóttir, A, Enss, D, Rischer, H & Svavarsson, HG 2013, 'The effect of high pH on structural lipids in diatoms', Journal of Applied Phycology, vol. 25, no. 5, pp. 1435-1439. https://doi.org/10.1007/s10811-012-9971-5

The effect of high pH on structural lipids in diatoms. / Spilling, K. (Corresponding Author); Brynjólfsdóttir, A.; Enss, Dagmar; Rischer, Heiko; Svavarsson, H.G.

In: Journal of Applied Phycology, Vol. 25, No. 5, 2013, p. 1435-1439.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The effect of high pH on structural lipids in diatoms

AU - Spilling, K.

AU - Brynjólfsdóttir, A.

AU - Enss, Dagmar

AU - Rischer, Heiko

AU - Svavarsson, H.G.

PY - 2013

Y1 - 2013

N2 - We tested the hypothesis that increased pH reduces the amount of structural lipids. To do this, we used three different diatoms (Phaeodactylum tricornutum CCAP strain, P. tricornutum TV strain and Amphiprora sp). We tested the effect of rapid increase from pH 7.5 to 10 by adding NaOH. The total lipid content was reduced by 13, 36 and 47 % in the P. tricornutum CCAP strain, TV strain and Amphiprora sp., respectively, 1 h after increasing the pH. The P. tricornutum CCAP strain was used for further testing the effect of pH on the lipid content during active growth. This strain was cultivated at pH 7.5 and 10, and the pH was regulated by the CO2 inflow. The growth rate was similar (0.3 day−1) in both pH treatments, but the lipid content in the pH 10 treatment was on average 28 % lower than in the pH 7.5 treatment. Our data support the hypothesis that structural lipids are reduced when pH increases to high levels. The results suggest that regulating the pH during algae cultivation could be used to refine the lipid composition in the harvested algal biomass.

AB - We tested the hypothesis that increased pH reduces the amount of structural lipids. To do this, we used three different diatoms (Phaeodactylum tricornutum CCAP strain, P. tricornutum TV strain and Amphiprora sp). We tested the effect of rapid increase from pH 7.5 to 10 by adding NaOH. The total lipid content was reduced by 13, 36 and 47 % in the P. tricornutum CCAP strain, TV strain and Amphiprora sp., respectively, 1 h after increasing the pH. The P. tricornutum CCAP strain was used for further testing the effect of pH on the lipid content during active growth. This strain was cultivated at pH 7.5 and 10, and the pH was regulated by the CO2 inflow. The growth rate was similar (0.3 day−1) in both pH treatments, but the lipid content in the pH 10 treatment was on average 28 % lower than in the pH 7.5 treatment. Our data support the hypothesis that structural lipids are reduced when pH increases to high levels. The results suggest that regulating the pH during algae cultivation could be used to refine the lipid composition in the harvested algal biomass.

KW - glycolipids

KW - membrane lipids

KW - phospholipids

KW - polyunsaturated fatty acids

U2 - 10.1007/s10811-012-9971-5

DO - 10.1007/s10811-012-9971-5

M3 - Article

VL - 25

SP - 1435

EP - 1439

JO - Journal of Applied Phycology

JF - Journal of Applied Phycology

SN - 0921-8971

IS - 5

ER -