The introduction of the fungal D-galacturonate pathway enables the consumption of D-galacturonic acid by Saccharomyces cerevisiae

Alessandra Biz (Corresponding Author), Maura Harumi Sugai-Guérios, Joosu Kuivanen, Hannu Maaheimo, Nadja Krieger, David Alexander Mitchell, Peter Richard (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)

Abstract

Background: Pectin-rich wastes, such as citrus pulp and sugar beet pulp, are produced in considerable amounts by the juice and sugar industry and could be used as raw materials for biorefineries. One possible process in such biorefineries is the hydrolysis of these wastes and the subsequent production of ethanol. However, the ethanol-producing organism of choice, Saccharomyces cerevisiae, is not able to catabolize d-galacturonic acid, which represents a considerable amount of the sugars in the hydrolysate, namely, 18 % (w/w) from citrus pulp and 16 % (w/w) sugar beet pulp. Results: In the current work, we describe the construction of a strain of S. cerevisiae in which the five genes of the fungal reductive pathway for d-galacturonic acid catabolism were integrated into the yeast chromosomes: gaaA, gaaC and gaaD from Aspergillus niger and lgd1 from Trichoderma reesei, and the recently described d-galacturonic acid transporter protein, gat1, from Neurospora crassa. This strain metabolized d-galacturonic acid in a medium containing d-fructose as co-substrate. Conclusion: This work is the first demonstration of the expression of a functional heterologous pathway for d-galacturonic acid catabolism in Saccharomyces cerevisiae. It is a preliminary step for engineering a yeast strain for the fermentation of pectin-rich substrates to ethanol.
Original languageEnglish
JournalMicrobial Cell Factories
Volume15
Issue number144
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

Yeast
Saccharomyces cerevisiae
Pulp
Acids
Sugar beets
Beta vulgaris
Ethanol
Citrus
Fungal Genes
Yeasts
Sugar industry
Neurospora crassa
Fructose
Trichoderma
Aspergillus niger
Aspergillus
Substrates
Chromosomes
Sugars
Fermentation

Keywords

  • ethanol
  • d-galacturonic acid
  • Saccharomyces cerevisiae
  • citrus pulp
  • metabolic engineering

Cite this

Biz, Alessandra ; Sugai-Guérios, Maura Harumi ; Kuivanen, Joosu ; Maaheimo, Hannu ; Krieger, Nadja ; Mitchell, David Alexander ; Richard, Peter. / The introduction of the fungal D-galacturonate pathway enables the consumption of D-galacturonic acid by Saccharomyces cerevisiae. In: Microbial Cell Factories. 2016 ; Vol. 15, No. 144.
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abstract = "Background: Pectin-rich wastes, such as citrus pulp and sugar beet pulp, are produced in considerable amounts by the juice and sugar industry and could be used as raw materials for biorefineries. One possible process in such biorefineries is the hydrolysis of these wastes and the subsequent production of ethanol. However, the ethanol-producing organism of choice, Saccharomyces cerevisiae, is not able to catabolize d-galacturonic acid, which represents a considerable amount of the sugars in the hydrolysate, namely, 18 {\%} (w/w) from citrus pulp and 16 {\%} (w/w) sugar beet pulp. Results: In the current work, we describe the construction of a strain of S. cerevisiae in which the five genes of the fungal reductive pathway for d-galacturonic acid catabolism were integrated into the yeast chromosomes: gaaA, gaaC and gaaD from Aspergillus niger and lgd1 from Trichoderma reesei, and the recently described d-galacturonic acid transporter protein, gat1, from Neurospora crassa. This strain metabolized d-galacturonic acid in a medium containing d-fructose as co-substrate. Conclusion: This work is the first demonstration of the expression of a functional heterologous pathway for d-galacturonic acid catabolism in Saccharomyces cerevisiae. It is a preliminary step for engineering a yeast strain for the fermentation of pectin-rich substrates to ethanol.",
keywords = "ethanol, d-galacturonic acid, Saccharomyces cerevisiae, citrus pulp, metabolic engineering",
author = "Alessandra Biz and Sugai-Gu{\'e}rios, {Maura Harumi} and Joosu Kuivanen and Hannu Maaheimo and Nadja Krieger and Mitchell, {David Alexander} and Peter Richard",
note = "SDA: SHP: Bioeconomy Project code: 100686",
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The introduction of the fungal D-galacturonate pathway enables the consumption of D-galacturonic acid by Saccharomyces cerevisiae. / Biz, Alessandra (Corresponding Author); Sugai-Guérios, Maura Harumi; Kuivanen, Joosu; Maaheimo, Hannu; Krieger, Nadja; Mitchell, David Alexander; Richard, Peter (Corresponding Author).

