Abstract
| Original language | English |
|---|---|
| Title of host publication | International Specialised Symposium on Yeasts ISSY25 |
| Subtitle of host publication | Systems Biology of Yeasts - from Models to Applications |
| Place of Publication | Espoo |
| Publisher | VTT Technical Research Centre of Finland |
| Pages | 142 |
| ISBN (Electronic) | 951-38-6308-5 |
| ISBN (Print) | 951-38-6307-7 |
| Publication status | Published - 2006 |
| Event | International Specialised Symposium on Yeasts, ISSY 25 - Espoo, Finland Duration: 18 Jun 2006 → 21 Jun 2006 |
Publication series
| Series | VTT Symposium |
|---|---|
| Number | 242 |
| ISSN | 0357-9387 |
Conference
| Conference | International Specialised Symposium on Yeasts, ISSY 25 |
|---|---|
| Abbreviated title | ISSY 25 |
| Country | Finland |
| City | Espoo |
| Period | 18/06/06 → 21/06/06 |
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The fungal path for D-galacturonic acid catabolism. / Kuorelahti, Satu; Penttilä, Merja; Richard, Peter.
International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications. Espoo : VTT Technical Research Centre of Finland, 2006. p. 142 P86 (VTT Symposium; No. 242).Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Scientific
TY - CHAP
T1 - The fungal path for D-galacturonic acid catabolism
AU - Kuorelahti, Satu
AU - Penttilä, Merja
AU - Richard, Peter
PY - 2006
Y1 - 2006
N2 - D-galacturonic acid is the major component of pectin and consequently an important carbon source for microorganisms living on decaying plant material or for biotechnological processes where cheap raw materials such as sugar beet pulp are used. A bacterial catabolic pathway has been described while a eukaryotic pathway has remained unknown. For E. coli a pathway was described consisting of five enzymes converting D-galacturonic acid to pyruvic acid and D-glyceraldehyde-3-phosphate. The enzymes of this pathway are uronate isomerase, NADH-utilizing D-tagaturonate reductase, altronate dehydratase, D-erythro-3-deoxy-D-hexulosonate kinase and D-erythro-3-deoxy-D-hexulosonate-6-phosphate aldolase. We show that a fungal pathway exists that is distinctly different from any previously described pathway. In this pathway D-galacturonic acid is converted to pyruvate and glycerol. Intermediates are L-galactonate and L-threo-3-deoxy-hexulosonate. We have identified most of the enzymes of this pathway, cloned the corresponding genes, expressed them in a heterologus host and determined the kinetic properties of the enzymes. We will present potential biotechnological applications of this novel pathway.
AB - D-galacturonic acid is the major component of pectin and consequently an important carbon source for microorganisms living on decaying plant material or for biotechnological processes where cheap raw materials such as sugar beet pulp are used. A bacterial catabolic pathway has been described while a eukaryotic pathway has remained unknown. For E. coli a pathway was described consisting of five enzymes converting D-galacturonic acid to pyruvic acid and D-glyceraldehyde-3-phosphate. The enzymes of this pathway are uronate isomerase, NADH-utilizing D-tagaturonate reductase, altronate dehydratase, D-erythro-3-deoxy-D-hexulosonate kinase and D-erythro-3-deoxy-D-hexulosonate-6-phosphate aldolase. We show that a fungal pathway exists that is distinctly different from any previously described pathway. In this pathway D-galacturonic acid is converted to pyruvate and glycerol. Intermediates are L-galactonate and L-threo-3-deoxy-hexulosonate. We have identified most of the enzymes of this pathway, cloned the corresponding genes, expressed them in a heterologus host and determined the kinetic properties of the enzymes. We will present potential biotechnological applications of this novel pathway.
M3 - Conference abstract in proceedings
SN - 951-38-6307-7
T3 - VTT Symposium
SP - 142
BT - International Specialised Symposium on Yeasts ISSY25
PB - VTT Technical Research Centre of Finland
CY - Espoo
ER -