The preliminary characterization of ß-1,4-xylanase of the brown-rot fungus Gloeophyllum trabeum

Anne-Christine Ritschkoff; Marjaana Rättö; Liisa Viikari

The preliminary characterization of ß-1,4-xylanase of the brown-rot fungus Gloeophyllum trabeum

Anne-Christine Ritschkoff, Marjaana Rättö, Liisa Viikari

Research output: Book/Report › Report

Abstract

The extracellular ß-1,4-xylanase of the brown-rot fungus, Gloeophyllum trabeum, was isolated from crude extract by chromatofocusing method (PBE 94 column chromatography). The isoelectric point was estimated to 4.2-4.8 by cromatofocusing and 4.5 by isoelectric focusing (IEF). The molecular weight of the enzyme was estimated to 37,000 dalton by SDS-PAGE. The optimal temperature for the crude extract xylanase was +70°C. The enzyme stability, after 1 h incubation, decreased sharply above +60°C and pH 6.

Original language	English
Place of Publication	Stockholm
Number of pages	4
Publication status	Published - 1990
MoE publication type	D4 Published development or research report or study
Event	International Research Group on Wood Preservation, IRG - Rotorua, New Zealand Duration: 13 May 1990 → 18 May 1990

Publication series

Series	IRG Documents
Number	IRG/WP 1447

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Cite this

@book{af1de66cf35e41afad08f4f98f24a72e,

title = "The preliminary characterization of {\ss}-1,4-xylanase of the brown-rot fungus Gloeophyllum trabeum",

abstract = "The extracellular {\ss}-1,4-xylanase of the brown-rot fungus, Gloeophyllum trabeum, was isolated from crude extract by chromatofocusing method (PBE 94 column chromatography). The isoelectric point was estimated to 4.2-4.8 by cromatofocusing and 4.5 by isoelectric focusing (IEF). The molecular weight of the enzyme was estimated to 37,000 dalton by SDS-PAGE. The optimal temperature for the crude extract xylanase was +70°C. The enzyme stability, after 1 h incubation, decreased sharply above +60°C and pH 6.",

author = "Anne-Christine Ritschkoff and Marjaana R{\"a}tt{\"o} and Liisa Viikari",

year = "1990",

language = "English",

series = "IRG Documents",

number = "IRG/WP 1447",

note = "International Research Group on Wood Preservation, IRG ; Conference date: 13-05-1990 Through 18-05-1990",

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TY - BOOK

T1 - The preliminary characterization of ß-1,4-xylanase of the brown-rot fungus Gloeophyllum trabeum

AU - Ritschkoff, Anne-Christine

AU - Rättö, Marjaana

AU - Viikari, Liisa

PY - 1990

Y1 - 1990

N2 - The extracellular ß-1,4-xylanase of the brown-rot fungus, Gloeophyllum trabeum, was isolated from crude extract by chromatofocusing method (PBE 94 column chromatography). The isoelectric point was estimated to 4.2-4.8 by cromatofocusing and 4.5 by isoelectric focusing (IEF). The molecular weight of the enzyme was estimated to 37,000 dalton by SDS-PAGE. The optimal temperature for the crude extract xylanase was +70°C. The enzyme stability, after 1 h incubation, decreased sharply above +60°C and pH 6.

AB - The extracellular ß-1,4-xylanase of the brown-rot fungus, Gloeophyllum trabeum, was isolated from crude extract by chromatofocusing method (PBE 94 column chromatography). The isoelectric point was estimated to 4.2-4.8 by cromatofocusing and 4.5 by isoelectric focusing (IEF). The molecular weight of the enzyme was estimated to 37,000 dalton by SDS-PAGE. The optimal temperature for the crude extract xylanase was +70°C. The enzyme stability, after 1 h incubation, decreased sharply above +60°C and pH 6.

M3 - Report

T3 - IRG Documents

BT - The preliminary characterization of ß-1,4-xylanase of the brown-rot fungus Gloeophyllum trabeum

CY - Stockholm

T2 - International Research Group on Wood Preservation, IRG

Y2 - 13 May 1990 through 18 May 1990

ER -

The preliminary characterization of ß-1,4-xylanase of the brown-rot fungus Gloeophyllum trabeum

Abstract

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