The GDII genes from Kluyveromyces lactis and Pichia pastoris

Cloning and functional expression in Saccharomyces cerevisiae

Mirko Brummer, Peter Richard, Lena Sundqvist, Ritva Väänänen, Sirkka Keränen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)

Abstract

The nucleotide sequences of 2.8 kb and 2.9 kb fragments containing the Kluyveromyces lactis and Pichia pastoris GDI1 genes, respectively, were determined. K. lactis GDI1 was found during sequencing of a genomic library clone, whereas the P. pastoris GDI1 was obtained from a genomic library by complementing a Saccharomyces cerevisiae sec19‐1 mutant strain. The sequenced DNA fragments contain open reading frames of 1338 bp (K.lactis) and 1344 bp (P. pastoris), coding for polypeptides of 445 and 447 residues, respectively. Both sequences fully complement the S. cerevisiae sec19‐1 mutation. They have high degrees of homology with known GDP dissociation inhibitors from yeast species and other eukaryotes. The GenBank Accession Nos of the sequences are AF255332 (K.lactis GDI1) and AY007574 (P. pastoris GDI1).
Original languageEnglish
Pages (from-to)897-902
Number of pages6
JournalYeast
Volume18
Issue number10
DOIs
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

Fingerprint

Kluyveromyces
Pichia
Genomic Library
Cloning
Yeast
Saccharomyces cerevisiae
Organism Cloning
Genes
Guanine Nucleotide Dissociation Inhibitors
Nucleic Acid Databases
Eukaryota
Open Reading Frames
Clone Cells
Yeasts
Polypeptides
Nucleotides
Peptides
Mutation
DNA

Cite this

Brummer, Mirko ; Richard, Peter ; Sundqvist, Lena ; Väänänen, Ritva ; Keränen, Sirkka. / The GDII genes from Kluyveromyces lactis and Pichia pastoris : Cloning and functional expression in Saccharomyces cerevisiae. In: Yeast. 2001 ; Vol. 18, No. 10. pp. 897-902.
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abstract = "The nucleotide sequences of 2.8 kb and 2.9 kb fragments containing the Kluyveromyces lactis and Pichia pastoris GDI1 genes, respectively, were determined. K. lactis GDI1 was found during sequencing of a genomic library clone, whereas the P. pastoris GDI1 was obtained from a genomic library by complementing a Saccharomyces cerevisiae sec19‐1 mutant strain. The sequenced DNA fragments contain open reading frames of 1338 bp (K.lactis) and 1344 bp (P. pastoris), coding for polypeptides of 445 and 447 residues, respectively. Both sequences fully complement the S. cerevisiae sec19‐1 mutation. They have high degrees of homology with known GDP dissociation inhibitors from yeast species and other eukaryotes. The GenBank Accession Nos of the sequences are AF255332 (K.lactis GDI1) and AY007574 (P. pastoris GDI1).",
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The GDII genes from Kluyveromyces lactis and Pichia pastoris : Cloning and functional expression in Saccharomyces cerevisiae. / Brummer, Mirko; Richard, Peter; Sundqvist, Lena; Väänänen, Ritva; Keränen, Sirkka (Corresponding Author).

In: Yeast, Vol. 18, No. 10, 2001, p. 897-902.

Research output: Contribution to journalArticleScientificpeer-review

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T2 - Cloning and functional expression in Saccharomyces cerevisiae

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AU - Keränen, Sirkka

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AB - The nucleotide sequences of 2.8 kb and 2.9 kb fragments containing the Kluyveromyces lactis and Pichia pastoris GDI1 genes, respectively, were determined. K. lactis GDI1 was found during sequencing of a genomic library clone, whereas the P. pastoris GDI1 was obtained from a genomic library by complementing a Saccharomyces cerevisiae sec19‐1 mutant strain. The sequenced DNA fragments contain open reading frames of 1338 bp (K.lactis) and 1344 bp (P. pastoris), coding for polypeptides of 445 and 447 residues, respectively. Both sequences fully complement the S. cerevisiae sec19‐1 mutation. They have high degrees of homology with known GDP dissociation inhibitors from yeast species and other eukaryotes. The GenBank Accession Nos of the sequences are AF255332 (K.lactis GDI1) and AY007574 (P. pastoris GDI1).

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