The effect of lactulose on the survival of Lactobacillus rhamnosus in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and in vivo

Pia Kontula, Lode Nollet, Maria Saarela, Terttu Vilpponen-Salmela, Willy Verstraete, Tiina Mattila-Sandholm, Atte von Wright (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

15 Citations (Scopus)

Abstract

The effect of lactulose on the survival of Lactobacillus rhamnosus VTT E-97800 and on the colon microbiota and its metabolic activity was studied using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) model. L. rhamnosus VTT E-97800 and lactulose together enhanced the production of butyric acid and decreased ammonium concentration in the model.
The numbers of Bacteroides were observed to decrease 2.5-2.0 log 10 cfu/ml during the SHIME experiment. In the subsequent in vivo studies the colonisation of the human colon by L. rhamnosus VTT E-97800 could be confirmed using either RAPD or RiboPrinter™ for the strain identification. RAPD showed that 10-60% more L. rhamnosus VTT E-97800 was detected in lactulose subjects compared with controls after strain feeding was discontinued indicating that lactulose might enhance the survival of the strain in the colon.
Original languageEnglish
Pages (from-to)90-96
JournalMicrobial Ecology in Health and Disease
Volume14
Issue number2
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Lactobacillus rhamnosus
Lactulose
Ecosystem
Colon
Survival
Bacteroides
Butyric Acid
Microbiota
Ammonium Compounds

Keywords

  • gastrointestinal (GI) microbiota
  • microflora
  • gut microflora
  • ecology
  • intestinal microbiota
  • human health
  • Lactobacillus
  • Lactobacillus rhamnosus
  • lactulose

Cite this

Kontula, Pia ; Nollet, Lode ; Saarela, Maria ; Vilpponen-Salmela, Terttu ; Verstraete, Willy ; Mattila-Sandholm, Tiina ; von Wright, Atte. / The effect of lactulose on the survival of Lactobacillus rhamnosus in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and in vivo. In: Microbial Ecology in Health and Disease. 2002 ; Vol. 14, No. 2. pp. 90-96.
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abstract = "The effect of lactulose on the survival of Lactobacillus rhamnosus VTT E-97800 and on the colon microbiota and its metabolic activity was studied using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) model. L. rhamnosus VTT E-97800 and lactulose together enhanced the production of butyric acid and decreased ammonium concentration in the model. The numbers of Bacteroides were observed to decrease 2.5-2.0 log 10 cfu/ml during the SHIME experiment. In the subsequent in vivo studies the colonisation of the human colon by L. rhamnosus VTT E-97800 could be confirmed using either RAPD or RiboPrinter™ for the strain identification. RAPD showed that 10-60{\%} more L. rhamnosus VTT E-97800 was detected in lactulose subjects compared with controls after strain feeding was discontinued indicating that lactulose might enhance the survival of the strain in the colon.",
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The effect of lactulose on the survival of Lactobacillus rhamnosus in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and in vivo. / Kontula, Pia; Nollet, Lode; Saarela, Maria; Vilpponen-Salmela, Terttu; Verstraete, Willy; Mattila-Sandholm, Tiina; von Wright, Atte (Corresponding Author).

In: Microbial Ecology in Health and Disease, Vol. 14, No. 2, 2002, p. 90-96.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Nollet, Lode

AU - Saarela, Maria

AU - Vilpponen-Salmela, Terttu

AU - Verstraete, Willy

AU - Mattila-Sandholm, Tiina

AU - von Wright, Atte

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