The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae

Eija Rintala; Juha Pekka Pitkänen; Maija Leena Vehkomäki; Merja Penttilä; Laura Ruohonen

doi:10.1002/yea.1434

The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae

Eija Rintala^*, Juha Pekka Pitkänen, Maija Leena Vehkomäki, Merja Penttilä, Laura Ruohonen

^*Corresponding author for this work

VTT (former employee or external)

Research output: Contribution to journal › Article › Scientific › peer-review

19 Citations (Scopus)

Abstract

The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.

Original language	English
Pages (from-to)	129-136
Journal	Yeast
Volume	24
Issue number	2
DOIs	https://doi.org/10.1002/yea.1434
Publication status	Published - 1 Feb 2007
MoE publication type	A1 Journal article-refereed

Keywords

Glyoxylate reductase
Hydroxyisocaproate
Hydroxypyruvate
Saccharomyces cerevisiae

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10.1002/yea.1434

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title = "The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae",

abstract = "The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.",

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AU - Rintala, Eija

AU - Pitkänen, Juha Pekka

AU - Vehkomäki, Maija Leena

AU - Penttilä, Merja

AU - Ruohonen, Laura

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N2 - The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.

AB - The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.

KW - Glyoxylate reductase

KW - Hydroxyisocaproate

KW - Hydroxypyruvate

KW - Saccharomyces cerevisiae

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VL - 24

SP - 129

EP - 136

JO - Yeast

JF - Yeast

IS - 2

ER -

The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae

Abstract

Keywords

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