The relation between solution association and surface activity of the hydrophobin HFBI from Trichoderma reesei

Geza Szilvay (Corresponding Author), Kaisa Kisko, Ritva Serimaa, Markus Linder

Research output: Contribution to journalArticleScientificpeer-review

24 Citations (Scopus)

Abstract

Hydrophobins are small fungal surface active proteins that self-assemble at interfaces into films with nanoscale structures. The hydrophobin HFBI from Trichoderma reesei has been shown to associate in solution into tetramers but the role of this association on the function of HFBI has remained unclear. We produced two HFBI variants that showed a significant shift in solution association equilibrium towards the tetramer state. However, this enhanced solution association did not alter the surface properties of the variant HFBIs. The results show that there is not a strong relationship between HFBI solution association state and surface properties such as surface activity.
Original languageEnglish
Pages (from-to)2721-2726
JournalFEBS Letters
Volume581
Issue number14
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma
Surface Properties
Surface properties
Fungal Proteins
Membrane Proteins
1-(heptafluorobutyryl)imidazole
Proteins

Keywords

  • hydrophobins
  • Protein multimerization
  • Protein self-assembly
  • Surface active protein

Cite this

Szilvay, Geza ; Kisko, Kaisa ; Serimaa, Ritva ; Linder, Markus. / The relation between solution association and surface activity of the hydrophobin HFBI from Trichoderma reesei. In: FEBS Letters. 2007 ; Vol. 581, No. 14. pp. 2721-2726.
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The relation between solution association and surface activity of the hydrophobin HFBI from Trichoderma reesei. / Szilvay, Geza (Corresponding Author); Kisko, Kaisa; Serimaa, Ritva; Linder, Markus.

In: FEBS Letters, Vol. 581, No. 14, 2007, p. 2721-2726.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Kisko, Kaisa

AU - Serimaa, Ritva

AU - Linder, Markus

PY - 2007

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AB - Hydrophobins are small fungal surface active proteins that self-assemble at interfaces into films with nanoscale structures. The hydrophobin HFBI from Trichoderma reesei has been shown to associate in solution into tetramers but the role of this association on the function of HFBI has remained unclear. We produced two HFBI variants that showed a significant shift in solution association equilibrium towards the tetramer state. However, this enhanced solution association did not alter the surface properties of the variant HFBIs. The results show that there is not a strong relationship between HFBI solution association state and surface properties such as surface activity.

KW - hydrophobins

KW - Protein multimerization

KW - Protein self-assembly

KW - Surface active protein

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DO - 10.1016/j.febslet.2007.05.024

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