The missing link to elucidate plant secondary metabolism: combining transcript profiling and targeted metabolome analysis

Research output: Contribution to conferenceConference articleScientific

Abstract

We have developed a technology platform called SOLUCEL to identify the majority of genes involved in the biosynthesis of secondary metabolites and regulation thereof. Furthermore, the genes that enhance the production of secondary metabolites can be functionally screened in a high-throughput mode. We designed this novel approach using tobacco BY-2 cell culture as a model system, in which a cDNA-AFLP based transcript profiling technique is linked with targeted metabolite profiling of these cells to simultaneously identify genes involved in nicotine alkaloid metabolism on a genomewide scale. We have been able to build an ample inventory of genes, either known or novel, involved in tobacco secondary metabolism, and most probably in plant secondary metabolism in general. The great advantage of SOLUCEL technology is its universal application to any plant or cell culture of interest (e.g. rare medicinal plants) without pre-existing gene sequence databases.
Original languageEnglish
Publication statusPublished - 2004
MoE publication typeNot Eligible
Event45th Annual Meeting of the Japanese Society of Plant Physiologists, JSPP - Tokyo, Japan
Duration: 27 Mar 200429 Mar 2004
Conference number: 45

Conference

Conference45th Annual Meeting of the Japanese Society of Plant Physiologists, JSPP
Abbreviated titleJSPP
CountryJapan
CityTokyo
Period27/03/0429/03/04

Fingerprint

metabolomics
metabolism
secondary metabolites
cell culture
tobacco
genes
nicotine
plant cultural practices
amplified fragment length polymorphism
medicinal plants
alkaloids
biosynthesis
metabolites
nucleotide sequences
cells
methodology

Cite this

Inze, D., & Oksman-Caldentey, K-M. (2004). The missing link to elucidate plant secondary metabolism: combining transcript profiling and targeted metabolome analysis. Paper presented at 45th Annual Meeting of the Japanese Society of Plant Physiologists, JSPP, Tokyo, Japan.
Inze, Dirk ; Oksman-Caldentey, Kirsi-Marja. / The missing link to elucidate plant secondary metabolism: combining transcript profiling and targeted metabolome analysis. Paper presented at 45th Annual Meeting of the Japanese Society of Plant Physiologists, JSPP, Tokyo, Japan.
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abstract = "We have developed a technology platform called SOLUCEL to identify the majority of genes involved in the biosynthesis of secondary metabolites and regulation thereof. Furthermore, the genes that enhance the production of secondary metabolites can be functionally screened in a high-throughput mode. We designed this novel approach using tobacco BY-2 cell culture as a model system, in which a cDNA-AFLP based transcript profiling technique is linked with targeted metabolite profiling of these cells to simultaneously identify genes involved in nicotine alkaloid metabolism on a genomewide scale. We have been able to build an ample inventory of genes, either known or novel, involved in tobacco secondary metabolism, and most probably in plant secondary metabolism in general. The great advantage of SOLUCEL technology is its universal application to any plant or cell culture of interest (e.g. rare medicinal plants) without pre-existing gene sequence databases.",
author = "Dirk Inze and Kirsi-Marja Oksman-Caldentey",
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language = "English",
note = "45th Annual Meeting of the Japanese Society of Plant Physiologists, JSPP, JSPP ; Conference date: 27-03-2004 Through 29-03-2004",

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Inze, D & Oksman-Caldentey, K-M 2004, 'The missing link to elucidate plant secondary metabolism: combining transcript profiling and targeted metabolome analysis' Paper presented at 45th Annual Meeting of the Japanese Society of Plant Physiologists, JSPP, Tokyo, Japan, 27/03/04 - 29/03/04, .

The missing link to elucidate plant secondary metabolism: combining transcript profiling and targeted metabolome analysis. / Inze, Dirk; Oksman-Caldentey, Kirsi-Marja.

2004. Paper presented at 45th Annual Meeting of the Japanese Society of Plant Physiologists, JSPP, Tokyo, Japan.

Research output: Contribution to conferenceConference articleScientific

TY - CONF

T1 - The missing link to elucidate plant secondary metabolism: combining transcript profiling and targeted metabolome analysis

AU - Inze, Dirk

AU - Oksman-Caldentey, Kirsi-Marja

PY - 2004

Y1 - 2004

N2 - We have developed a technology platform called SOLUCEL to identify the majority of genes involved in the biosynthesis of secondary metabolites and regulation thereof. Furthermore, the genes that enhance the production of secondary metabolites can be functionally screened in a high-throughput mode. We designed this novel approach using tobacco BY-2 cell culture as a model system, in which a cDNA-AFLP based transcript profiling technique is linked with targeted metabolite profiling of these cells to simultaneously identify genes involved in nicotine alkaloid metabolism on a genomewide scale. We have been able to build an ample inventory of genes, either known or novel, involved in tobacco secondary metabolism, and most probably in plant secondary metabolism in general. The great advantage of SOLUCEL technology is its universal application to any plant or cell culture of interest (e.g. rare medicinal plants) without pre-existing gene sequence databases.

AB - We have developed a technology platform called SOLUCEL to identify the majority of genes involved in the biosynthesis of secondary metabolites and regulation thereof. Furthermore, the genes that enhance the production of secondary metabolites can be functionally screened in a high-throughput mode. We designed this novel approach using tobacco BY-2 cell culture as a model system, in which a cDNA-AFLP based transcript profiling technique is linked with targeted metabolite profiling of these cells to simultaneously identify genes involved in nicotine alkaloid metabolism on a genomewide scale. We have been able to build an ample inventory of genes, either known or novel, involved in tobacco secondary metabolism, and most probably in plant secondary metabolism in general. The great advantage of SOLUCEL technology is its universal application to any plant or cell culture of interest (e.g. rare medicinal plants) without pre-existing gene sequence databases.

M3 - Conference article

ER -

Inze D, Oksman-Caldentey K-M. The missing link to elucidate plant secondary metabolism: combining transcript profiling and targeted metabolome analysis. 2004. Paper presented at 45th Annual Meeting of the Japanese Society of Plant Physiologists, JSPP, Tokyo, Japan.