Abstract
An investigation was conducted to determine the fate of
deoxynivalenol, deoxynivalenol-3-glucoside, HT-2 toxin
and T-2 toxin, during a four-day fermentation with the
lager yeast Saccharomyces pastorianus. The influence of
excessive mycotoxin concentrations on yeast growth,
productivity and viability were also assessed. Mycotoxins
were dosed at varying concentrations to 11.5 Plato wort.
Analysis of yeast revealed that presence of the toxins
even at concentrations up to 10,000 lg/L had little or no
effect on sugar utilisation, alcohol production, pH,
yeast growth or cell viability. Of the dosed toxin
amounts 9-34% were removed by the end of fermentation,
due to physical binding and/or biotransformation by
yeast. Deoxynivalenol-3-glucoside was not reverted to its
toxic precursor during fermentation. Processing of
full-scan liquid chromatography-quadrupole
time-of-flight-mass spectrometry (LC-QTOF-MS) data with
MetaboLynxTM and subsequent LC-QTOF-MS/MS measurements
resulted in annotation of several putative metabolites.
De(acetylation), glucosylation and sulfonation were the
main metabolic pathways activated.
| Original language | English |
|---|---|
| Pages (from-to) | 448-455 |
| Journal | Food Chemistry |
| Volume | 203 |
| DOIs | |
| Publication status | Published - 2016 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- beer
- biotransformation
- liquid chromatography-high resolution mass spectrometry
- metabolic fate
- modified mycotoxins