The effects of calcium chloride and sucrose prefreezing treatments on the structure of strawberry tissues

Jaana Suutarinen, Kaisu Heiska, Patricia Moss, Karin Autio

Research output: Contribution to journalArticleScientificpeer-review

42 Citations (Scopus)

Abstract

The structural changes in strawberry tissues during prefreezing treatments, freezing and thawing were studied by means of textural and drip loss measurements as well as by bright-field and confocal laser scanning microscopy (CLSM), and by Fourier transform infrared (FT-IR) microscopy. Calcium chloride or sucrose were used as dipping pretreatment agents before freezing. In the calcium chloride test series a full factorial screening design was used. The three-level factors included were the CaCl2concentration of the dipping solution, the dipping time and the temperature of the solution. Two kinds of sucrose treatments were used. Strawberries were either dipped in water–sucrose solutions or were sprinkled with crystallized sucrose. The CaCl2or sucrose pretreatments did not significantly affect the drip loss of thawed strawberries compared to water dips or untreated reference samples. The pretreated strawberries were firmer than the untreated control samples but some of the CaCl2- and sucrose-treated strawberries were less firm than the strawberries dipped in water. According to microscopical studies, both CaCl2and crystallized sucrose pretreatments affected the microstructure of strawberry tissues. These pretreatments especially affected pectin, protein, lignin and structural carbohydrates in the vascular tissue and cortex compared to the untreated reference samples. CaCl2-treatment had an even stronger effect than sucrose on the above compounds except lignin. The pretreatments did not seem to affect the epidermis, hypodermis or pith.
Original languageEnglish
Pages (from-to)89 - 102
Number of pages14
JournalLWT - Food Science and Technology
Volume33
Issue number2
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

Fingerprint

Fragaria
Calcium Chloride
calcium chloride
strawberries
Sucrose
sucrose
pretreatment
dipping
Lignin
drip loss
Freezing
lignin
freezing
Water
Subcutaneous Tissue
confocal laser scanning microscopy
pith
vascular tissues
Fourier Analysis
epidermis (plant)

Cite this

Suutarinen, Jaana ; Heiska, Kaisu ; Moss, Patricia ; Autio, Karin. / The effects of calcium chloride and sucrose prefreezing treatments on the structure of strawberry tissues. In: LWT - Food Science and Technology. 2000 ; Vol. 33, No. 2. pp. 89 - 102.
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abstract = "The structural changes in strawberry tissues during prefreezing treatments, freezing and thawing were studied by means of textural and drip loss measurements as well as by bright-field and confocal laser scanning microscopy (CLSM), and by Fourier transform infrared (FT-IR) microscopy. Calcium chloride or sucrose were used as dipping pretreatment agents before freezing. In the calcium chloride test series a full factorial screening design was used. The three-level factors included were the CaCl2concentration of the dipping solution, the dipping time and the temperature of the solution. Two kinds of sucrose treatments were used. Strawberries were either dipped in water–sucrose solutions or were sprinkled with crystallized sucrose. The CaCl2or sucrose pretreatments did not significantly affect the drip loss of thawed strawberries compared to water dips or untreated reference samples. The pretreated strawberries were firmer than the untreated control samples but some of the CaCl2- and sucrose-treated strawberries were less firm than the strawberries dipped in water. According to microscopical studies, both CaCl2and crystallized sucrose pretreatments affected the microstructure of strawberry tissues. These pretreatments especially affected pectin, protein, lignin and structural carbohydrates in the vascular tissue and cortex compared to the untreated reference samples. CaCl2-treatment had an even stronger effect than sucrose on the above compounds except lignin. The pretreatments did not seem to affect the epidermis, hypodermis or pith.",
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The effects of calcium chloride and sucrose prefreezing treatments on the structure of strawberry tissues. / Suutarinen, Jaana; Heiska, Kaisu; Moss, Patricia; Autio, Karin.

In: LWT - Food Science and Technology, Vol. 33, No. 2, 2000, p. 89 - 102.

Research output: Contribution to journalArticleScientificpeer-review

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AB - The structural changes in strawberry tissues during prefreezing treatments, freezing and thawing were studied by means of textural and drip loss measurements as well as by bright-field and confocal laser scanning microscopy (CLSM), and by Fourier transform infrared (FT-IR) microscopy. Calcium chloride or sucrose were used as dipping pretreatment agents before freezing. In the calcium chloride test series a full factorial screening design was used. The three-level factors included were the CaCl2concentration of the dipping solution, the dipping time and the temperature of the solution. Two kinds of sucrose treatments were used. Strawberries were either dipped in water–sucrose solutions or were sprinkled with crystallized sucrose. The CaCl2or sucrose pretreatments did not significantly affect the drip loss of thawed strawberries compared to water dips or untreated reference samples. The pretreated strawberries were firmer than the untreated control samples but some of the CaCl2- and sucrose-treated strawberries were less firm than the strawberries dipped in water. According to microscopical studies, both CaCl2and crystallized sucrose pretreatments affected the microstructure of strawberry tissues. These pretreatments especially affected pectin, protein, lignin and structural carbohydrates in the vascular tissue and cortex compared to the untreated reference samples. CaCl2-treatment had an even stronger effect than sucrose on the above compounds except lignin. The pretreatments did not seem to affect the epidermis, hypodermis or pith.

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