The in vitro 14C-labelled kraft pulp assay as used for evaluation of laccases and mediators intended for pulp bleaching

Paul Ander; Karin Grabner; Gottfried Kandioller; Ewald Srebotnik; Kristiina Poppius-Levlin; Sylvia Semar; Heidrun Anke; Marja-Leena Niku-Paavola; Liisa Viikari; Kurt Messner

The in vitro ¹⁴C-labelled kraft pulp assay as used for evaluation of laccases and mediators intended for pulp bleaching

Paul Ander, Karin Grabner, Gottfried Kandioller, Ewald Srebotnik, Kristiina Poppius-Levlin, Sylvia Semar, Heidrun Anke, Marja-Leena Niku-Paavola, Liisa Viikari, Kurt Messner

VTT Technical Research Centre of Finland

Swedish University of Agricultural Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific

1 Citation (Scopus)

Abstract

In the presence of K-ferricyanide as oxidant at pH 10, ¹⁴C-ring-labelled synthetic lignin (DHP) was covalently coupled to unbleached kraft pulp. This chemically labelled kraft pulp (CLKP) was used in a small scale (30 mg pulp in 1.5 ml buffer) for testing ligninolytic enzymes and mediators for possible use in pulp bleaching. The results from this assay correlated well with laccase/mediator kraft pulp delignification plus TCF-bleaching. The order of release of label from CLKP was: Laccase>lignin peroxidase>Bj. adusta MnP>Phanerochaete MnP. These results are discussed, as well as the possible relationship to oxidation of 20 mM HBT (1-hydroxybenzotriazole) by the above enzymes.

Original language	English
Title of host publication	7th International Conference on Biotechnology in the Pulp and Paper Industry
Subtitle of host publication	Oral presentations
Place of Publication	Montreal
Publisher	Canadian Pulp and Paper Association (CPPA)
Pages	A111-A115
Volume	A
ISBN (Print)	978-1-896742-34-2
Publication status	Published - 1998
MoE publication type	B3 Non-refereed article in conference proceedings
Event	7th International Conference on Biotechnology in the Pulp and Paper Industry - Vancouver, Canada Duration: 16 Jun 1998 → 19 Jun 1998

Conference

Conference	7th International Conference on Biotechnology in the Pulp and Paper Industry
Country/Territory	Canada
City	Vancouver
Period	16/06/98 → 19/06/98

Cite this

Ander, P., Grabner, K., Kandioller, G., Srebotnik, E., Poppius-Levlin, K., Semar, S., Anke, H., Niku-Paavola, M.-L., Viikari, L., & Messner, K. (1998). The in vitro ¹⁴C-labelled kraft pulp assay as used for evaluation of laccases and mediators intended for pulp bleaching. In 7th International Conference on Biotechnology in the Pulp and Paper Industry: Oral presentations (Vol. A, pp. A111-A115). Canadian Pulp and Paper Association (CPPA).

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title = "The in vitro 14C-labelled kraft pulp assay as used for evaluation of laccases and mediators intended for pulp bleaching",

abstract = "In the presence of K-ferricyanide as oxidant at pH 10, 14C-ring-labelled synthetic lignin (DHP) was covalently coupled to unbleached kraft pulp. This chemically labelled kraft pulp (CLKP) was used in a small scale (30 mg pulp in 1.5 ml buffer) for testing ligninolytic enzymes and mediators for possible use in pulp bleaching. The results from this assay correlated well with laccase/mediator kraft pulp delignification plus TCF-bleaching. The order of release of label from CLKP was: Laccase>lignin peroxidase>Bj. adusta MnP>Phanerochaete MnP. These results are discussed, as well as the possible relationship to oxidation of 20 mM HBT (1-hydroxybenzotriazole) by the above enzymes.",

author = "Paul Ander and Karin Grabner and Gottfried Kandioller and Ewald Srebotnik and Kristiina Poppius-Levlin and Sylvia Semar and Heidrun Anke and Marja-Leena Niku-Paavola and Liisa Viikari and Kurt Messner",

year = "1998",

language = "English",

isbn = "978-1-896742-34-2",

volume = "A",

pages = "A111--A115",

booktitle = "7th International Conference on Biotechnology in the Pulp and Paper Industry",

publisher = "Canadian Pulp and Paper Association (CPPA)",

address = "Canada",

note = "7th International Conference on Biotechnology in the Pulp and Paper Industry ; Conference date: 16-06-1998 Through 19-06-1998",