In: Microbial Cell Factories, Vol. 15, No. 144, 2016.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The introduction of the fungal D-galacturonate pathway enables the consumption of D-galacturonic acid by Saccharomyces cerevisiae

AU - Biz, Alessandra

AU - Sugai-Guérios, Maura Harumi

AU - Kuivanen, Joosu

AU - Maaheimo, Hannu

AU - Krieger, Nadja

AU - Mitchell, David Alexander

AU - Richard, Peter

N1 - SDA: SHP: Bioeconomy Project code: 100686

PY - 2016

Y1 - 2016

N2 - Background: Pectin-rich wastes, such as citrus pulp and sugar beet pulp, are produced in considerable amounts by the juice and sugar industry and could be used as raw materials for biorefineries. One possible process in such biorefineries is the hydrolysis of these wastes and the subsequent production of ethanol. However, the ethanol-producing organism of choice, Saccharomyces cerevisiae, is not able to catabolize d-galacturonic acid, which represents a considerable amount of the sugars in the hydrolysate, namely, 18 % (w/w) from citrus pulp and 16 % (w/w) sugar beet pulp. Results: In the current work, we describe the construction of a strain of S. cerevisiae in which the five genes of the fungal reductive pathway for d-galacturonic acid catabolism were integrated into the yeast chromosomes: gaaA, gaaC and gaaD from Aspergillus niger and lgd1 from Trichoderma reesei, and the recently described d-galacturonic acid transporter protein, gat1, from Neurospora crassa. This strain metabolized d-galacturonic acid in a medium containing d-fructose as co-substrate. Conclusion: This work is the first demonstration of the expression of a functional heterologous pathway for d-galacturonic acid catabolism in Saccharomyces cerevisiae. It is a preliminary step for engineering a yeast strain for the fermentation of pectin-rich substrates to ethanol.

AB - Background: Pectin-rich wastes, such as citrus pulp and sugar beet pulp, are produced in considerable amounts by the juice and sugar industry and could be used as raw materials for biorefineries. One possible process in such biorefineries is the hydrolysis of these wastes and the subsequent production of ethanol. However, the ethanol-producing organism of choice, Saccharomyces cerevisiae, is not able to catabolize d-galacturonic acid, which represents a considerable amount of the sugars in the hydrolysate, namely, 18 % (w/w) from citrus pulp and 16 % (w/w) sugar beet pulp. Results: In the current work, we describe the construction of a strain of S. cerevisiae in which the five genes of the fungal reductive pathway for d-galacturonic acid catabolism were integrated into the yeast chromosomes: gaaA, gaaC and gaaD from Aspergillus niger and lgd1 from Trichoderma reesei, and the recently described d-galacturonic acid transporter protein, gat1, from Neurospora crassa. This strain metabolized d-galacturonic acid in a medium containing d-fructose as co-substrate. Conclusion: This work is the first demonstration of the expression of a functional heterologous pathway for d-galacturonic acid catabolism in Saccharomyces cerevisiae. It is a preliminary step for engineering a yeast strain for the fermentation of pectin-rich substrates to ethanol.

KW - ethanol

KW - d-galacturonic acid

KW - Saccharomyces cerevisiae

KW - citrus pulp

KW - metabolic engineering

U2 - 10.1186/s12934-016-0544-1

DO - 10.1186/s12934-016-0544-1

M3 - Article

VL - 15

JO - Microbial Cell Factories

JF - Microbial Cell Factories

SN - 1475-2859

IS - 144

ER -