Abstract
We have developed a technology platform called SOLUCEL to identify the
majority of genes involved in the biosynthesis of secondary metabolites and
regulation thereof. Furthermore, the genes that enhance the production of
secondary metabolites can be functionally screened in a high-throughput mode.
We designed this novel approach using tobacco BY-2 cell culture as a model
system, in which a cDNA-AFLP based transcript profiling technique is linked
with targeted metabolite profiling of these cells to simultaneously identify
genes involved in nicotine alkaloid metabolism on a genomewide scale. We have
been able to build an ample inventory of genes, either known or novel,
involved in tobacco secondary metabolism, and most probably in plant secondary
metabolism in general. The great advantage of SOLUCEL technology is its
universal application to any plant or cell culture of interest (e.g. rare
medicinal plants) without pre-existing gene sequence databases.
| Original language | English |
|---|---|
| Publication status | Published - 2004 |
| MoE publication type | Not Eligible |
| Event | 45th Annual Meeting of the Japanese Society of Plant Physiologists, JSPP - Tokyo, Japan Duration: 27 Mar 2004 → 29 Mar 2004 Conference number: 45 |
Conference
| Conference | 45th Annual Meeting of the Japanese Society of Plant Physiologists, JSPP |
|---|---|
| Abbreviated title | JSPP |
| Country/Territory | Japan |
| City | Tokyo |
| Period | 27/03/04 → 29/03/04 |