The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides

Pia Kontula; Maija-Liisa Suihko; Tapani Suortti; Maija Tenkanen; Tiina Mattila-Sandholm; Atte von Wright

doi:10.1006/fmic.1999.0268

The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides

Pia Kontula (Corresponding Author), Maija-Liisa Suihko, Tapani Suortti, Maija Tenkanen, Tiina Mattila-Sandholm, Atte von Wright

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

28 Citations (Scopus)

Abstract

Lactic acid bacteria (LAB) were isolated from human colon biopsies on LAMVAB by enrichment with different substrates such as lactose derivatives, rye arabinoxylo-oligosaccharides and rye fractions. The selected isolates were tested for their ability to adhere to Caco-2 cells. Only Lactobacillus species were enriched under these conditions. From 161 isolates screened, 28% were identified by ribotyping as Lactobacillus rhamnosus, 29% as L. salivarius, 14% as L. cellobiosus, 13% as L. paracasei and the rest remained unidentified.L. rhamnosus was preferentially enriched by lactulose, L. salivarius by lactobionic acid, L. cellobiosus by lactitol and L. paracasei by arabinoxylo-oligosaccharides. The biopsy-derived strains L. rhamnosus E-97948 andL. paracasei E-97949 have potential for further evaluations in their probiotic and technological properties. Lactulose may have prebiotic effects on colonic LAB by favouring their growth.

Original language	English
Pages (from-to)	13-22
Journal	Food Microbiology
Volume	17
Issue number	1
DOIs	https://doi.org/10.1006/fmic.1999.0268
Publication status	Published - 2000
MoE publication type	A1 Journal article-refereed

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title = "The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides",

abstract = "Lactic acid bacteria (LAB) were isolated from human colon biopsies on LAMVAB by enrichment with different substrates such as lactose derivatives, rye arabinoxylo-oligosaccharides and rye fractions. The selected isolates were tested for their ability to adhere to Caco-2 cells. Only Lactobacillus species were enriched under these conditions. From 161 isolates screened, 28% were identified by ribotyping as Lactobacillus rhamnosus, 29% as L. salivarius, 14% as L. cellobiosus, 13% as L. paracasei and the rest remained unidentified.L. rhamnosus was preferentially enriched by lactulose, L. salivarius by lactobionic acid, L. cellobiosus by lactitol and L. paracasei by arabinoxylo-oligosaccharides. The biopsy-derived strains L. rhamnosus E-97948 andL. paracasei E-97949 have potential for further evaluations in their probiotic and technological properties. Lactulose may have prebiotic effects on colonic LAB by favouring their growth.",

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T1 - The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides

AU - Kontula, Pia

AU - Suihko, Maija-Liisa

AU - Suortti, Tapani

AU - Tenkanen, Maija

AU - Mattila-Sandholm, Tiina

AU - von Wright, Atte

PY - 2000

Y1 - 2000

N2 - Lactic acid bacteria (LAB) were isolated from human colon biopsies on LAMVAB by enrichment with different substrates such as lactose derivatives, rye arabinoxylo-oligosaccharides and rye fractions. The selected isolates were tested for their ability to adhere to Caco-2 cells. Only Lactobacillus species were enriched under these conditions. From 161 isolates screened, 28% were identified by ribotyping as Lactobacillus rhamnosus, 29% as L. salivarius, 14% as L. cellobiosus, 13% as L. paracasei and the rest remained unidentified.L. rhamnosus was preferentially enriched by lactulose, L. salivarius by lactobionic acid, L. cellobiosus by lactitol and L. paracasei by arabinoxylo-oligosaccharides. The biopsy-derived strains L. rhamnosus E-97948 andL. paracasei E-97949 have potential for further evaluations in their probiotic and technological properties. Lactulose may have prebiotic effects on colonic LAB by favouring their growth.

AB - Lactic acid bacteria (LAB) were isolated from human colon biopsies on LAMVAB by enrichment with different substrates such as lactose derivatives, rye arabinoxylo-oligosaccharides and rye fractions. The selected isolates were tested for their ability to adhere to Caco-2 cells. Only Lactobacillus species were enriched under these conditions. From 161 isolates screened, 28% were identified by ribotyping as Lactobacillus rhamnosus, 29% as L. salivarius, 14% as L. cellobiosus, 13% as L. paracasei and the rest remained unidentified.L. rhamnosus was preferentially enriched by lactulose, L. salivarius by lactobionic acid, L. cellobiosus by lactitol and L. paracasei by arabinoxylo-oligosaccharides. The biopsy-derived strains L. rhamnosus E-97948 andL. paracasei E-97949 have potential for further evaluations in their probiotic and technological properties. Lactulose may have prebiotic effects on colonic LAB by favouring their growth.

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JO - Food Microbiology

JF - Food Microbiology

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ER -

The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides

Abstract

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