The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae

Eija Rintala (Corresponding Author), Juha Pekka Pitkänen, Maija Leena Vehkomäki, Merja Penttilä, Laura Ruohonen

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13 Citations (Scopus)

Abstract

The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.

Original languageEnglish
Pages (from-to)129-136
JournalYeast
Volume24
Issue number2
DOIs
Publication statusPublished - 1 Feb 2007
MoE publication typeA1 Journal article-refereed

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Keywords

  • Glyoxylate reductase
  • Hydroxyisocaproate
  • Hydroxypyruvate
  • Saccharomyces cerevisiae

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