The effect of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by the red unicellular alga Porphyridium purpureum

Anna-Maria Nuutila (Corresponding Author), Anna-Marja Aura, Martti Kiesvaara, Veli Kauppinen

    Research output: Contribution to journalArticleScientificpeer-review

    27 Citations (Scopus)

    Abstract

    The effects of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by Porphyridium purpureum were studied. Both systematic and stepwise optimization were used in order to determine the best conditions for production of EPA. An analysis method for fatty acids was also developed. The highest EPA production per cell was achieved at pH 7.6 with 41.8 g l−1 NaCl and 12.1 mM nitrate in the production medium and using a growth temperature of 8°C.
    Original languageEnglish
    Pages (from-to)55 - 63
    JournalJournal of Biotechnology
    Volume55
    Issue number1
    DOIs
    Publication statusPublished - 1997
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Eicosapentaenoic acid
    • Optimization of production conditions
    • Porphyridium purpureum

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