The fungal path for D-galacturonate catabolism

Satu Hilditch, Suvi Berghäll, Janis Liepins, Merja Penttilä, Peter Richard

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

    Abstract

    D-galacturonate is the major component of pectin and consequently an important carbon source for microorganisms living on decaying plant material or for biotechnological processes where cheap raw materials such as sugar beet pulp are used. A bacterial catabolic pathway has been described while a eukaryotic pathway has remained unknown. For E. coli a pathway was described consisting of five enzymes converting D-galacturonate to pyruvate and D-glyceraldehyde-3-phosphate. The enzymes of this pathway are uronate isomerase, NADH-utilizing D-tagaturonate reductase, altronate dehydratase, D-erythro-3-deoxy-hexulosonate kinase and D-erythro-3-deoxy-hexulosonate-6-phosphate aldolase. We show that the D-galacturonate pathway is different in the mold Hypocrea jecorina (Trichoderma reesei). In this fungal catabolic pathway D-galacturonate is converted to pyruvate and glycerol. The intermediates are L-galactonate, L-threo-3-deoxy-hexulosonate and L-glyceraldehyde. The pathway contains four enzymes, NADPH-utilizing D-galacturonate reductase, L-galactonate dehydratase, L-threo-3-deoxy-hexulosonate aldolase and a glycerol dehydrogenase that converts L-glyceraldehyde to glycerol in the reverse reaction. All the corresponding genes have been identified, cloned, and expressed in the heterologous host S. cerevisiae, and the kinetic properties of the enzymes have been determined.
    Original languageEnglish
    Title of host publication3rd European Federation of Biotechnology Conference
    Subtitle of host publicationPhysiology of Yeasts and Filamentous Fungi PYFF3
    Place of PublicationEspoo
    PublisherVTT Technical Research Centre of Finland
    Pages123
    ISBN (Electronic)978-951-38-6314-2
    ISBN (Print)978-951-38-6313-5
    Publication statusPublished - 2007
    Event3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi - Helsinki, Finland
    Duration: 13 Jun 200716 Jun 2007

    Publication series

    SeriesVTT Symposium
    Number245
    ISSN0357-9387

    Conference

    Conference3rd European Federation of Biotechnology Conference
    Abbreviated titlePYFF3
    Country/TerritoryFinland
    CityHelsinki
    Period13/06/0716/06/07

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