The extracellular ß-1,4-xylanase of the brown-rot fungus, Gloeophyllum trabeum, was isolated from crude extract by chromatofocusing method (PBE 94 column chromatography). The isoelectric point was estimated to 4.2-4.8 by cromatofocusing and 4.5 by isoelectric focusing (IEF). The molecular weight of the enzyme was estimated to 37,000 dalton by SDS-PAGE. The optimal temperature for the crude extract xylanase was +70°C. The enzyme stability, after 1 h incubation, decreased sharply above +60°C and pH 6.
|Place of Publication||Stockholm|
|Number of pages||4|
|Publication status||Published - 1990|
|MoE publication type||D4 Published development or research report or study|
|Event||International Research Group on Wood Preservation, IRG - Rotorua, New Zealand|
Duration: 13 May 1990 → 18 May 1990