The effects of drugs inhibiting protein secretion in the filamentous fungus Trichoderma reesei: Evidence for down-regulation of genes that encode secreted proteins in the stressed cells

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    110 Citations (Scopus)

    Abstract

    To study the mechanisms of protein secretion as well as the cellular responses to impaired protein folding and transport in filamentous fungi, we have analyzed Trichoderma reesei cultures treated with chemical agents that interfere with these processes, dithiothreitol, brefeldin A, and the Ca 2+-ionophore A23187. The effects of the drugs on the kinetics of protein synthesis and transport were characterized using metabolic labeling of synthesized proteins. Cellobiohydrolase I (CBHI, Cel7A), the major secreted cellulase, was analyzed as a model protein. Northern analysis showed that under conditions where protein transport was inhibited (treatments with dithiothreitol or brefeldin A) the unfolded protein response pathway was activated. The active form of the hac1 mRNA that mediates unfolded protein response signaling was induced, followed by induction of the foldase and chaperone genes pdi1 and bip1. Concomitant with the activation of the unfolded protein response pathway, the transcript levels of genes encoding secreted proteins, like cellulases and xylanases, were drastically decreased, suggesting a novel type of feedback mechanism activated in response to impairment in protein folding or transport (repression under secretion stress (RESS)). By studying expression of the reporter gene lacZ under cbh1 promoters of different length, it was shown that the feedback response was mediated through the cellulase promoter.

    Original languageEnglish
    Pages (from-to)45011-45020
    Number of pages10
    JournalJournal of Biological Chemistry
    Volume278
    Issue number45
    DOIs
    Publication statusPublished - 7 Nov 2003
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Trichoderma reesei
    • filamentous fungi
    • inhibitors
    • proteinase inhibitors

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