The effect of probiotic strains on the microbiota of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME)

Minna Alander (Corresponding Author), I. de Smith, L. Nollet, W. Verstraete, Atte von Wright, Tiina Mattila-Sandholm

Research output: Contribution to journalArticleScientificpeer-review

80 Citations (Scopus)

Abstract

The aim of the present work was to study five potential probiotic strains (Lactobacillus plantarum, two strains of L. paracasei subsp. paracasei, L. rhamnosus and Bifidobacterium sp.) comparatively in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) in vitro model, and to evaluate this model as a tool in the screening and selection of probiotic bacteria. The impact of the strains on the composition of microbiota and its metabolic activities (production of lactic acid and short-chain fatty acids) was studied. Changes in composition of the microbiota become apparent as a result of probiotic treatment. A marked, but temporary, increase was noted in the number of lactic acid bacteria and bifidobacteria. The profiles of D(−) and L(+) isomers of lactic acid detected in the SHIME after addition of probiotic strains corresponded well to those that are produced in pure culture conditions. The numbers of enterobacteriaceae decreased markedly and those of clostridia detectably during the intervention, while the enterococci tended to increase after the treatment. This pattern was similar in the reactors representing both the small and large intestine in the model. The changes in short-chain fatty acids were small, and no definite trend was observed.
Original languageEnglish
Pages (from-to)71-79
Number of pages9
JournalInternational Journal of Food Microbiology
Volume46
Issue number1
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

Fingerprint

microbial ecology
Microbiota
Probiotics
probiotics
Ecosystem
Lactic Acid
Bifidobacterium
Volatile Fatty Acids
short chain fatty acids
lactic acid
Lactobacillus paracasei subsp. paracasei
Bacteria
Lactobacillus plantarum
Lactobacillus rhamnosus
Clostridium
Large Intestine
Enterococcus
large intestine
Enterobacteriaceae
Small Intestine

Cite this

Alander, Minna ; de Smith, I. ; Nollet, L. ; Verstraete, W. ; Wright, Atte von ; Mattila-Sandholm, Tiina. / The effect of probiotic strains on the microbiota of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME). In: International Journal of Food Microbiology. 1999 ; Vol. 46, No. 1. pp. 71-79.
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The effect of probiotic strains on the microbiota of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME). / Alander, Minna (Corresponding Author); de Smith, I.; Nollet, L.; Verstraete, W.; Wright, Atte von; Mattila-Sandholm, Tiina.

In: International Journal of Food Microbiology, Vol. 46, No. 1, 1999, p. 71-79.

Research output: Contribution to journalArticleScientificpeer-review

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