The missing link to elucidate plant secondary metabolism: combining transcript profiling and targeted metabolome analysis

Research output: Contribution to conferenceConference articleScientific

Abstract

We have developed a technology platform called SOLUCEL to identify the majority of genes involved in the biosynthesis of secondary metabolites and regulation thereof. Furthermore, the genes that enhance the production of secondary metabolites can be functionally screened in a high-throughput mode. We designed this novel approach using tobacco BY-2 cell culture as a model system, in which a cDNA-AFLP based transcript profiling technique is linked with targeted metabolite profiling of these cells to simultaneously identify genes involved in nicotine alkaloid metabolism on a genomewide scale. We have been able to build an ample inventory of genes, either known or novel, involved in tobacco secondary metabolism, and most probably in plant secondary metabolism in general. The great advantage of SOLUCEL technology is its universal application to any plant or cell culture of interest (e.g. rare medicinal plants) without pre-existing gene sequence databases.
Original languageEnglish
Publication statusPublished - 2004
MoE publication typeNot Eligible
Event45th Annual Meeting of the Japanese Society of Plant Physiologists, JSPP - Tokyo, Japan
Duration: 27 Mar 200429 Mar 2004
Conference number: 45

Conference

Conference45th Annual Meeting of the Japanese Society of Plant Physiologists, JSPP
Abbreviated titleJSPP
CountryJapan
CityTokyo
Period27/03/0429/03/04

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