The effect of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by the red unicellular alga Porphyridium purpureum

Anna-Maria Nuutila (Corresponding Author), Anna-Marja Aura, Martti Kiesvaara, Veli Kauppinen

Research output: Contribution to journalArticleScientificpeer-review

24 Citations (Scopus)

Abstract

The effects of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by Porphyridium purpureum were studied. Both systematic and stepwise optimization were used in order to determine the best conditions for production of EPA. An analysis method for fatty acids was also developed. The highest EPA production per cell was achieved at pH 7.6 with 41.8 g l−1 NaCl and 12.1 mM nitrate in the production medium and using a growth temperature of 8°C.
Original languageEnglish
Pages (from-to)55 - 63
JournalJournal of Biotechnology
Volume55
Issue number1
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

Fingerprint

lissamine rhodamine B
Porphyridium
Rhodophyta
Eicosapentaenoic Acid
Salinity
Algae
Nitrates
Temperature
Acids
Growth temperature
Fatty Acids
Fatty acids
Growth

Keywords

  • Eicosapentaenoic acid
  • Optimization of production conditions
  • Porphyridium purpureum

Cite this

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title = "The effect of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by the red unicellular alga Porphyridium purpureum",
abstract = "The effects of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by Porphyridium purpureum were studied. Both systematic and stepwise optimization were used in order to determine the best conditions for production of EPA. An analysis method for fatty acids was also developed. The highest EPA production per cell was achieved at pH 7.6 with 41.8 g l−1 NaCl and 12.1 mM nitrate in the production medium and using a growth temperature of 8°C.",
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The effect of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by the red unicellular alga Porphyridium purpureum. / Nuutila, Anna-Maria (Corresponding Author); Aura, Anna-Marja; Kiesvaara, Martti; Kauppinen, Veli.

In: Journal of Biotechnology, Vol. 55, No. 1, 1997, p. 55 - 63.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The effect of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by the red unicellular alga Porphyridium purpureum

AU - Nuutila, Anna-Maria

AU - Aura, Anna-Marja

AU - Kiesvaara, Martti

AU - Kauppinen, Veli

PY - 1997

Y1 - 1997

N2 - The effects of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by Porphyridium purpureum were studied. Both systematic and stepwise optimization were used in order to determine the best conditions for production of EPA. An analysis method for fatty acids was also developed. The highest EPA production per cell was achieved at pH 7.6 with 41.8 g l−1 NaCl and 12.1 mM nitrate in the production medium and using a growth temperature of 8°C.

AB - The effects of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by Porphyridium purpureum were studied. Both systematic and stepwise optimization were used in order to determine the best conditions for production of EPA. An analysis method for fatty acids was also developed. The highest EPA production per cell was achieved at pH 7.6 with 41.8 g l−1 NaCl and 12.1 mM nitrate in the production medium and using a growth temperature of 8°C.

KW - Eicosapentaenoic acid

KW - Optimization of production conditions

KW - Porphyridium purpureum

U2 - 10.1016/S0168-1656(97)00060-6

DO - 10.1016/S0168-1656(97)00060-6

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