Abstract
The ciel genes of the filamentous fungi Trichoderma reesei and T. harzianum were isolated and characterized. The deduced CREI proteins are 46% identical to the product of the glucose repressor gene creA of Aspergillus nidulans, encoding a DNA-binding protein with zinc fingers of the C2H2 type. The cre1 promoters contain several sequence elements that are identical to the previously identified binding sites for A. nidulans CREA. Steady-state mRNA levels for cre1 of the T. reesei strain QM9414 varied depending on the carbon source, being low on glucose-containing media. These observations suggest that cre1 expression may be autoregulated. The T. reesei strain Rut-C30, a hyperproducer of cellulolytic enzymes, was found to express a truncated form of the cre1 gene (cre1-1) with an ORF corresponding to a protein of 95 amino acids with only one zinc finger. Unlike QM9414 the strain Rut-C30 produced cellulase mRNAs on glucose-containing medium and transformation of the full-length cre1 gene into this strain caused glucose repression of cbh1 expression, demonstrating that cre1 regulates cellulase expression.
| Original language | English |
|---|---|
| Pages (from-to) | 451-460 |
| Number of pages | 10 |
| Journal | Molecular and General Genetics |
| Volume | 251 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 20 Aug 1996 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Filamentous fungus
- Transcriptional regulation
- Zinc fingers
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The glucose repressor gene cre1 of Trichoderma : Isolation and expression of a full length and a truncated mutant form. / Ilmén, Marja; Thrane, Charlotte; Penttilä, Merja.
In: Molecular and General Genetics, Vol. 251, No. 4, 20.08.1996, p. 451-460.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - The glucose repressor gene cre1 of Trichoderma
T2 - Isolation and expression of a full length and a truncated mutant form
AU - Ilmén, Marja
AU - Thrane, Charlotte
AU - Penttilä, Merja
PY - 1996/8/20
Y1 - 1996/8/20
N2 - The ciel genes of the filamentous fungi Trichoderma reesei and T. harzianum were isolated and characterized. The deduced CREI proteins are 46% identical to the product of the glucose repressor gene creA of Aspergillus nidulans, encoding a DNA-binding protein with zinc fingers of the C2H2 type. The cre1 promoters contain several sequence elements that are identical to the previously identified binding sites for A. nidulans CREA. Steady-state mRNA levels for cre1 of the T. reesei strain QM9414 varied depending on the carbon source, being low on glucose-containing media. These observations suggest that cre1 expression may be autoregulated. The T. reesei strain Rut-C30, a hyperproducer of cellulolytic enzymes, was found to express a truncated form of the cre1 gene (cre1-1) with an ORF corresponding to a protein of 95 amino acids with only one zinc finger. Unlike QM9414 the strain Rut-C30 produced cellulase mRNAs on glucose-containing medium and transformation of the full-length cre1 gene into this strain caused glucose repression of cbh1 expression, demonstrating that cre1 regulates cellulase expression.
AB - The ciel genes of the filamentous fungi Trichoderma reesei and T. harzianum were isolated and characterized. The deduced CREI proteins are 46% identical to the product of the glucose repressor gene creA of Aspergillus nidulans, encoding a DNA-binding protein with zinc fingers of the C2H2 type. The cre1 promoters contain several sequence elements that are identical to the previously identified binding sites for A. nidulans CREA. Steady-state mRNA levels for cre1 of the T. reesei strain QM9414 varied depending on the carbon source, being low on glucose-containing media. These observations suggest that cre1 expression may be autoregulated. The T. reesei strain Rut-C30, a hyperproducer of cellulolytic enzymes, was found to express a truncated form of the cre1 gene (cre1-1) with an ORF corresponding to a protein of 95 amino acids with only one zinc finger. Unlike QM9414 the strain Rut-C30 produced cellulase mRNAs on glucose-containing medium and transformation of the full-length cre1 gene into this strain caused glucose repression of cbh1 expression, demonstrating that cre1 regulates cellulase expression.
KW - Filamentous fungus
KW - Transcriptional regulation
KW - Zinc fingers
UR - http://www.scopus.com/inward/record.url?scp=0030036051&partnerID=8YFLogxK
U2 - 10.1007/s004380050189
DO - 10.1007/s004380050189
M3 - Article
C2 - 8709949
AN - SCOPUS:0030036051
VL - 251
SP - 451
EP - 460
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
SN - 1617-4615
IS - 4
ER -