The response of anaerobically grown Saccharomyces cerevisiae to low and high oxygen in glucose-limited chemostat cultures

Marilyn Wiebe, Eija Rintala, Anu Tamminen, Helena Simolin, Laura Salusjärvi, Merja Toivari, