The influence of the carrier material on the microbial diversity in packed-bed biofilm reactors

Anu Kapanen, Minna Vikman, Anna Lehtonen, Merja Itävaara

Research output: Contribution to conferenceConference articleScientific

Abstract

Microbial diversity in biofilms on two different types of carrier materials, wood chips (WD) and polyethylene carrier elements (PE) was studied in laboratory-scale packed bed bioreactors treating municipal waste water. Two packed bed bioreactors were set in series, thus enhancing also the removal of compounds more resistant to degradation. With both carrier materials the removal rate of BOD, COD and nutrients was higher in the first reactor. The maximum BOD removal rates in bioreactors R1 (WD) and R2 (PE) were 96% and 95% respectively. PCR-Denaturing Gradient Gel Electrophoresis (DGGE) analysis indicated that the bacterial species dominating the biofilm reactors differed from the diversity in influent waste water. The difference in the first and the second column was particularly obvious with the biofilm on polyethylene carrier material. Contrary to the bacterial diversity in the first bioreactor, there was only one major species detected in the second bioreactor. The bacteria detected belonged to Rhizobium genus. Acidobacteria were present in biofilms on both PE and WD, but were not detected from influent. Biofilm on WD, but not on PE, contained Verrucomicrobia and d-proteobacteria. Furthermore, only one ß-proteobacteria sequence was derived from PE biofilms.
Original languageEnglish
Pages137-138
Publication statusPublished - 2011
MoE publication typeNot Eligible
EventIWA Biofilm Conference 2011 - Shanghai, China
Duration: 27 Oct 201130 Oct 2011

Conference

ConferenceIWA Biofilm Conference 2011
Abbreviated titleIWA Biofilm2011
CountryChina
CityShanghai
Period27/10/1130/10/11

Fingerprint

biofilm
bioreactor
rhizobacterium
reactor
material
electrokinesis
gel
degradation
bacterium
nutrient
removal
rate
waste water

Keywords

  • biofilm
  • bioreactor
  • carrier
  • microbial diversity
  • waste water
  • DGGE

Cite this

Kapanen, A., Vikman, M., Lehtonen, A., & Itävaara, M. (2011). The influence of the carrier material on the microbial diversity in packed-bed biofilm reactors. 137-138. Paper presented at IWA Biofilm Conference 2011, Shanghai, China.
Kapanen, Anu ; Vikman, Minna ; Lehtonen, Anna ; Itävaara, Merja. / The influence of the carrier material on the microbial diversity in packed-bed biofilm reactors. Paper presented at IWA Biofilm Conference 2011, Shanghai, China.
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abstract = "Microbial diversity in biofilms on two different types of carrier materials, wood chips (WD) and polyethylene carrier elements (PE) was studied in laboratory-scale packed bed bioreactors treating municipal waste water. Two packed bed bioreactors were set in series, thus enhancing also the removal of compounds more resistant to degradation. With both carrier materials the removal rate of BOD, COD and nutrients was higher in the first reactor. The maximum BOD removal rates in bioreactors R1 (WD) and R2 (PE) were 96{\%} and 95{\%} respectively. PCR-Denaturing Gradient Gel Electrophoresis (DGGE) analysis indicated that the bacterial species dominating the biofilm reactors differed from the diversity in influent waste water. The difference in the first and the second column was particularly obvious with the biofilm on polyethylene carrier material. Contrary to the bacterial diversity in the first bioreactor, there was only one major species detected in the second bioreactor. The bacteria detected belonged to Rhizobium genus. Acidobacteria were present in biofilms on both PE and WD, but were not detected from influent. Biofilm on WD, but not on PE, contained Verrucomicrobia and d-proteobacteria. Furthermore, only one {\ss}-proteobacteria sequence was derived from PE biofilms.",
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Kapanen, A, Vikman, M, Lehtonen, A & Itävaara, M 2011, 'The influence of the carrier material on the microbial diversity in packed-bed biofilm reactors' Paper presented at IWA Biofilm Conference 2011, Shanghai, China, 27/10/11 - 30/10/11, pp. 137-138.

The influence of the carrier material on the microbial diversity in packed-bed biofilm reactors. / Kapanen, Anu; Vikman, Minna; Lehtonen, Anna; Itävaara, Merja.

2011. 137-138 Paper presented at IWA Biofilm Conference 2011, Shanghai, China.

Research output: Contribution to conferenceConference articleScientific

TY - CONF

T1 - The influence of the carrier material on the microbial diversity in packed-bed biofilm reactors

AU - Kapanen, Anu

AU - Vikman, Minna

AU - Lehtonen, Anna

AU - Itävaara, Merja

N1 - Project code: 38049

PY - 2011

Y1 - 2011

N2 - Microbial diversity in biofilms on two different types of carrier materials, wood chips (WD) and polyethylene carrier elements (PE) was studied in laboratory-scale packed bed bioreactors treating municipal waste water. Two packed bed bioreactors were set in series, thus enhancing also the removal of compounds more resistant to degradation. With both carrier materials the removal rate of BOD, COD and nutrients was higher in the first reactor. The maximum BOD removal rates in bioreactors R1 (WD) and R2 (PE) were 96% and 95% respectively. PCR-Denaturing Gradient Gel Electrophoresis (DGGE) analysis indicated that the bacterial species dominating the biofilm reactors differed from the diversity in influent waste water. The difference in the first and the second column was particularly obvious with the biofilm on polyethylene carrier material. Contrary to the bacterial diversity in the first bioreactor, there was only one major species detected in the second bioreactor. The bacteria detected belonged to Rhizobium genus. Acidobacteria were present in biofilms on both PE and WD, but were not detected from influent. Biofilm on WD, but not on PE, contained Verrucomicrobia and d-proteobacteria. Furthermore, only one ß-proteobacteria sequence was derived from PE biofilms.

AB - Microbial diversity in biofilms on two different types of carrier materials, wood chips (WD) and polyethylene carrier elements (PE) was studied in laboratory-scale packed bed bioreactors treating municipal waste water. Two packed bed bioreactors were set in series, thus enhancing also the removal of compounds more resistant to degradation. With both carrier materials the removal rate of BOD, COD and nutrients was higher in the first reactor. The maximum BOD removal rates in bioreactors R1 (WD) and R2 (PE) were 96% and 95% respectively. PCR-Denaturing Gradient Gel Electrophoresis (DGGE) analysis indicated that the bacterial species dominating the biofilm reactors differed from the diversity in influent waste water. The difference in the first and the second column was particularly obvious with the biofilm on polyethylene carrier material. Contrary to the bacterial diversity in the first bioreactor, there was only one major species detected in the second bioreactor. The bacteria detected belonged to Rhizobium genus. Acidobacteria were present in biofilms on both PE and WD, but were not detected from influent. Biofilm on WD, but not on PE, contained Verrucomicrobia and d-proteobacteria. Furthermore, only one ß-proteobacteria sequence was derived from PE biofilms.

KW - biofilm

KW - bioreactor

KW - carrier

KW - microbial diversity

KW - waste water

KW - DGGE

M3 - Conference article

SP - 137

EP - 138

ER -

Kapanen A, Vikman M, Lehtonen A, Itävaara M. The influence of the carrier material on the microbial diversity in packed-bed biofilm reactors. 2011. Paper presented at IWA Biofilm Conference 2011, Shanghai, China.