The effect of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by the red unicellular alga Porphyridium purpureum

Anna-Maria Nuutila (Corresponding Author), Anna-Marja Aura, Martti Kiesvaara, Veli Kauppinen

Research output: Contribution to journalArticleScientificpeer-review

25 Citations (Scopus)

Abstract

The effects of salinity, nitrate concentration, pH and temperature on eicosapentaenoic acid (EPA) production by Porphyridium purpureum were studied. Both systematic and stepwise optimization were used in order to determine the best conditions for production of EPA. An analysis method for fatty acids was also developed. The highest EPA production per cell was achieved at pH 7.6 with 41.8 g l−1 NaCl and 12.1 mM nitrate in the production medium and using a growth temperature of 8°C.
Original languageEnglish
Pages (from-to)55 - 63
JournalJournal of Biotechnology
Volume55
Issue number1
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

Keywords

  • Eicosapentaenoic acid
  • Optimization of production conditions
  • Porphyridium purpureum

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