Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 85-96 |
| Journal | Journal of Environmental Radioactivity |
| Volume | 147 |
| DOIs | |
| Publication status | Published - 2015 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- sorption
- biosorption
- selenite
- peat
- bacteria
- nuclear waste
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The microbial impact on the sorption behaviour of selenite in an acidic, nutrient-poor boreal bog. / Lusa, M. (Corresponding Author); Bomberg, M.; Aromaa, H.; Knuutinen, J.; Lehto, J.
In: Journal of Environmental Radioactivity, Vol. 147, 2015, p. 85-96.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - The microbial impact on the sorption behaviour of selenite in an acidic, nutrient-poor boreal bog
AU - Lusa, M.
AU - Bomberg, M.
AU - Aromaa, H.
AU - Knuutinen, J.
AU - Lehto, J.
PY - 2015
Y1 - 2015
N2 - 79Se is among the most important long lived radionuclides in spent nuclear fuel and selenite, SeO32-, is its typical form in intermediate redox potential. The sorption behaviour of selenite and the bacterial impact on the selenite sorption in a 7-m-deep profile of a nutrient-poor boreal bog was studied using batch sorption experiments. The batch distribution coefficient (Kd) values of selenite decreased as a function of sampling depth and highest Kd values, 6600 L/kg dry weight (DW), were observed in the surface moss and the lowest in the bottom clay at 1700 L/kg DW. The overall maximum sorption was observed at pH between 3 and 4 and the Kd values were significantly higher in unsterilized compared to sterilized samples. The removal of selenite from solution by Pseudomonas sp., Burkholderia sp., Rhodococcus sp. and Paenibacillus sp. strains isolated from the bog was affected by incubation temperature and time. In addition, the incubation of sterilized surface moss, subsurface peat and gyttja samples with added bacteria effectively removed selenite from the solution and on average 65% of selenite was removed when Pseudomonas sp. or Burkholderia sp. strains were used. Our results demonstrate the important role of bacteria for the removal of selenite from the solution phase in the bog environment, having a high organic matter content and a low pH.
AB - 79Se is among the most important long lived radionuclides in spent nuclear fuel and selenite, SeO32-, is its typical form in intermediate redox potential. The sorption behaviour of selenite and the bacterial impact on the selenite sorption in a 7-m-deep profile of a nutrient-poor boreal bog was studied using batch sorption experiments. The batch distribution coefficient (Kd) values of selenite decreased as a function of sampling depth and highest Kd values, 6600 L/kg dry weight (DW), were observed in the surface moss and the lowest in the bottom clay at 1700 L/kg DW. The overall maximum sorption was observed at pH between 3 and 4 and the Kd values were significantly higher in unsterilized compared to sterilized samples. The removal of selenite from solution by Pseudomonas sp., Burkholderia sp., Rhodococcus sp. and Paenibacillus sp. strains isolated from the bog was affected by incubation temperature and time. In addition, the incubation of sterilized surface moss, subsurface peat and gyttja samples with added bacteria effectively removed selenite from the solution and on average 65% of selenite was removed when Pseudomonas sp. or Burkholderia sp. strains were used. Our results demonstrate the important role of bacteria for the removal of selenite from the solution phase in the bog environment, having a high organic matter content and a low pH.
KW - sorption
KW - biosorption
KW - selenite
KW - peat
KW - bacteria
KW - nuclear waste
U2 - 10.1016/j.jenvrad.2015.05.014
DO - 10.1016/j.jenvrad.2015.05.014
M3 - Article
VL - 147
SP - 85
EP - 96
JO - Journal of Environmental Radioactivity
JF - Journal of Environmental Radioactivity
SN - 0265-931X
ER -