The preliminary characterization of ß-1,4-xylanase of the brown-rot fungus Gloeophyllum trabeum

Anne-Christine Ritschkoff, Marjaana Rättö, Liisa Viikari

Research output: Book/ReportReport

Abstract

The extracellular ß-1,4-xylanase of the brown-rot fungus, Gloeophyllum trabeum, was isolated from crude extract by chromatofocusing method (PBE 94 column chromatography). The isoelectric point was estimated to 4.2-4.8 by cromatofocusing and 4.5 by isoelectric focusing (IEF). The molecular weight of the enzyme was estimated to 37,000 dalton by SDS-PAGE. The optimal temperature for the crude extract xylanase was +70°C. The enzyme stability, after 1 h incubation, decreased sharply above +60°C and pH 6.
Original languageEnglish
Place of PublicationStockholm
Number of pages4
Publication statusPublished - 1990
MoE publication typeD4 Published development or research report or study
EventInternational Research Group on Wood Preservation, IRG - Rotorua, New Zealand
Duration: 13 May 199018 May 1990

Publication series

SeriesIRG Documents
NumberIRG/WP 1447

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