The free and carrier-bound yeast populations from a two-stage immobilised yeast reactor are in different physiological condition

Virve Vidgren; Anne Huuskonen; Ilkka Virkajärvi; Heini Koivistoinen; John Londesborough

The free and carrier-bound yeast populations from a two-stage immobilised yeast reactor are in different physiological condition

Virve Vidgren, Anne Huuskonen, Ilkka Virkajärvi, Heini Koivistoinen, John Londesborough

Research output: Contribution to conference › Conference article › Scientific

Abstract

To better understand the operation of a 2-stage immobilised yeast reactor making beer from wort, different yeast populations from the first stage were analysed. Yeast amounts (mg fresh yeast/g wood chip) were about: free, 10; loosely bound, 78; tightly bound, 40. Proteomics showed that from the 300 most abundant soluble proteins 38 had at least 2-fold different abundancies in free and loosely bound yeast. Some of these variable proteins have been identified. Loosely bound yeast contained less sterol and less unsaturated fatty acids than free yeast. Maltose permease activity was two-fold greater in free than loosely bound yeast.

Original language	English
Publication status	Published - 2004
MoE publication type	Not Eligible
Event	NORFA Yeast Systems Biology Workshop - Copenhagen, Denmark Duration: 17 Nov 2004 → 21 Nov 2004

Workshop

Workshop	NORFA Yeast Systems Biology Workshop
Country/Territory	Denmark
City	Copenhagen
Period	17/11/04 → 21/11/04

Cite this

@conference{4b79c586172a4258b1fac307d0167438,

title = "The free and carrier-bound yeast populations from a two-stage immobilised yeast reactor are in different physiological condition",

abstract = "To better understand the operation of a 2-stage immobilised yeast reactor making beer from wort, different yeast populations from the first stage were analysed. Yeast amounts (mg fresh yeast/g wood chip) were about: free, 10; loosely bound, 78; tightly bound, 40. Proteomics showed that from the 300 most abundant soluble proteins 38 had at least 2-fold different abundancies in free and loosely bound yeast. Some of these variable proteins have been identified. Loosely bound yeast contained less sterol and less unsaturated fatty acids than free yeast. Maltose permease activity was two-fold greater in free than loosely bound yeast.",

author = "Virve Vidgren and Anne Huuskonen and Ilkka Virkaj{\"a}rvi and Heini Koivistoinen and John Londesborough",

year = "2004",

language = "English",

note = "NORFA Yeast Systems Biology Workshop ; Conference date: 17-11-2004 Through 21-11-2004",

}

TY - CONF

T1 - The free and carrier-bound yeast populations from a two-stage immobilised yeast reactor are in different physiological condition

AU - Vidgren, Virve

AU - Huuskonen, Anne

AU - Virkajärvi, Ilkka

AU - Koivistoinen, Heini

AU - Londesborough, John

PY - 2004

Y1 - 2004

N2 - To better understand the operation of a 2-stage immobilised yeast reactor making beer from wort, different yeast populations from the first stage were analysed. Yeast amounts (mg fresh yeast/g wood chip) were about: free, 10; loosely bound, 78; tightly bound, 40. Proteomics showed that from the 300 most abundant soluble proteins 38 had at least 2-fold different abundancies in free and loosely bound yeast. Some of these variable proteins have been identified. Loosely bound yeast contained less sterol and less unsaturated fatty acids than free yeast. Maltose permease activity was two-fold greater in free than loosely bound yeast.

AB - To better understand the operation of a 2-stage immobilised yeast reactor making beer from wort, different yeast populations from the first stage were analysed. Yeast amounts (mg fresh yeast/g wood chip) were about: free, 10; loosely bound, 78; tightly bound, 40. Proteomics showed that from the 300 most abundant soluble proteins 38 had at least 2-fold different abundancies in free and loosely bound yeast. Some of these variable proteins have been identified. Loosely bound yeast contained less sterol and less unsaturated fatty acids than free yeast. Maltose permease activity was two-fold greater in free than loosely bound yeast.

M3 - Conference article

T2 - NORFA Yeast Systems Biology Workshop

Y2 - 17 November 2004 through 21 November 2004

ER -

The free and carrier-bound yeast populations from a two-stage immobilised yeast reactor are in different physiological condition

Abstract

Workshop

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