}

Ander, P, Grabner, K, Kandioller, G, Srebotnik, E, Poppius-Levlin, K, Semar, S, Anke, H, Niku-Paavola, M-L, Viikari, L & Messner, K 1998, The in vitro ¹⁴C-labelled kraft pulp assay as used for evaluation of laccases and mediators intended for pulp bleaching. in 7th International Conference on Biotechnology in the Pulp and Paper Industry: Oral presentations. vol. A, Canadian Pulp and Paper Association (CPPA), Montreal, pp. A111-A115, 7th International Conference on Biotechnology in the Pulp and Paper Industry, Vancouver, British Columbia, Canada, 16/06/98.

The in vitro ¹⁴C-labelled kraft pulp assay as used for evaluation of laccases and mediators intended for pulp bleaching. / Ander, Paul; Grabner, Karin; Kandioller, Gottfried et al.
7th International Conference on Biotechnology in the Pulp and Paper Industry: Oral presentations. Vol. A Montreal: Canadian Pulp and Paper Association (CPPA), 1998. p. A111-A115.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific

TY - GEN

T1 - The in vitro 14C-labelled kraft pulp assay as used for evaluation of laccases and mediators intended for pulp bleaching

AU - Ander, Paul

AU - Grabner, Karin

AU - Kandioller, Gottfried

AU - Srebotnik, Ewald

AU - Poppius-Levlin, Kristiina

AU - Semar, Sylvia

AU - Anke, Heidrun

AU - Niku-Paavola, Marja-Leena

AU - Viikari, Liisa

AU - Messner, Kurt

PY - 1998

Y1 - 1998

N2 - In the presence of K-ferricyanide as oxidant at pH 10, 14C-ring-labelled synthetic lignin (DHP) was covalently coupled to unbleached kraft pulp. This chemically labelled kraft pulp (CLKP) was used in a small scale (30 mg pulp in 1.5 ml buffer) for testing ligninolytic enzymes and mediators for possible use in pulp bleaching. The results from this assay correlated well with laccase/mediator kraft pulp delignification plus TCF-bleaching. The order of release of label from CLKP was: Laccase>lignin peroxidase>Bj. adusta MnP>Phanerochaete MnP. These results are discussed, as well as the possible relationship to oxidation of 20 mM HBT (1-hydroxybenzotriazole) by the above enzymes.

AB - In the presence of K-ferricyanide as oxidant at pH 10, 14C-ring-labelled synthetic lignin (DHP) was covalently coupled to unbleached kraft pulp. This chemically labelled kraft pulp (CLKP) was used in a small scale (30 mg pulp in 1.5 ml buffer) for testing ligninolytic enzymes and mediators for possible use in pulp bleaching. The results from this assay correlated well with laccase/mediator kraft pulp delignification plus TCF-bleaching. The order of release of label from CLKP was: Laccase>lignin peroxidase>Bj. adusta MnP>Phanerochaete MnP. These results are discussed, as well as the possible relationship to oxidation of 20 mM HBT (1-hydroxybenzotriazole) by the above enzymes.

M3 - Conference article in proceedings

SN - 978-1-896742-34-2

VL - A

SP - A111-A115

BT - 7th International Conference on Biotechnology in the Pulp and Paper Industry

PB - Canadian Pulp and Paper Association (CPPA)

CY - Montreal

T2 - 7th International Conference on Biotechnology in the Pulp and Paper Industry

Y2 - 16 June 1998 through 19 June 1998

ER -

Ander P, Grabner K, Kandioller G, Srebotnik E, Poppius-Levlin K, Semar S et al. The in vitro ¹⁴C-labelled kraft pulp assay as used for evaluation of laccases and mediators intended for pulp bleaching. In 7th International Conference on Biotechnology in the Pulp and Paper Industry: Oral presentations. Vol. A. Montreal: Canadian Pulp and Paper Association (CPPA). 1998. p. A111-A115

The in vitro 14C-labelled kraft pulp assay as used for evaluation of laccases and mediators intended for pulp bleaching

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The in vitro ¹⁴C-labelled kraft pulp assay as used for evaluation of laccases and mediators intended for pulp bleaching