The fungal path for D-galacturonate catabolism

Satu Hilditch, Suvi Berghäll, Janis Liepins, Merja Penttilä, Peter Richard

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

D-galacturonate is the major component of pectin and consequently an important carbon source for microorganisms living on decaying plant material or for biotechnological processes where cheap raw materials such as sugar beet pulp are used. A bacterial catabolic pathway has been described while a eukaryotic pathway has remained unknown. For E. coli a pathway was described consisting of five enzymes converting D-galacturonate to pyruvate and D-glyceraldehyde-3-phosphate. The enzymes of this pathway are uronate isomerase, NADH-utilizing D-tagaturonate reductase, altronate dehydratase, D-erythro-3-deoxy-hexulosonate kinase and D-erythro-3-deoxy-hexulosonate-6-phosphate aldolase. We show that the D-galacturonate pathway is different in the mold Hypocrea jecorina (Trichoderma reesei). In this fungal catabolic pathway D-galacturonate is converted to pyruvate and glycerol. The intermediates are L-galactonate, L-threo-3-deoxy-hexulosonate and L-glyceraldehyde. The pathway contains four enzymes, NADPH-utilizing D-galacturonate reductase, L-galactonate dehydratase, L-threo-3-deoxy-hexulosonate aldolase and a glycerol dehydrogenase that converts L-glyceraldehyde to glycerol in the reverse reaction. All the corresponding genes have been identified, cloned, and expressed in the heterologous host S. cerevisiae, and the kinetic properties of the enzymes have been determined.
Original languageEnglish
Title of host publication3rd European Federation of Biotechnology Conference
Subtitle of host publicationPhysiology of Yeasts and Filamentous Fungi PYFF3
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages123
ISBN (Electronic)978-951-38-6314-2
ISBN (Print)978-951-38-6313-5
Publication statusPublished - 2007
Event3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi - Helsinki, Finland
Duration: 13 Jun 200716 Jun 2007

Publication series

SeriesVTT Symposium
Number245
ISSN0357-9387

Conference

Conference3rd European Federation of Biotechnology Conference
Abbreviated titlePYFF3
CountryFinland
CityHelsinki
Period13/06/0716/06/07

Fingerprint

glyceraldehyde
Trichoderma reesei
fructose-bisphosphate aldolase
metabolism
enzymes
glycerol
glycerol dehydrogenase
glyceraldehyde 3-phosphate
sugar beet pulp
NAD (coenzyme)
isomerases
NADP (coenzyme)
molds (fungi)
pectins
raw materials
phosphotransferases (kinases)
phosphates
Escherichia coli
microorganisms
kinetics

Cite this

Hilditch, S., Berghäll, S., Liepins, J., Penttilä, M., & Richard, P. (2007). The fungal path for D-galacturonate catabolism. In 3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi PYFF3 (pp. 123). [P69] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 245
Hilditch, Satu ; Berghäll, Suvi ; Liepins, Janis ; Penttilä, Merja ; Richard, Peter. / The fungal path for D-galacturonate catabolism. 3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo : VTT Technical Research Centre of Finland, 2007. pp. 123 (VTT Symposium; No. 245).
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Hilditch, S, Berghäll, S, Liepins, J, Penttilä, M & Richard, P 2007, The fungal path for D-galacturonate catabolism. in 3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi PYFF3., P69, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 245, pp. 123, 3rd European Federation of Biotechnology Conference , Helsinki, Finland, 13/06/07.

The fungal path for D-galacturonate catabolism. / Hilditch, Satu; Berghäll, Suvi; Liepins, Janis; Penttilä, Merja; Richard, Peter.

3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo : VTT Technical Research Centre of Finland, 2007. p. 123 P69 (VTT Symposium; No. 245).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - The fungal path for D-galacturonate catabolism

AU - Hilditch, Satu

AU - Berghäll, Suvi

AU - Liepins, Janis

AU - Penttilä, Merja

AU - Richard, Peter

PY - 2007

Y1 - 2007

N2 - D-galacturonate is the major component of pectin and consequently an important carbon source for microorganisms living on decaying plant material or for biotechnological processes where cheap raw materials such as sugar beet pulp are used. A bacterial catabolic pathway has been described while a eukaryotic pathway has remained unknown. For E. coli a pathway was described consisting of five enzymes converting D-galacturonate to pyruvate and D-glyceraldehyde-3-phosphate. The enzymes of this pathway are uronate isomerase, NADH-utilizing D-tagaturonate reductase, altronate dehydratase, D-erythro-3-deoxy-hexulosonate kinase and D-erythro-3-deoxy-hexulosonate-6-phosphate aldolase. We show that the D-galacturonate pathway is different in the mold Hypocrea jecorina (Trichoderma reesei). In this fungal catabolic pathway D-galacturonate is converted to pyruvate and glycerol. The intermediates are L-galactonate, L-threo-3-deoxy-hexulosonate and L-glyceraldehyde. The pathway contains four enzymes, NADPH-utilizing D-galacturonate reductase, L-galactonate dehydratase, L-threo-3-deoxy-hexulosonate aldolase and a glycerol dehydrogenase that converts L-glyceraldehyde to glycerol in the reverse reaction. All the corresponding genes have been identified, cloned, and expressed in the heterologous host S. cerevisiae, and the kinetic properties of the enzymes have been determined.

AB - D-galacturonate is the major component of pectin and consequently an important carbon source for microorganisms living on decaying plant material or for biotechnological processes where cheap raw materials such as sugar beet pulp are used. A bacterial catabolic pathway has been described while a eukaryotic pathway has remained unknown. For E. coli a pathway was described consisting of five enzymes converting D-galacturonate to pyruvate and D-glyceraldehyde-3-phosphate. The enzymes of this pathway are uronate isomerase, NADH-utilizing D-tagaturonate reductase, altronate dehydratase, D-erythro-3-deoxy-hexulosonate kinase and D-erythro-3-deoxy-hexulosonate-6-phosphate aldolase. We show that the D-galacturonate pathway is different in the mold Hypocrea jecorina (Trichoderma reesei). In this fungal catabolic pathway D-galacturonate is converted to pyruvate and glycerol. The intermediates are L-galactonate, L-threo-3-deoxy-hexulosonate and L-glyceraldehyde. The pathway contains four enzymes, NADPH-utilizing D-galacturonate reductase, L-galactonate dehydratase, L-threo-3-deoxy-hexulosonate aldolase and a glycerol dehydrogenase that converts L-glyceraldehyde to glycerol in the reverse reaction. All the corresponding genes have been identified, cloned, and expressed in the heterologous host S. cerevisiae, and the kinetic properties of the enzymes have been determined.

M3 - Conference abstract in proceedings

SN - 978-951-38-6313-5

T3 - VTT Symposium

SP - 123

BT - 3rd European Federation of Biotechnology Conference

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Hilditch S, Berghäll S, Liepins J, Penttilä M, Richard P. The fungal path for D-galacturonate catabolism. In 3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo: VTT Technical Research Centre of Finland. 2007. p. 123. P69. (VTT Symposium; No. 245).