The cis/trans interconversion of the calcium regulating hormone calcitonin is catalyzed by cyclophilin

D. Hubner, Torbjörn Drakenberg, M. Wikström, Sture Forsen, H. Bang, G. Fischer

Research output: Contribution to journalArticleScientificpeer-review

41 Citations (Scopus)

Abstract

The cytosolic peptidyl‐prolyl cis/trans isomerase cyclophilin from pig kidney can accelerate catalytically the cis/trans isomerization of prolyl peptide bonds. One‐ and two‐dimensional 1H NMR spectroscopy was used to prove that the polypeptide hormone calcitonin is a substrate for cyclophilin. Isomerization of only one of the two prolyl peptide bonds is catalyzed significantly. The efficiency of catalysis was calculated by lineshape analysis and NOESY spectroscopy. Cyclosporin A completely blocks the effect of the enzyme on the conformational dynamics of the polypeptide.

Original languageEnglish
Pages (from-to)198 - 203
Number of pages6
JournalFEBS Letters
Volume323
Issue number3
DOIs
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

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Cyclophilins
Calcitonin
Hormones
Isomerization
Calcium
Peptides
cis-trans-Isomerases
Peptidylprolyl Isomerase
Peptide Hormones
Catalysis
Cyclosporine
Nuclear magnetic resonance spectroscopy
Spectrum Analysis
Magnetic Resonance Spectroscopy
Swine
Spectroscopy
Kidney
Substrates
Enzymes

Cite this

Hubner, D., Drakenberg, T., Wikström, M., Forsen, S., Bang, H., & Fischer, G. (1993). The cis/trans interconversion of the calcium regulating hormone calcitonin is catalyzed by cyclophilin. FEBS Letters, 323(3), 198 - 203. https://doi.org/10.1016/0014-5793(93)81338-Z
Hubner, D. ; Drakenberg, Torbjörn ; Wikström, M. ; Forsen, Sture ; Bang, H. ; Fischer, G. / The cis/trans interconversion of the calcium regulating hormone calcitonin is catalyzed by cyclophilin. In: FEBS Letters. 1993 ; Vol. 323, No. 3. pp. 198 - 203.
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Hubner, D, Drakenberg, T, Wikström, M, Forsen, S, Bang, H & Fischer, G 1993, 'The cis/trans interconversion of the calcium regulating hormone calcitonin is catalyzed by cyclophilin', FEBS Letters, vol. 323, no. 3, pp. 198 - 203. https://doi.org/10.1016/0014-5793(93)81338-Z

The cis/trans interconversion of the calcium regulating hormone calcitonin is catalyzed by cyclophilin. / Hubner, D.; Drakenberg, Torbjörn; Wikström, M.; Forsen, Sture; Bang, H.; Fischer, G.

In: FEBS Letters, Vol. 323, No. 3, 1993, p. 198 - 203.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - The cis/trans interconversion of the calcium regulating hormone calcitonin is catalyzed by cyclophilin

AU - Hubner, D.

AU - Drakenberg, Torbjörn

AU - Wikström, M.

AU - Forsen, Sture

AU - Bang, H.

AU - Fischer, G.

PY - 1993

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AB - The cytosolic peptidyl‐prolyl cis/trans isomerase cyclophilin from pig kidney can accelerate catalytically the cis/trans isomerization of prolyl peptide bonds. One‐ and two‐dimensional 1H NMR spectroscopy was used to prove that the polypeptide hormone calcitonin is a substrate for cyclophilin. Isomerization of only one of the two prolyl peptide bonds is catalyzed significantly. The efficiency of catalysis was calculated by lineshape analysis and NOESY spectroscopy. Cyclosporin A completely blocks the effect of the enzyme on the conformational dynamics of the polypeptide.

U2 - 10.1016/0014-5793(93)81338-Z

DO - 10.1016/0014-5793(93)81338-Z

M3 - Article

VL - 323

SP - 198

EP - 203

JO - FEBS Letters

JF - FEBS Letters

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