The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae

Eija Rintala (Corresponding Author), Juha Pekka Pitkänen, Maija Leena Vehkomäki, Merja Penttilä, Laura Ruohonen

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.

Original languageEnglish
Pages (from-to)129-136
JournalYeast
Volume24
Issue number2
DOIs
Publication statusPublished - 1 Feb 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

glyoxylate reductase
Yeast
Open Reading Frames
Saccharomyces cerevisiae
open reading frames
glycolic acid
NAD (coenzyme)
enzymes
NADP (coenzyme)
Gene encoding
Enzymes
NADP
NAD
Biomass
biomass
organisms
Oxidoreductases
genes
Genes

Keywords

  • Glyoxylate reductase
  • Hydroxyisocaproate
  • Hydroxypyruvate
  • Saccharomyces cerevisiae

Cite this

Rintala, Eija ; Pitkänen, Juha Pekka ; Vehkomäki, Maija Leena ; Penttilä, Merja ; Ruohonen, Laura. / The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae. In: Yeast. 2007 ; Vol. 24, No. 2. pp. 129-136.
@article{f4ee22e9466c4f7f8ccf48f6413a8e76,
title = "The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae",
abstract = "The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.",
keywords = "Glyoxylate reductase, Hydroxyisocaproate, Hydroxypyruvate, Saccharomyces cerevisiae",
author = "Eija Rintala and Pitk{\"a}nen, {Juha Pekka} and Vehkom{\"a}ki, {Maija Leena} and Merja Penttil{\"a} and Laura Ruohonen",
year = "2007",
month = "2",
day = "1",
doi = "10.1002/yea.1434",
language = "English",
volume = "24",
pages = "129--136",
journal = "Yeast",
issn = "0749-503X",
publisher = "Wiley",
number = "2",

}

The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae. / Rintala, Eija (Corresponding Author); Pitkänen, Juha Pekka; Vehkomäki, Maija Leena; Penttilä, Merja; Ruohonen, Laura.

In: Yeast, Vol. 24, No. 2, 01.02.2007, p. 129-136.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae

AU - Rintala, Eija

AU - Pitkänen, Juha Pekka

AU - Vehkomäki, Maija Leena

AU - Penttilä, Merja

AU - Ruohonen, Laura

PY - 2007/2/1

Y1 - 2007/2/1

N2 - The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.

AB - The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.

KW - Glyoxylate reductase

KW - Hydroxyisocaproate

KW - Hydroxypyruvate

KW - Saccharomyces cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=33847248974&partnerID=8YFLogxK

U2 - 10.1002/yea.1434

DO - 10.1002/yea.1434

M3 - Article

VL - 24

SP - 129

EP - 136

JO - Yeast

JF - Yeast

SN - 0749-503X

IS - 2

ER -