The effects of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by Porphyridium purpureum were studied. Both systematic and stepwise optimization were used in order to determine the best conditions for production of EPA. An analysis method for fatty acids was also developed. The highest EPA production per cell was achieved at pH 7.6 with 41.8 g l−1 NaCl and 12.1 mM nitrate in the production medium and using a growth temperature of 8°C.
- Eicosapentaenoic acid
- Optimization of production conditions
- Porphyridium purpureum