The function of Sec1/Munc18 proteins - Solution of the mystery in sight?

Maria Kauppi, Jussi Jäntti, Vesa M. Olkkonen

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

Abstract

Members of the Secl/Munc18 (SM) protein family form a central part of the machinery responsible for transport vesicle docking and fusion. Their hallmark property is interaction with SNARE proteins belonging to the syntaxin family. A wealth of evidence proposes an essential positive role for SM proteins in the different intracellular trafficking events in all cell types and organisms studied. However, the exact nature of their function is not understood. The existing data suggests functions as chaperones of syntaxins, in transport vesicle docking, promotion of SNARE complex formation, and in fusion pore opening. Intriguingly, all SM proteins do not bind their cognate syntaxin partners in a similar manner, but four different binding modes can be distinguished. This suggests that the unifying factor in SM function is not an intimate tight-fitting contact with syntaxins. We propose here a model, based on increasing experimental evidence that the key function of SM proteins is to bridge the Rab-GTPase dependent machineries of transport vesicle tethering and the SNARE apparatus responsible for vesicle docking and fusion.
Original languageEnglish
Title of host publicationRegulatory Mechanisms of Intracellular Membrane Transport. Keränen, S.; Jäntti, J. (Eds). Topics in Current Genetics : 10
EditorsSirkka Keränen, Jussi Jäntti
Place of PublicationBerlin - Heidelberg - New York
PublisherSpringer
Pages115-143
ISBN (Electronic)978-3-540-44476-3
ISBN (Print)978-3-540-22302-3
DOIs
Publication statusPublished - 2004
MoE publication typeA3 Part of a book or another research book

Publication series

NameTopics in Current Genetics
PublisherSpringer
Volume10
ISSN (Print)1610-2096
ISSN (Electronic)1610-6970

Fingerprint

Munc18 Proteins
Qa-SNARE Proteins
SNARE Proteins
Transport Vesicles
rab GTP-Binding Proteins

Keywords

  • proteins
  • SNARE proteins
  • transport vesicle docking
  • syntaxin

Cite this

Kauppi, M., Jäntti, J., & Olkkonen, V. M. (2004). The function of Sec1/Munc18 proteins - Solution of the mystery in sight? In S. Keränen, & J. Jäntti (Eds.), Regulatory Mechanisms of Intracellular Membrane Transport. Keränen, S.; Jäntti, J. (Eds). Topics in Current Genetics : 10 (pp. 115-143). Berlin - Heidelberg - New York: Springer. Topics in Current Genetics, Vol.. 10 https://doi.org/10.1007/b97172
Kauppi, Maria ; Jäntti, Jussi ; Olkkonen, Vesa M. / The function of Sec1/Munc18 proteins - Solution of the mystery in sight?. Regulatory Mechanisms of Intracellular Membrane Transport. Keränen, S.; Jäntti, J. (Eds). Topics in Current Genetics : 10. editor / Sirkka Keränen ; Jussi Jäntti. Berlin - Heidelberg - New York : Springer, 2004. pp. 115-143 (Topics in Current Genetics, Vol. 10).
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Kauppi, M, Jäntti, J & Olkkonen, VM 2004, The function of Sec1/Munc18 proteins - Solution of the mystery in sight? in S Keränen & J Jäntti (eds), Regulatory Mechanisms of Intracellular Membrane Transport. Keränen, S.; Jäntti, J. (Eds). Topics in Current Genetics : 10. Springer, Berlin - Heidelberg - New York, Topics in Current Genetics, vol. 10, pp. 115-143. https://doi.org/10.1007/b97172

The function of Sec1/Munc18 proteins - Solution of the mystery in sight? / Kauppi, Maria; Jäntti, Jussi; Olkkonen, Vesa M.

Regulatory Mechanisms of Intracellular Membrane Transport. Keränen, S.; Jäntti, J. (Eds). Topics in Current Genetics : 10. ed. / Sirkka Keränen; Jussi Jäntti. Berlin - Heidelberg - New York : Springer, 2004. p. 115-143 (Topics in Current Genetics, Vol. 10).

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

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T1 - The function of Sec1/Munc18 proteins - Solution of the mystery in sight?

AU - Kauppi, Maria

AU - Jäntti, Jussi

AU - Olkkonen, Vesa M.

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N2 - Members of the Secl/Munc18 (SM) protein family form a central part of the machinery responsible for transport vesicle docking and fusion. Their hallmark property is interaction with SNARE proteins belonging to the syntaxin family. A wealth of evidence proposes an essential positive role for SM proteins in the different intracellular trafficking events in all cell types and organisms studied. However, the exact nature of their function is not understood. The existing data suggests functions as chaperones of syntaxins, in transport vesicle docking, promotion of SNARE complex formation, and in fusion pore opening. Intriguingly, all SM proteins do not bind their cognate syntaxin partners in a similar manner, but four different binding modes can be distinguished. This suggests that the unifying factor in SM function is not an intimate tight-fitting contact with syntaxins. We propose here a model, based on increasing experimental evidence that the key function of SM proteins is to bridge the Rab-GTPase dependent machineries of transport vesicle tethering and the SNARE apparatus responsible for vesicle docking and fusion.

AB - Members of the Secl/Munc18 (SM) protein family form a central part of the machinery responsible for transport vesicle docking and fusion. Their hallmark property is interaction with SNARE proteins belonging to the syntaxin family. A wealth of evidence proposes an essential positive role for SM proteins in the different intracellular trafficking events in all cell types and organisms studied. However, the exact nature of their function is not understood. The existing data suggests functions as chaperones of syntaxins, in transport vesicle docking, promotion of SNARE complex formation, and in fusion pore opening. Intriguingly, all SM proteins do not bind their cognate syntaxin partners in a similar manner, but four different binding modes can be distinguished. This suggests that the unifying factor in SM function is not an intimate tight-fitting contact with syntaxins. We propose here a model, based on increasing experimental evidence that the key function of SM proteins is to bridge the Rab-GTPase dependent machineries of transport vesicle tethering and the SNARE apparatus responsible for vesicle docking and fusion.

KW - proteins

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BT - Regulatory Mechanisms of Intracellular Membrane Transport. Keränen, S.; Jäntti, J. (Eds). Topics in Current Genetics : 10

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A2 - Jäntti, Jussi

PB - Springer

CY - Berlin - Heidelberg - New York

ER -

Kauppi M, Jäntti J, Olkkonen VM. The function of Sec1/Munc18 proteins - Solution of the mystery in sight? In Keränen S, Jäntti J, editors, Regulatory Mechanisms of Intracellular Membrane Transport. Keränen, S.; Jäntti, J. (Eds). Topics in Current Genetics : 10. Berlin - Heidelberg - New York: Springer. 2004. p. 115-143. (Topics in Current Genetics, Vol. 10). https://doi.org/10.1007/b97172