The glucose repressor gene cre1 of Trichoderma

Isolation and expression of a full length and a truncated mutant form

Marja Ilmén, Charlotte Thrane, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

255 Citations (Scopus)

Abstract

The ciel genes of the filamentous fungi Trichoderma reesei and T. harzianum were isolated and characterized. The deduced CREI proteins are 46% identical to the product of the glucose repressor gene creA of Aspergillus nidulans, encoding a DNA-binding protein with zinc fingers of the C2H2 type. The cre1 promoters contain several sequence elements that are identical to the previously identified binding sites for A. nidulans CREA. Steady-state mRNA levels for cre1 of the T. reesei strain QM9414 varied depending on the carbon source, being low on glucose-containing media. These observations suggest that cre1 expression may be autoregulated. The T. reesei strain Rut-C30, a hyperproducer of cellulolytic enzymes, was found to express a truncated form of the cre1 gene (cre1-1) with an ORF corresponding to a protein of 95 amino acids with only one zinc finger. Unlike QM9414 the strain Rut-C30 produced cellulase mRNAs on glucose-containing medium and transformation of the full-length cre1 gene into this strain caused glucose repression of cbh1 expression, demonstrating that cre1 regulates cellulase expression.

Original languageEnglish
Pages (from-to)451-460
Number of pages10
JournalMolecular and General Genetics
Volume251
Issue number4
DOIs
Publication statusPublished - 20 Aug 1996
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma
Glucose
Aspergillus nidulans
Cellulase
Genes
Messenger RNA
Zinc Fingers
DNA-Binding Proteins
Open Reading Frames
Proteins
Fungi
Carbon
Binding Sites
Amino Acids
Enzymes

Keywords

  • Filamentous fungus
  • Transcriptional regulation
  • Zinc fingers

Cite this

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title = "The glucose repressor gene cre1 of Trichoderma: Isolation and expression of a full length and a truncated mutant form",
abstract = "The ciel genes of the filamentous fungi Trichoderma reesei and T. harzianum were isolated and characterized. The deduced CREI proteins are 46{\%} identical to the product of the glucose repressor gene creA of Aspergillus nidulans, encoding a DNA-binding protein with zinc fingers of the C2H2 type. The cre1 promoters contain several sequence elements that are identical to the previously identified binding sites for A. nidulans CREA. Steady-state mRNA levels for cre1 of the T. reesei strain QM9414 varied depending on the carbon source, being low on glucose-containing media. These observations suggest that cre1 expression may be autoregulated. The T. reesei strain Rut-C30, a hyperproducer of cellulolytic enzymes, was found to express a truncated form of the cre1 gene (cre1-1) with an ORF corresponding to a protein of 95 amino acids with only one zinc finger. Unlike QM9414 the strain Rut-C30 produced cellulase mRNAs on glucose-containing medium and transformation of the full-length cre1 gene into this strain caused glucose repression of cbh1 expression, demonstrating that cre1 regulates cellulase expression.",
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The glucose repressor gene cre1 of Trichoderma : Isolation and expression of a full length and a truncated mutant form. / Ilmén, Marja; Thrane, Charlotte; Penttilä, Merja.

In: Molecular and General Genetics, Vol. 251, No. 4, 20.08.1996, p. 451-460.

Research output: Contribution to journalArticleScientificpeer-review

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AB - The ciel genes of the filamentous fungi Trichoderma reesei and T. harzianum were isolated and characterized. The deduced CREI proteins are 46% identical to the product of the glucose repressor gene creA of Aspergillus nidulans, encoding a DNA-binding protein with zinc fingers of the C2H2 type. The cre1 promoters contain several sequence elements that are identical to the previously identified binding sites for A. nidulans CREA. Steady-state mRNA levels for cre1 of the T. reesei strain QM9414 varied depending on the carbon source, being low on glucose-containing media. These observations suggest that cre1 expression may be autoregulated. The T. reesei strain Rut-C30, a hyperproducer of cellulolytic enzymes, was found to express a truncated form of the cre1 gene (cre1-1) with an ORF corresponding to a protein of 95 amino acids with only one zinc finger. Unlike QM9414 the strain Rut-C30 produced cellulase mRNAs on glucose-containing medium and transformation of the full-length cre1 gene into this strain caused glucose repression of cbh1 expression, demonstrating that cre1 regulates cellulase expression.

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