The microbial impact on the sorption behaviour of selenite in an acidic, nutrient-poor boreal bog

M. Lusa (Corresponding Author), M. Bomberg, H. Aromaa, J. Knuutinen, J. Lehto

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

79Se is among the most important long lived radionuclides in spent nuclear fuel and selenite, SeO32-, is its typical form in intermediate redox potential. The sorption behaviour of selenite and the bacterial impact on the selenite sorption in a 7-m-deep profile of a nutrient-poor boreal bog was studied using batch sorption experiments. The batch distribution coefficient (Kd) values of selenite decreased as a function of sampling depth and highest Kd values, 6600 L/kg dry weight (DW), were observed in the surface moss and the lowest in the bottom clay at 1700 L/kg DW. The overall maximum sorption was observed at pH between 3 and 4 and the Kd values were significantly higher in unsterilized compared to sterilized samples. The removal of selenite from solution by Pseudomonas sp., Burkholderia sp., Rhodococcus sp. and Paenibacillus sp. strains isolated from the bog was affected by incubation temperature and time. In addition, the incubation of sterilized surface moss, subsurface peat and gyttja samples with added bacteria effectively removed selenite from the solution and on average 65% of selenite was removed when Pseudomonas sp. or Burkholderia sp. strains were used. Our results demonstrate the important role of bacteria for the removal of selenite from the solution phase in the bog environment, having a high organic matter content and a low pH.
Original languageEnglish
Pages (from-to)85-96
JournalJournal of Environmental Radioactivity
Volume147
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

Selenious Acid
selenite
Wetlands
bog
Nutrients
Sorption
sorption
Food
nutrient
Bacteria
Burkholderia
Peat
Spent fuels
Nuclear fuels
Pseudomonas
Radioisotopes
moss
Biological materials
Clay
Sphagnopsida

Keywords

  • sorption
  • biosorption
  • selenite
  • peat
  • bacteria
  • nuclear waste

Cite this

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title = "The microbial impact on the sorption behaviour of selenite in an acidic, nutrient-poor boreal bog",
abstract = "79Se is among the most important long lived radionuclides in spent nuclear fuel and selenite, SeO32-, is its typical form in intermediate redox potential. The sorption behaviour of selenite and the bacterial impact on the selenite sorption in a 7-m-deep profile of a nutrient-poor boreal bog was studied using batch sorption experiments. The batch distribution coefficient (Kd) values of selenite decreased as a function of sampling depth and highest Kd values, 6600 L/kg dry weight (DW), were observed in the surface moss and the lowest in the bottom clay at 1700 L/kg DW. The overall maximum sorption was observed at pH between 3 and 4 and the Kd values were significantly higher in unsterilized compared to sterilized samples. The removal of selenite from solution by Pseudomonas sp., Burkholderia sp., Rhodococcus sp. and Paenibacillus sp. strains isolated from the bog was affected by incubation temperature and time. In addition, the incubation of sterilized surface moss, subsurface peat and gyttja samples with added bacteria effectively removed selenite from the solution and on average 65{\%} of selenite was removed when Pseudomonas sp. or Burkholderia sp. strains were used. Our results demonstrate the important role of bacteria for the removal of selenite from the solution phase in the bog environment, having a high organic matter content and a low pH.",
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The microbial impact on the sorption behaviour of selenite in an acidic, nutrient-poor boreal bog. / Lusa, M. (Corresponding Author); Bomberg, M.; Aromaa, H.; Knuutinen, J.; Lehto, J.

In: Journal of Environmental Radioactivity, Vol. 147, 2015, p. 85-96.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The microbial impact on the sorption behaviour of selenite in an acidic, nutrient-poor boreal bog

AU - Lusa, M.

AU - Bomberg, M.

AU - Aromaa, H.

AU - Knuutinen, J.

AU - Lehto, J.

PY - 2015

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N2 - 79Se is among the most important long lived radionuclides in spent nuclear fuel and selenite, SeO32-, is its typical form in intermediate redox potential. The sorption behaviour of selenite and the bacterial impact on the selenite sorption in a 7-m-deep profile of a nutrient-poor boreal bog was studied using batch sorption experiments. The batch distribution coefficient (Kd) values of selenite decreased as a function of sampling depth and highest Kd values, 6600 L/kg dry weight (DW), were observed in the surface moss and the lowest in the bottom clay at 1700 L/kg DW. The overall maximum sorption was observed at pH between 3 and 4 and the Kd values were significantly higher in unsterilized compared to sterilized samples. The removal of selenite from solution by Pseudomonas sp., Burkholderia sp., Rhodococcus sp. and Paenibacillus sp. strains isolated from the bog was affected by incubation temperature and time. In addition, the incubation of sterilized surface moss, subsurface peat and gyttja samples with added bacteria effectively removed selenite from the solution and on average 65% of selenite was removed when Pseudomonas sp. or Burkholderia sp. strains were used. Our results demonstrate the important role of bacteria for the removal of selenite from the solution phase in the bog environment, having a high organic matter content and a low pH.

AB - 79Se is among the most important long lived radionuclides in spent nuclear fuel and selenite, SeO32-, is its typical form in intermediate redox potential. The sorption behaviour of selenite and the bacterial impact on the selenite sorption in a 7-m-deep profile of a nutrient-poor boreal bog was studied using batch sorption experiments. The batch distribution coefficient (Kd) values of selenite decreased as a function of sampling depth and highest Kd values, 6600 L/kg dry weight (DW), were observed in the surface moss and the lowest in the bottom clay at 1700 L/kg DW. The overall maximum sorption was observed at pH between 3 and 4 and the Kd values were significantly higher in unsterilized compared to sterilized samples. The removal of selenite from solution by Pseudomonas sp., Burkholderia sp., Rhodococcus sp. and Paenibacillus sp. strains isolated from the bog was affected by incubation temperature and time. In addition, the incubation of sterilized surface moss, subsurface peat and gyttja samples with added bacteria effectively removed selenite from the solution and on average 65% of selenite was removed when Pseudomonas sp. or Burkholderia sp. strains were used. Our results demonstrate the important role of bacteria for the removal of selenite from the solution phase in the bog environment, having a high organic matter content and a low pH.

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KW - selenite

KW - peat

KW - bacteria

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