The lager yeast Saccharomyces pastorianus removes and transforms Fusarium trichothecene mycotoxins during fermentation of brewer's wort

Alexis V. Nathanail, Brian Gibson, Li Han, Kimmo Peltonen, Velimatti Ollilainen, Marika Jestoi, Arja Laitila

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

An investigation was conducted to determine the fate of deoxynivalenol, deoxynivalenol-3-glucoside, HT-2 toxin and T-2 toxin, during a four-day fermentation with the lager yeast Saccharomyces pastorianus. The influence of excessive mycotoxin concentrations on yeast growth, productivity and viability were also assessed. Mycotoxins were dosed at varying concentrations to 11.5 Plato wort. Analysis of yeast revealed that presence of the toxins even at concentrations up to 10,000 lg/L had little or no effect on sugar utilisation, alcohol production, pH, yeast growth or cell viability. Of the dosed toxin amounts 9-34% were removed by the end of fermentation, due to physical binding and/or biotransformation by yeast. Deoxynivalenol-3-glucoside was not reverted to its toxic precursor during fermentation. Processing of full-scan liquid chromatography-quadrupole time-of-flight-mass spectrometry (LC-QTOF-MS) data with MetaboLynxTM and subsequent LC-QTOF-MS/MS measurements resulted in annotation of several putative metabolites. De(acetylation), glucosylation and sulfonation were the main metabolic pathways activated.
Original languageEnglish
Pages (from-to)448-455
JournalFood Chemistry
Volume203
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichothecenes
brewers yeast
Beer
Saccharomyces
trichothecenes
Mycotoxins
Fusarium
Yeast
Fermentation
mycotoxins
deoxynivalenol-3-glucoside
Yeasts
fermentation
yeasts
liquid chromatography
Liquid chromatography
toxins
Liquid Chromatography
mass spectrometry
Mass spectrometry

Keywords

  • beer
  • biotransformation
  • liquid chromatography-high resolution mass spectrometry
  • metabolic fate
  • modified mycotoxins

Cite this

Nathanail, Alexis V. ; Gibson, Brian ; Han, Li ; Peltonen, Kimmo ; Ollilainen, Velimatti ; Jestoi, Marika ; Laitila, Arja. / The lager yeast Saccharomyces pastorianus removes and transforms Fusarium trichothecene mycotoxins during fermentation of brewer's wort. In: Food Chemistry. 2016 ; Vol. 203. pp. 448-455.
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abstract = "An investigation was conducted to determine the fate of deoxynivalenol, deoxynivalenol-3-glucoside, HT-2 toxin and T-2 toxin, during a four-day fermentation with the lager yeast Saccharomyces pastorianus. The influence of excessive mycotoxin concentrations on yeast growth, productivity and viability were also assessed. Mycotoxins were dosed at varying concentrations to 11.5 Plato wort. Analysis of yeast revealed that presence of the toxins even at concentrations up to 10,000 lg/L had little or no effect on sugar utilisation, alcohol production, pH, yeast growth or cell viability. Of the dosed toxin amounts 9-34{\%} were removed by the end of fermentation, due to physical binding and/or biotransformation by yeast. Deoxynivalenol-3-glucoside was not reverted to its toxic precursor during fermentation. Processing of full-scan liquid chromatography-quadrupole time-of-flight-mass spectrometry (LC-QTOF-MS) data with MetaboLynxTM and subsequent LC-QTOF-MS/MS measurements resulted in annotation of several putative metabolites. De(acetylation), glucosylation and sulfonation were the main metabolic pathways activated.",
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The lager yeast Saccharomyces pastorianus removes and transforms Fusarium trichothecene mycotoxins during fermentation of brewer's wort. / Nathanail, Alexis V.; Gibson, Brian; Han, Li; Peltonen, Kimmo; Ollilainen, Velimatti; Jestoi, Marika; Laitila, Arja.

In: Food Chemistry, Vol. 203, 2016, p. 448-455.

Research output: Contribution to journalArticleScientificpeer-review

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