The fungal path for D-galacturonic acid catabolism

Satu Kuorelahti, Merja Penttilä, Peter Richard

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

D-galacturonic acid is the major component of pectin and consequently an important carbon source for microorganisms living on decaying plant material or for biotechnological processes where cheap raw materials such as sugar beet pulp are used. A bacterial catabolic pathway has been described while a eukaryotic pathway has remained unknown. For E. coli a pathway was described consisting of five enzymes converting D-galacturonic acid to pyruvic acid and D-glyceraldehyde-3-phosphate. The enzymes of this pathway are uronate isomerase, NADH-utilizing D-tagaturonate reductase, altronate dehydratase, D-erythro-3-deoxy-D-hexulosonate kinase and D-erythro-3-deoxy-D-hexulosonate-6-phosphate aldolase. We show that a fungal pathway exists that is distinctly different from any previously described pathway. In this pathway D-galacturonic acid is converted to pyruvate and glycerol. Intermediates are L-galactonate and L-threo-3-deoxy-hexulosonate. We have identified most of the enzymes of this pathway, cloned the corresponding genes, expressed them in a heterologus host and determined the kinetic properties of the enzymes. We will present potential biotechnological applications of this novel pathway.
Original languageEnglish
Title of host publicationInternational Specialised Symposium on Yeasts ISSY25
Subtitle of host publicationSystems Biology of Yeasts - from Models to Applications
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages142
ISBN (Electronic)951-38-6308-5
ISBN (Print)951-38-6307-7
Publication statusPublished - 2006
EventInternational Specialised Symposium on Yeasts, ISSY 25 - Espoo, Finland
Duration: 18 Jun 200621 Jun 2006

Publication series

SeriesVTT Symposium
Number242
ISSN0357-9387

Conference

ConferenceInternational Specialised Symposium on Yeasts, ISSY 25
Abbreviated titleISSY 25
CountryFinland
CityEspoo
Period18/06/0621/06/06

Fingerprint

galacturonic acid
metabolism
enzymes
glyceraldehyde 3-phosphate
pyruvic acid
fructose-bisphosphate aldolase
sugar beet pulp
isomerases
pectins
raw materials
glycerol
phosphotransferases (kinases)
phosphates
Escherichia coli
microorganisms
kinetics
carbon
genes

Cite this

Kuorelahti, S., Penttilä, M., & Richard, P. (2006). The fungal path for D-galacturonic acid catabolism. In International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications (pp. 142). [P86] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 242
Kuorelahti, Satu ; Penttilä, Merja ; Richard, Peter. / The fungal path for D-galacturonic acid catabolism. International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications. Espoo : VTT Technical Research Centre of Finland, 2006. pp. 142 (VTT Symposium; No. 242).
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Kuorelahti, S, Penttilä, M & Richard, P 2006, The fungal path for D-galacturonic acid catabolism. in International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications., P86, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 242, pp. 142, International Specialised Symposium on Yeasts, ISSY 25 , Espoo, Finland, 18/06/06.

The fungal path for D-galacturonic acid catabolism. / Kuorelahti, Satu; Penttilä, Merja; Richard, Peter.

International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications. Espoo : VTT Technical Research Centre of Finland, 2006. p. 142 P86 (VTT Symposium; No. 242).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - The fungal path for D-galacturonic acid catabolism

AU - Kuorelahti, Satu

AU - Penttilä, Merja

AU - Richard, Peter

PY - 2006

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N2 - D-galacturonic acid is the major component of pectin and consequently an important carbon source for microorganisms living on decaying plant material or for biotechnological processes where cheap raw materials such as sugar beet pulp are used. A bacterial catabolic pathway has been described while a eukaryotic pathway has remained unknown. For E. coli a pathway was described consisting of five enzymes converting D-galacturonic acid to pyruvic acid and D-glyceraldehyde-3-phosphate. The enzymes of this pathway are uronate isomerase, NADH-utilizing D-tagaturonate reductase, altronate dehydratase, D-erythro-3-deoxy-D-hexulosonate kinase and D-erythro-3-deoxy-D-hexulosonate-6-phosphate aldolase. We show that a fungal pathway exists that is distinctly different from any previously described pathway. In this pathway D-galacturonic acid is converted to pyruvate and glycerol. Intermediates are L-galactonate and L-threo-3-deoxy-hexulosonate. We have identified most of the enzymes of this pathway, cloned the corresponding genes, expressed them in a heterologus host and determined the kinetic properties of the enzymes. We will present potential biotechnological applications of this novel pathway.

AB - D-galacturonic acid is the major component of pectin and consequently an important carbon source for microorganisms living on decaying plant material or for biotechnological processes where cheap raw materials such as sugar beet pulp are used. A bacterial catabolic pathway has been described while a eukaryotic pathway has remained unknown. For E. coli a pathway was described consisting of five enzymes converting D-galacturonic acid to pyruvic acid and D-glyceraldehyde-3-phosphate. The enzymes of this pathway are uronate isomerase, NADH-utilizing D-tagaturonate reductase, altronate dehydratase, D-erythro-3-deoxy-D-hexulosonate kinase and D-erythro-3-deoxy-D-hexulosonate-6-phosphate aldolase. We show that a fungal pathway exists that is distinctly different from any previously described pathway. In this pathway D-galacturonic acid is converted to pyruvate and glycerol. Intermediates are L-galactonate and L-threo-3-deoxy-hexulosonate. We have identified most of the enzymes of this pathway, cloned the corresponding genes, expressed them in a heterologus host and determined the kinetic properties of the enzymes. We will present potential biotechnological applications of this novel pathway.

M3 - Conference abstract in proceedings

SN - 951-38-6307-7

T3 - VTT Symposium

SP - 142

BT - International Specialised Symposium on Yeasts ISSY25

PB - VTT Technical Research Centre of Finland

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ER -

Kuorelahti S, Penttilä M, Richard P. The fungal path for D-galacturonic acid catabolism. In International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications. Espoo: VTT Technical Research Centre of Finland. 2006. p. 142. P86. (VTT Symposium; No. 242).