The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides

Pia Kontula (Corresponding Author), Maija-Liisa Suihko, Tapani Suortti, Maija Tenkanen, Tiina Mattila-Sandholm, Atte von Wright

Research output: Contribution to journalArticleScientificpeer-review

23 Citations (Scopus)

Abstract

Lactic acid bacteria (LAB) were isolated from human colon biopsies on LAMVAB by enrichment with different substrates such as lactose derivatives, rye arabinoxylo-oligosaccharides and rye fractions. The selected isolates were tested for their ability to adhere to Caco-2 cells. Only Lactobacillus species were enriched under these conditions. From 161 isolates screened, 28% were identified by ribotyping as Lactobacillus rhamnosus, 29% as L. salivarius, 14% as L. cellobiosus, 13% as L. paracasei and the rest remained unidentified.L. rhamnosus was preferentially enriched by lactulose, L. salivarius by lactobionic acid, L. cellobiosus by lactitol and L. paracasei by arabinoxylo-oligosaccharides. The biopsy-derived strains L. rhamnosus E-97948 andL. paracasei E-97949 have potential for further evaluations in their probiotic and technological properties. Lactulose may have prebiotic effects on colonic LAB by favouring their growth.

Original languageEnglish
Pages (from-to)13 - 22
Number of pages10
JournalFood Microbiology
Volume17
Issue number1
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

Fingerprint

Lactobacillus rhamnosus
Lactose
Oligosaccharides
oligosaccharides
rye
lactose
lactic acid bacteria
Lactulose
biopsy
lactulose
Lactic Acid
chemical derivatives
Bacteria
Biopsy
lactitol
Lactobacillus salivarius
Ribotyping
Lactobacillus paracasei
Lactobacillus fermentum
Prebiotics

Cite this

Kontula, Pia ; Suihko, Maija-Liisa ; Suortti, Tapani ; Tenkanen, Maija ; Mattila-Sandholm, Tiina ; von Wright, Atte. / The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides. In: Food Microbiology. 2000 ; Vol. 17, No. 1. pp. 13 - 22.
@article{608c20758783437c923badbbd21c8dbb,
title = "The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides",
abstract = "Lactic acid bacteria (LAB) were isolated from human colon biopsies on LAMVAB by enrichment with different substrates such as lactose derivatives, rye arabinoxylo-oligosaccharides and rye fractions. The selected isolates were tested for their ability to adhere to Caco-2 cells. Only Lactobacillus species were enriched under these conditions. From 161 isolates screened, 28{\%} were identified by ribotyping as Lactobacillus rhamnosus, 29{\%} as L. salivarius, 14{\%} as L. cellobiosus, 13{\%} as L. paracasei and the rest remained unidentified.L. rhamnosus was preferentially enriched by lactulose, L. salivarius by lactobionic acid, L. cellobiosus by lactitol and L. paracasei by arabinoxylo-oligosaccharides. The biopsy-derived strains L. rhamnosus E-97948 andL. paracasei E-97949 have potential for further evaluations in their probiotic and technological properties. Lactulose may have prebiotic effects on colonic LAB by favouring their growth.",
author = "Pia Kontula and Maija-Liisa Suihko and Tapani Suortti and Maija Tenkanen and Tiina Mattila-Sandholm and {von Wright}, Atte",
year = "2000",
doi = "10.1006/fmic.1999.0268",
language = "English",
volume = "17",
pages = "13 -- 22",
journal = "Food Microbiology",
issn = "0740-0020",
publisher = "Elsevier",
number = "1",

}

The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides. / Kontula, Pia (Corresponding Author); Suihko, Maija-Liisa; Suortti, Tapani; Tenkanen, Maija; Mattila-Sandholm, Tiina; von Wright, Atte.

In: Food Microbiology, Vol. 17, No. 1, 2000, p. 13 - 22.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides

AU - Kontula, Pia

AU - Suihko, Maija-Liisa

AU - Suortti, Tapani

AU - Tenkanen, Maija

AU - Mattila-Sandholm, Tiina

AU - von Wright, Atte

PY - 2000

Y1 - 2000

N2 - Lactic acid bacteria (LAB) were isolated from human colon biopsies on LAMVAB by enrichment with different substrates such as lactose derivatives, rye arabinoxylo-oligosaccharides and rye fractions. The selected isolates were tested for their ability to adhere to Caco-2 cells. Only Lactobacillus species were enriched under these conditions. From 161 isolates screened, 28% were identified by ribotyping as Lactobacillus rhamnosus, 29% as L. salivarius, 14% as L. cellobiosus, 13% as L. paracasei and the rest remained unidentified.L. rhamnosus was preferentially enriched by lactulose, L. salivarius by lactobionic acid, L. cellobiosus by lactitol and L. paracasei by arabinoxylo-oligosaccharides. The biopsy-derived strains L. rhamnosus E-97948 andL. paracasei E-97949 have potential for further evaluations in their probiotic and technological properties. Lactulose may have prebiotic effects on colonic LAB by favouring their growth.

AB - Lactic acid bacteria (LAB) were isolated from human colon biopsies on LAMVAB by enrichment with different substrates such as lactose derivatives, rye arabinoxylo-oligosaccharides and rye fractions. The selected isolates were tested for their ability to adhere to Caco-2 cells. Only Lactobacillus species were enriched under these conditions. From 161 isolates screened, 28% were identified by ribotyping as Lactobacillus rhamnosus, 29% as L. salivarius, 14% as L. cellobiosus, 13% as L. paracasei and the rest remained unidentified.L. rhamnosus was preferentially enriched by lactulose, L. salivarius by lactobionic acid, L. cellobiosus by lactitol and L. paracasei by arabinoxylo-oligosaccharides. The biopsy-derived strains L. rhamnosus E-97948 andL. paracasei E-97949 have potential for further evaluations in their probiotic and technological properties. Lactulose may have prebiotic effects on colonic LAB by favouring their growth.

U2 - 10.1006/fmic.1999.0268

DO - 10.1006/fmic.1999.0268

M3 - Article

VL - 17

SP - 13

EP - 22

JO - Food Microbiology

JF - Food Microbiology

SN - 0740-0020

IS - 1

ER -