The effects of pH oscillation on Lactobacillus rhamnosus batch cultivation

Janne Wallenius (Corresponding Author), Dorothee Barth, Tero Eerikäinen

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)


Inhomogeneous mixing in industrial-sized fermentation processes causes oscillations in process parameters such as temperature or pH value in the cultivation medium, which causes stress to the bacteria being cultivated. In this work, the impact of extracellular pH oscillations on the production of Lactobacillus rhamnosus, a well-studied probiotic bacteria, were investigated by means of a scale-down batch process, simulating inhomogeneous pH values by controlling the pH value of the medium on sinusoidal trajectories. Effects of pH stimulation on the bacteria were assessed by testing storage and freeze-drying stability of harvested cells, two factors relevant for the industrial process. Furthermore, gene expressions of six selected genes, i.e. atpA, fat, cfa, groEL, hrcA, and pstS, known to be related to stress response were monitored. Although storage stability is only slightly negatively affected by pH stimulation of the bacteria, gene expression of four of the studied genes, i.e. fat, hrcA, groEL, and pstS show to correlate with amplitude and frequency of the oscillation.
Original languageEnglish
Pages (from-to)1265-1273
Number of pages9
JournalApplied Microbiology and Biotechnology
Issue number5
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed


  • Gene expression
  • Oscillatory pH stimuli
  • Freezing
  • Freeze drying
  • Lactobacillus rhamnosus
  • qPCR
  • atpA
  • fat
  • cfa
  • groEL
  • hrcA
  • pstS
  • ldhD

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