The GDII genes from Kluyveromyces lactis and Pichia pastoris: Cloning and functional expression in Saccharomyces cerevisiae

Mirko Brummer; Peter Richard; Lena Sundqvist; Ritva Väänänen; Sirkka Keränen

doi:10.1002/yea.736

The GDII genes from Kluyveromyces lactis and Pichia pastoris: Cloning and functional expression in Saccharomyces cerevisiae

Mirko Brummer, Peter Richard, Lena Sundqvist, Ritva Väänänen, Sirkka Keränen (Corresponding Author)

BA3401 Production host engineering

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Abstract

The nucleotide sequences of 2.8 kb and 2.9 kb fragments containing the Kluyveromyces lactis and Pichia pastoris GDI1 genes, respectively, were determined. K. lactis GDI1 was found during sequencing of a genomic library clone, whereas the P. pastoris GDI1 was obtained from a genomic library by complementing a Saccharomyces cerevisiae sec19‐1 mutant strain. The sequenced DNA fragments contain open reading frames of 1338 bp (K.lactis) and 1344 bp (P. pastoris), coding for polypeptides of 445 and 447 residues, respectively. Both sequences fully complement the S. cerevisiae sec19‐1 mutation. They have high degrees of homology with known GDP dissociation inhibitors from yeast species and other eukaryotes. The GenBank Accession Nos of the sequences are AF255332 (K.lactis GDI1) and AY007574 (P. pastoris GDI1).

Original language	English
Pages (from-to)	897-902
Number of pages	6
Journal	Yeast
Volume	18
Issue number	10
DOIs	https://doi.org/10.1002/yea.736
Publication status	Published - 2001
MoE publication type	A1 Journal article-refereed

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title = "The GDII genes from Kluyveromyces lactis and Pichia pastoris: Cloning and functional expression in Saccharomyces cerevisiae",

abstract = "The nucleotide sequences of 2.8 kb and 2.9 kb fragments containing the Kluyveromyces lactis and Pichia pastoris GDI1 genes, respectively, were determined. K. lactis GDI1 was found during sequencing of a genomic library clone, whereas the P. pastoris GDI1 was obtained from a genomic library by complementing a Saccharomyces cerevisiae sec19‐1 mutant strain. The sequenced DNA fragments contain open reading frames of 1338 bp (K.lactis) and 1344 bp (P. pastoris), coding for polypeptides of 445 and 447 residues, respectively. Both sequences fully complement the S. cerevisiae sec19‐1 mutation. They have high degrees of homology with known GDP dissociation inhibitors from yeast species and other eukaryotes. The GenBank Accession Nos of the sequences are AF255332 (K.lactis GDI1) and AY007574 (P. pastoris GDI1).",

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T1 - The GDII genes from Kluyveromyces lactis and Pichia pastoris

T2 - Cloning and functional expression in Saccharomyces cerevisiae

AU - Brummer, Mirko

AU - Richard, Peter

AU - Sundqvist, Lena

AU - Väänänen, Ritva

AU - Keränen, Sirkka

PY - 2001

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N2 - The nucleotide sequences of 2.8 kb and 2.9 kb fragments containing the Kluyveromyces lactis and Pichia pastoris GDI1 genes, respectively, were determined. K. lactis GDI1 was found during sequencing of a genomic library clone, whereas the P. pastoris GDI1 was obtained from a genomic library by complementing a Saccharomyces cerevisiae sec19‐1 mutant strain. The sequenced DNA fragments contain open reading frames of 1338 bp (K.lactis) and 1344 bp (P. pastoris), coding for polypeptides of 445 and 447 residues, respectively. Both sequences fully complement the S. cerevisiae sec19‐1 mutation. They have high degrees of homology with known GDP dissociation inhibitors from yeast species and other eukaryotes. The GenBank Accession Nos of the sequences are AF255332 (K.lactis GDI1) and AY007574 (P. pastoris GDI1).

AB - The nucleotide sequences of 2.8 kb and 2.9 kb fragments containing the Kluyveromyces lactis and Pichia pastoris GDI1 genes, respectively, were determined. K. lactis GDI1 was found during sequencing of a genomic library clone, whereas the P. pastoris GDI1 was obtained from a genomic library by complementing a Saccharomyces cerevisiae sec19‐1 mutant strain. The sequenced DNA fragments contain open reading frames of 1338 bp (K.lactis) and 1344 bp (P. pastoris), coding for polypeptides of 445 and 447 residues, respectively. Both sequences fully complement the S. cerevisiae sec19‐1 mutation. They have high degrees of homology with known GDP dissociation inhibitors from yeast species and other eukaryotes. The GenBank Accession Nos of the sequences are AF255332 (K.lactis GDI1) and AY007574 (P. pastoris GDI1).

U2 - 10.1002/yea.736

DO - 10.1002/yea.736

M3 - Article

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