The effect of oxidative pretreatment on cellulose degradation by Poria placenta and Trichoderma reesei cellulases

Marjaana Rättö, Anne-Christine Ritschkoff

Research output: Contribution to journalArticleScientificpeer-review

41 Citations (Scopus)

Abstract

The possible role of hydrogen peroxide in brown-rot decay was investigated by studying the effects of pretreatment of spruce wood and microcrystalline Avicel cellulose with H2O2 and Fe2+ (Fenton's reagent) on the subsequent enzymatic hydrolysis of the substrates. A crude endoglucanase preparation from the brown-rot fungus Poria placenta, a purified endoglucanase from Trichoderma reesei and a commercial Trichoderma cellulase were used as enzymes. Avicel cellulose and spruce dust were depolymerized in the H2O2/Fe2+ treatment. Mainly hemicelluloses were lost in the treatment of spruce dust. The effect of the pretreatment on subsequent enzymatic hydrolysis was found to depend on the nature of the substrate and the enzyme preparation used. Pretreatment with H2O2/Fe2+ clearly increased the amount of enzymatic hydrolysis of spruce dust with both the endoglucanases and the commercial cellulase. In all cases the amount of hydrolysis was increased about threefold. The hydrolysis of Avicel with the endoglucanases was also enhanced, whereas the hydrolysis with the commercial cellulase was decreased.

Original languageEnglish
Pages (from-to)53 - 57
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume48
Issue number1
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

Fingerprint

Poria
Cellulases
Trichoderma
Cellulase
Cellulose
Placenta
Hydrolysis
Dust
Enzymes
Hydrogen Peroxide
Fungi

Cite this

@article{f51f7ea774184b40bc87beb7b92546bc,
title = "The effect of oxidative pretreatment on cellulose degradation by Poria placenta and Trichoderma reesei cellulases",
abstract = "The possible role of hydrogen peroxide in brown-rot decay was investigated by studying the effects of pretreatment of spruce wood and microcrystalline Avicel cellulose with H2O2 and Fe2+ (Fenton's reagent) on the subsequent enzymatic hydrolysis of the substrates. A crude endoglucanase preparation from the brown-rot fungus Poria placenta, a purified endoglucanase from Trichoderma reesei and a commercial Trichoderma cellulase were used as enzymes. Avicel cellulose and spruce dust were depolymerized in the H2O2/Fe2+ treatment. Mainly hemicelluloses were lost in the treatment of spruce dust. The effect of the pretreatment on subsequent enzymatic hydrolysis was found to depend on the nature of the substrate and the enzyme preparation used. Pretreatment with H2O2/Fe2+ clearly increased the amount of enzymatic hydrolysis of spruce dust with both the endoglucanases and the commercial cellulase. In all cases the amount of hydrolysis was increased about threefold. The hydrolysis of Avicel with the endoglucanases was also enhanced, whereas the hydrolysis with the commercial cellulase was decreased.",
author = "Marjaana R{\"a}tt{\"o} and Anne-Christine Ritschkoff",
year = "1997",
doi = "10.1007/s002530051014",
language = "English",
volume = "48",
pages = "53 -- 57",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer",
number = "1",

}

The effect of oxidative pretreatment on cellulose degradation by Poria placenta and Trichoderma reesei cellulases. / Rättö, Marjaana; Ritschkoff, Anne-Christine.

In: Applied Microbiology and Biotechnology, Vol. 48, No. 1, 1997, p. 53 - 57.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The effect of oxidative pretreatment on cellulose degradation by Poria placenta and Trichoderma reesei cellulases

AU - Rättö, Marjaana

AU - Ritschkoff, Anne-Christine

PY - 1997

Y1 - 1997

N2 - The possible role of hydrogen peroxide in brown-rot decay was investigated by studying the effects of pretreatment of spruce wood and microcrystalline Avicel cellulose with H2O2 and Fe2+ (Fenton's reagent) on the subsequent enzymatic hydrolysis of the substrates. A crude endoglucanase preparation from the brown-rot fungus Poria placenta, a purified endoglucanase from Trichoderma reesei and a commercial Trichoderma cellulase were used as enzymes. Avicel cellulose and spruce dust were depolymerized in the H2O2/Fe2+ treatment. Mainly hemicelluloses were lost in the treatment of spruce dust. The effect of the pretreatment on subsequent enzymatic hydrolysis was found to depend on the nature of the substrate and the enzyme preparation used. Pretreatment with H2O2/Fe2+ clearly increased the amount of enzymatic hydrolysis of spruce dust with both the endoglucanases and the commercial cellulase. In all cases the amount of hydrolysis was increased about threefold. The hydrolysis of Avicel with the endoglucanases was also enhanced, whereas the hydrolysis with the commercial cellulase was decreased.

AB - The possible role of hydrogen peroxide in brown-rot decay was investigated by studying the effects of pretreatment of spruce wood and microcrystalline Avicel cellulose with H2O2 and Fe2+ (Fenton's reagent) on the subsequent enzymatic hydrolysis of the substrates. A crude endoglucanase preparation from the brown-rot fungus Poria placenta, a purified endoglucanase from Trichoderma reesei and a commercial Trichoderma cellulase were used as enzymes. Avicel cellulose and spruce dust were depolymerized in the H2O2/Fe2+ treatment. Mainly hemicelluloses were lost in the treatment of spruce dust. The effect of the pretreatment on subsequent enzymatic hydrolysis was found to depend on the nature of the substrate and the enzyme preparation used. Pretreatment with H2O2/Fe2+ clearly increased the amount of enzymatic hydrolysis of spruce dust with both the endoglucanases and the commercial cellulase. In all cases the amount of hydrolysis was increased about threefold. The hydrolysis of Avicel with the endoglucanases was also enhanced, whereas the hydrolysis with the commercial cellulase was decreased.

U2 - 10.1007/s002530051014

DO - 10.1007/s002530051014

M3 - Article

VL - 48

SP - 53

EP - 57

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 1

ER -