TY - JOUR
T1 - The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae
AU - Rintala, Eija
AU - Pitkänen, Juha Pekka
AU - Vehkomäki, Maija Leena
AU - Penttilä, Merja
AU - Ruohonen, Laura
PY - 2007/2/1
Y1 - 2007/2/1
N2 - The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.
AB - The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.
KW - Glyoxylate reductase
KW - Hydroxyisocaproate
KW - Hydroxypyruvate
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=33847248974&partnerID=8YFLogxK
U2 - 10.1002/yea.1434
DO - 10.1002/yea.1434
M3 - Article
C2 - 17173333
AN - SCOPUS:33847248974
VL - 24
SP - 129
EP - 136
JO - Yeast
JF - Yeast
SN - 0749-503X
IS - 2
ER -