The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae

Eija Rintala; Juha Pekka Pitkänen; Maija Leena Vehkomäki; Merja Penttilä; Laura Ruohonen

doi:10.1002/yea.1434

The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae

Eija Rintala^*, Juha Pekka Pitkänen, Maija Leena Vehkomäki, Merja Penttilä, Laura Ruohonen

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

19 Citations (Scopus)

Abstract

The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.

Original language	English
Pages (from-to)	129-136
Journal	Yeast
Volume	24
Issue number	2
DOIs	https://doi.org/10.1002/yea.1434
Publication status	Published - 1 Feb 2007
MoE publication type	A1 Journal article-refereed

Keywords

Glyoxylate reductase
Hydroxyisocaproate
Hydroxypyruvate
Saccharomyces cerevisiae

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10.1002/yea.1434

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abstract = "The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.",

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AU - Penttilä, Merja

AU - Ruohonen, Laura

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AB - The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.

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The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae

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Keywords

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