The relation between solution association and surface activity of the hydrophobin HFBI from Trichoderma reesei

Geza Szilvay; Kaisa Kisko; Ritva Serimaa; Markus Linder

doi:10.1016/j.febslet.2007.05.024

The relation between solution association and surface activity of the hydrophobin HFBI from Trichoderma reesei

Geza Szilvay (Corresponding Author), Kaisa Kisko, Ritva Serimaa, Markus Linder

University of Helsinki

Research output: Contribution to journal › Article › Scientific › peer-review

28 Citations (Scopus)

Abstract

Hydrophobins are small fungal surface active proteins that self-assemble at interfaces into films with nanoscale structures. The hydrophobin HFBI from Trichoderma reesei has been shown to associate in solution into tetramers but the role of this association on the function of HFBI has remained unclear. We produced two HFBI variants that showed a significant shift in solution association equilibrium towards the tetramer state. However, this enhanced solution association did not alter the surface properties of the variant HFBIs. The results show that there is not a strong relationship between HFBI solution association state and surface properties such as surface activity.

Original language	English
Pages (from-to)	2721-2726
Journal	FEBS Letters
Volume	581
Issue number	14
DOIs	https://doi.org/10.1016/j.febslet.2007.05.024
Publication status	Published - 2007
MoE publication type	A1 Journal article-refereed

Keywords

hydrophobins
Protein multimerization
Protein self-assembly
Surface active protein

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10.1016/j.febslet.2007.05.024

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title = "The relation between solution association and surface activity of the hydrophobin HFBI from Trichoderma reesei",

abstract = "Hydrophobins are small fungal surface active proteins that self-assemble at interfaces into films with nanoscale structures. The hydrophobin HFBI from Trichoderma reesei has been shown to associate in solution into tetramers but the role of this association on the function of HFBI has remained unclear. We produced two HFBI variants that showed a significant shift in solution association equilibrium towards the tetramer state. However, this enhanced solution association did not alter the surface properties of the variant HFBIs. The results show that there is not a strong relationship between HFBI solution association state and surface properties such as surface activity.",

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T1 - The relation between solution association and surface activity of the hydrophobin HFBI from Trichoderma reesei

AU - Szilvay, Geza

AU - Kisko, Kaisa

AU - Serimaa, Ritva

AU - Linder, Markus

PY - 2007

Y1 - 2007

N2 - Hydrophobins are small fungal surface active proteins that self-assemble at interfaces into films with nanoscale structures. The hydrophobin HFBI from Trichoderma reesei has been shown to associate in solution into tetramers but the role of this association on the function of HFBI has remained unclear. We produced two HFBI variants that showed a significant shift in solution association equilibrium towards the tetramer state. However, this enhanced solution association did not alter the surface properties of the variant HFBIs. The results show that there is not a strong relationship between HFBI solution association state and surface properties such as surface activity.

AB - Hydrophobins are small fungal surface active proteins that self-assemble at interfaces into films with nanoscale structures. The hydrophobin HFBI from Trichoderma reesei has been shown to associate in solution into tetramers but the role of this association on the function of HFBI has remained unclear. We produced two HFBI variants that showed a significant shift in solution association equilibrium towards the tetramer state. However, this enhanced solution association did not alter the surface properties of the variant HFBIs. The results show that there is not a strong relationship between HFBI solution association state and surface properties such as surface activity.

KW - hydrophobins

KW - Protein multimerization

KW - Protein self-assembly

KW - Surface active protein

U2 - 10.1016/j.febslet.2007.05.024

DO - 10.1016/j.febslet.2007.05.024

M3 - Article

SN - 0014-5793

VL - 581

SP - 2721

EP - 2726

JO - FEBS Letters

JF - FEBS Letters

IS - 14

ER -

The relation between solution association and surface activity of the hydrophobin HFBI from Trichoderma reesei

Abstract

Keywords

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