Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 897-902 |
| Number of pages | 6 |
| Journal | Yeast |
| Volume | 18 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2001 |
| MoE publication type | A1 Journal article-refereed |
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The GDII genes from Kluyveromyces lactis and Pichia pastoris : Cloning and functional expression in Saccharomyces cerevisiae. / Brummer, Mirko; Richard, Peter; Sundqvist, Lena; Väänänen, Ritva; Keränen, Sirkka (Corresponding Author).
In: Yeast, Vol. 18, No. 10, 2001, p. 897-902.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - The GDII genes from Kluyveromyces lactis and Pichia pastoris
T2 - Cloning and functional expression in Saccharomyces cerevisiae
AU - Brummer, Mirko
AU - Richard, Peter
AU - Sundqvist, Lena
AU - Väänänen, Ritva
AU - Keränen, Sirkka
PY - 2001
Y1 - 2001
N2 - The nucleotide sequences of 2.8 kb and 2.9 kb fragments containing the Kluyveromyces lactis and Pichia pastoris GDI1 genes, respectively, were determined. K. lactis GDI1 was found during sequencing of a genomic library clone, whereas the P. pastoris GDI1 was obtained from a genomic library by complementing a Saccharomyces cerevisiae sec19‐1 mutant strain. The sequenced DNA fragments contain open reading frames of 1338 bp (K.lactis) and 1344 bp (P. pastoris), coding for polypeptides of 445 and 447 residues, respectively. Both sequences fully complement the S. cerevisiae sec19‐1 mutation. They have high degrees of homology with known GDP dissociation inhibitors from yeast species and other eukaryotes. The GenBank Accession Nos of the sequences are AF255332 (K.lactis GDI1) and AY007574 (P. pastoris GDI1).
AB - The nucleotide sequences of 2.8 kb and 2.9 kb fragments containing the Kluyveromyces lactis and Pichia pastoris GDI1 genes, respectively, were determined. K. lactis GDI1 was found during sequencing of a genomic library clone, whereas the P. pastoris GDI1 was obtained from a genomic library by complementing a Saccharomyces cerevisiae sec19‐1 mutant strain. The sequenced DNA fragments contain open reading frames of 1338 bp (K.lactis) and 1344 bp (P. pastoris), coding for polypeptides of 445 and 447 residues, respectively. Both sequences fully complement the S. cerevisiae sec19‐1 mutation. They have high degrees of homology with known GDP dissociation inhibitors from yeast species and other eukaryotes. The GenBank Accession Nos of the sequences are AF255332 (K.lactis GDI1) and AY007574 (P. pastoris GDI1).
U2 - 10.1002/yea.736
DO - 10.1002/yea.736
M3 - Article
VL - 18
SP - 897
EP - 902
JO - Yeast
JF - Yeast
SN - 0749-503X
IS - 10
ER -