Abstract
| Original language | English |
|---|---|
| Journal | Microbial Cell Factories |
| Volume | 15 |
| Issue number | 144 |
| DOIs | |
| Publication status | Published - 2016 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- ethanol
- d-galacturonic acid
- Saccharomyces cerevisiae
- citrus pulp
- metabolic engineering
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The introduction of the fungal D-galacturonate pathway enables the consumption of D-galacturonic acid by Saccharomyces cerevisiae. / Biz, Alessandra (Corresponding Author); Sugai-Guérios, Maura Harumi; Kuivanen, Joosu; Maaheimo, Hannu; Krieger, Nadja; Mitchell, David Alexander; Richard, Peter (Corresponding Author).
In: Microbial Cell Factories, Vol. 15, No. 144, 2016.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - The introduction of the fungal D-galacturonate pathway enables the consumption of D-galacturonic acid by Saccharomyces cerevisiae
AU - Biz, Alessandra
AU - Sugai-Guérios, Maura Harumi
AU - Kuivanen, Joosu
AU - Maaheimo, Hannu
AU - Krieger, Nadja
AU - Mitchell, David Alexander
AU - Richard, Peter
N1 - SDA: SHP: Bioeconomy Project code: 100686
PY - 2016
Y1 - 2016
N2 - Background: Pectin-rich wastes, such as citrus pulp and sugar beet pulp, are produced in considerable amounts by the juice and sugar industry and could be used as raw materials for biorefineries. One possible process in such biorefineries is the hydrolysis of these wastes and the subsequent production of ethanol. However, the ethanol-producing organism of choice, Saccharomyces cerevisiae, is not able to catabolize d-galacturonic acid, which represents a considerable amount of the sugars in the hydrolysate, namely, 18 % (w/w) from citrus pulp and 16 % (w/w) sugar beet pulp. Results: In the current work, we describe the construction of a strain of S. cerevisiae in which the five genes of the fungal reductive pathway for d-galacturonic acid catabolism were integrated into the yeast chromosomes: gaaA, gaaC and gaaD from Aspergillus niger and lgd1 from Trichoderma reesei, and the recently described d-galacturonic acid transporter protein, gat1, from Neurospora crassa. This strain metabolized d-galacturonic acid in a medium containing d-fructose as co-substrate. Conclusion: This work is the first demonstration of the expression of a functional heterologous pathway for d-galacturonic acid catabolism in Saccharomyces cerevisiae. It is a preliminary step for engineering a yeast strain for the fermentation of pectin-rich substrates to ethanol.
AB - Background: Pectin-rich wastes, such as citrus pulp and sugar beet pulp, are produced in considerable amounts by the juice and sugar industry and could be used as raw materials for biorefineries. One possible process in such biorefineries is the hydrolysis of these wastes and the subsequent production of ethanol. However, the ethanol-producing organism of choice, Saccharomyces cerevisiae, is not able to catabolize d-galacturonic acid, which represents a considerable amount of the sugars in the hydrolysate, namely, 18 % (w/w) from citrus pulp and 16 % (w/w) sugar beet pulp. Results: In the current work, we describe the construction of a strain of S. cerevisiae in which the five genes of the fungal reductive pathway for d-galacturonic acid catabolism were integrated into the yeast chromosomes: gaaA, gaaC and gaaD from Aspergillus niger and lgd1 from Trichoderma reesei, and the recently described d-galacturonic acid transporter protein, gat1, from Neurospora crassa. This strain metabolized d-galacturonic acid in a medium containing d-fructose as co-substrate. Conclusion: This work is the first demonstration of the expression of a functional heterologous pathway for d-galacturonic acid catabolism in Saccharomyces cerevisiae. It is a preliminary step for engineering a yeast strain for the fermentation of pectin-rich substrates to ethanol.
KW - ethanol
KW - d-galacturonic acid
KW - Saccharomyces cerevisiae
KW - citrus pulp
KW - metabolic engineering
U2 - 10.1186/s12934-016-0544-1
DO - 10.1186/s12934-016-0544-1
M3 - Article
VL - 15
JO - Microbial Cell Factories
JF - Microbial Cell Factories
SN - 1475-2859
IS - 144
ER -