Abstract
The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.
Original language | English |
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Pages (from-to) | 129-136 |
Journal | Yeast |
Volume | 24 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Feb 2007 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Glyoxylate reductase
- Hydroxyisocaproate
- Hydroxypyruvate
- Saccharomyces cerevisiae