The effect of laser irradiation on living cells incubated with gold nanoparticles

Olga Bibikova, Prateek Singh, Alexey Popov, Georgy Akchurin, Ilya Skovorodkin, Vitaly Khanadeev, Matti Kinnunen, Vladimir Bogatyrev, Nikolai Khlebtsov, Seppo Vainio, Valery Tuchin

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

2 Citations (Scopus)

Abstract

Plasmon-resonant nanoparticles attached to cell membranes, under laser treatment can temporarily increase membrane permeability. In this paper, the influence of continuous-wave and pulsed (nanosecond) laser irradiation on living cells incubated with gold nanoparticles was investigated. Gold nanospheres, nanostars, and nanorods with different functionalization were used as plasmonic agents. The dependence between increase of medium temperature on the irradiation time was showed for nanostars and nanorods with different surface properties. Cells samples incubated with gold nanorods showed the highest temperature increase. Feasibility of cell optoporation by the use of gold nanospheres with variable functionalization was demonstrated. The cell membrane permeability was successfully enhanced as shown by the uptake of the fluorescent dye upon nanosecond laser treatment. Toxicity of the nanoparticles was estimated by MTT assay.

Original languageEnglish
Title of host publicationPlasmonics in Biology and Medicine XII
EditorsJoseph R. Lakowicz, Tuan Vo-Dinh
PublisherInternational Society for Optics and Photonics SPIE
ISBN (Print)978-1-6284-1430-1
DOIs
Publication statusPublished - 1 Jan 2015
MoE publication typeNot Eligible
EventPlasmonics in Biology and Medicine XII - San Francisco, United States
Duration: 7 Feb 20158 Feb 2015

Publication series

SeriesProgress in Biomedical Optics and Imaging
Number38
Volume16
ISSN1605-7422
SeriesProceedings of SPIE
Volume9340
ISSN0277-786X

Conference

ConferencePlasmonics in Biology and Medicine XII
CountryUnited States
CitySan Francisco
Period7/02/158/02/15

Keywords

  • Continuous wave laser
  • Irradiation
  • Nanorods
  • Nanosecond laser
  • Nanospheres
  • Nanostars
  • Optoporation
  • Plasmon-resonant gold nanoparticles

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  • Cite this

    Bibikova, O., Singh, P., Popov, A., Akchurin, G., Skovorodkin, I., Khanadeev, V., Kinnunen, M., Bogatyrev, V., Khlebtsov, N., Vainio, S., & Tuchin, V. (2015). The effect of laser irradiation on living cells incubated with gold nanoparticles. In J. R. Lakowicz, & T. Vo-Dinh (Eds.), Plasmonics in Biology and Medicine XII [934010-1] International Society for Optics and Photonics SPIE. Progress in Biomedical Optics and Imaging, No. 38, Vol.. 16, Proceedings of SPIE, Vol.. 9340 https://doi.org/10.1117/12.2083023