The free and carrier-bound yeast populations from a two-stage immobilized yeast reactor are in different physiological conditions

Virve Vidgren; Ilkka Virkajärvi; Laura Ruohonen; Laura Salusjärvi; John Londesborough

The free and carrier-bound yeast populations from a two-stage immobilized yeast reactor are in different physiological conditions

Virve Vidgren
, Ilkka Virkajärvi
, Laura Ruohonen
, Laura Salusjärvi
, John Londesborough

VTT (former employee or external)

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

Abstract

To better understand the operation of a 2-stage immobilised yeast reactor making beer from wort, different yeast populations from the first stage were analysed. Yeast amounts (mg fresh yeast/g wood chip) were about: free, 10; loosely bound, 78; tightly bound, 40. Proteomics showed that from the 200 most abundant soluble proteins only 19 were present at different levels (up to 12-fold) in free and loosely bound yeast. Maltose permease activity was two-fold greater in free than loosely bound yeast. Other differences will be reported.

Original language	English
Title of host publication	Proceedings of the 29th EBC Congress, Dublin 2003
Publisher	Fachverlag Hans Carl
Pages	609-617
ISBN (Print)	90-70143-22-4
Publication status	Published - 2003
MoE publication type	A4 Article in a conference publication
Event	29th European Brewery Convention EBC Congress 2003 - Dublin, Ireland Duration: 17 May 2003 → 22 May 2003

Conference

Conference	29th European Brewery Convention EBC Congress 2003
Country/Territory	Ireland
City	Dublin
Period	17/05/03 → 22/05/03

Keywords

enzymic activity
immobilized yeast
maltose permease
physiology
yeast analysis

Cite this

@inproceedings{a19c1b54e3934c0591d1ec0cda034f18,

title = "The free and carrier-bound yeast populations from a two-stage immobilized yeast reactor are in different physiological conditions",

abstract = "To better understand the operation of a 2-stage immobilised yeast reactor making beer from wort, different yeast populations from the first stage were analysed. Yeast amounts (mg fresh yeast/g wood chip) were about: free, 10; loosely bound, 78; tightly bound, 40. Proteomics showed that from the 200 most abundant soluble proteins only 19 were present at different levels (up to 12-fold) in free and loosely bound yeast. Maltose permease activity was two-fold greater in free than loosely bound yeast. Other differences will be reported.",

keywords = "enzymic activity, immobilized yeast, maltose permease, physiology, yeast analysis",

author = "Virve Vidgren and Ilkka Virkaj{\"a}rvi and Laura Ruohonen and Laura Salusj{\"a}rvi and John Londesborough",

note = "CD-ROM; 29th European Brewery Convention EBC Congress 2003 ; Conference date: 17-05-2003 Through 22-05-2003",

year = "2003",

language = "English",

isbn = "90-70143-22-4",

pages = "609--617",

booktitle = "Proceedings of the 29th EBC Congress, Dublin 2003",

publisher = "Fachverlag Hans Carl",

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}

Vidgren, V, Virkajärvi, I, Ruohonen, L, Salusjärvi, L & Londesborough, J 2003, The free and carrier-bound yeast populations from a two-stage immobilized yeast reactor are in different physiological conditions. in Proceedings of the 29th EBC Congress, Dublin 2003. Fachverlag Hans Carl, pp. 609-617, 29th European Brewery Convention EBC Congress 2003, Dublin, Ireland, 17/05/03.

The free and carrier-bound yeast populations from a two-stage immobilized yeast reactor are in different physiological conditions. / Vidgren, Virve; Virkajärvi, Ilkka; Ruohonen, Laura et al.
Proceedings of the 29th EBC Congress, Dublin 2003. Fachverlag Hans Carl, 2003. p. 609-617.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

TY - GEN

T1 - The free and carrier-bound yeast populations from a two-stage immobilized yeast reactor are in different physiological conditions

AU - Vidgren, Virve

AU - Virkajärvi, Ilkka

AU - Ruohonen, Laura

AU - Salusjärvi, Laura

AU - Londesborough, John

N1 - CD-ROM

PY - 2003

Y1 - 2003

N2 - To better understand the operation of a 2-stage immobilised yeast reactor making beer from wort, different yeast populations from the first stage were analysed. Yeast amounts (mg fresh yeast/g wood chip) were about: free, 10; loosely bound, 78; tightly bound, 40. Proteomics showed that from the 200 most abundant soluble proteins only 19 were present at different levels (up to 12-fold) in free and loosely bound yeast. Maltose permease activity was two-fold greater in free than loosely bound yeast. Other differences will be reported.

AB - To better understand the operation of a 2-stage immobilised yeast reactor making beer from wort, different yeast populations from the first stage were analysed. Yeast amounts (mg fresh yeast/g wood chip) were about: free, 10; loosely bound, 78; tightly bound, 40. Proteomics showed that from the 200 most abundant soluble proteins only 19 were present at different levels (up to 12-fold) in free and loosely bound yeast. Maltose permease activity was two-fold greater in free than loosely bound yeast. Other differences will be reported.

KW - enzymic activity

KW - immobilized yeast

KW - maltose permease

KW - physiology

KW - yeast analysis

M3 - Conference article in proceedings

SN - 90-70143-22-4

SP - 609

EP - 617

BT - Proceedings of the 29th EBC Congress, Dublin 2003

PB - Fachverlag Hans Carl

T2 - 29th European Brewery Convention EBC Congress 2003

Y2 - 17 May 2003 through 22 May 2003

ER -

The free and carrier-bound yeast populations from a two-stage immobilized yeast reactor are in different physiological conditions

Abstract

Conference

Keywords

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