Abstract
The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.
| Original language | English |
|---|---|
| Pages (from-to) | 129-136 |
| Journal | Yeast |
| Volume | 24 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Feb 2007 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- Glyoxylate reductase
- Hydroxyisocaproate
- Hydroxypyruvate
- Saccharomyces cerevisiae
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Cite this
Rintala, E., Pitkänen, J. P., Vehkomäki, M. L., Penttilä, M., & Ruohonen, L. (2007). The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae. Yeast, 24(2), 129-136. https://doi.org/10.1002/yea.1434