@article{7167ecd5bb9e4e6c86ec3d26e34fe29f,
title = "The quest for fungal strains and their co-culture potential to improve enzymatic degradation of Chinese distillers{\textquoteright} grain and other agricultural wastes",
abstract = "Distillers{\textquoteright} grain (DG) is one of the lignocellulosic agricultural waste streams having a high potential for biofuel production, however it is causing health and environmental problems in Southwest region of China because of improper treatment before being used for landfill. Here, we aimed to identify fungal strains that efficiently hydrolyze DG and explored the possibility to further improve the hydrolysis of DG and other agricultural wastes by co-culturing these fungi. The tested fungal strains included 11 strains that were isolated from DG (Maotai Town, Guizhou, China) and 19 strains that were selected from the CBS collection of Westerdijk Fungal Biodiversity Institute. Aspergillus niger CBS 110.42 and CBS 554.65 and Phanerochaete chrysosporium CBS 246.84 showed overall best cellulose and xylan degrading activity, whereas high laccase and peroxidase activities were detected for Oidiodendron echinulatum CBS 113.65 and Bjerkandera adusta CBS 143380, respectively. Furthermore, A. niger CBS 554.65 co-cultured with Trichoderma reesei CBS 383.78 and A. niger CBS 110.42 with P. chrysosporium CBS 246.84 showed the best improvement (up to 2- and 3-fold higher of β-glucosidase and β-xylosidase activities). These fungi and the combinations have strong potential to be further developed for local bioenergy production. ",
keywords = "Biomass, Co-culture, Distillers' grain, Fungi, Lignocellulose, Saccharification",
author = "Chunyan Ming and Adiphol Dilokpimol and Chenggang Zou and Wanqing Liao and Liang Zhao and Meizhu Wang and {de Vries}, {Ronald P.} and Yingqian Kang",
note = "Funding Information: This work was supported by Guizhou Scientific Plan Project [(2019) 2873]; Excellent Youth Talent Training Project of Guizhou Province [(2017) 5639]; Guiyang Science and Technology Project [(2017) No.5?19]; Talent Base Project of Guizhou Province, China [FCJD2018-22]; Research Fund of Education Bureau of Guizhou Province, China [(2018) 481].The authors wish to thank Tingting Qiu, Hainan University, for supplying the distillers' grain, and Jing Chen, Guizhou Medical University, for depositing the fungi to Chinese academy of Science. Sarah A. Ahmed, Westerdijk Fungal Biodiversity Institute, is thanked for assisting fungal morphological identification. Parastoo Azadi (Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, U.S. Department of Energy grant (DE-SC0015662), Zhirui Wnag and Roberto Sonon, Complex Carbohydrate Research Center, University of Georgia, are thanked for monosaccharide composition and lignin analysis of the distillers' grain. Kelli Griffin, NTrans Technologies BV, is thanked for proofreading the manuscript. Funding Information: This work was supported by Guizhou Scientific Plan Project [ (2019) 2873 ]; Excellent Youth Talent Training Project of Guizhou Province [ (2017) 5639 ]; Guiyang Science and Technology Project [( 2017) No.5–19]; Talent Base Project of Guizhou Province, China [ FCJD2018-22 ]; Research Fund of Education Bureau of Guizhou Province, China [ (2018) 481 ]. Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",
year = "2019",
month = oct,
doi = "10.1016/j.ibiod.2019.104765",
language = "English",
volume = "144",
journal = "International Biodeterioration and Biodegradation",
issn = "0964-8305",
publisher = "Elsevier",
}
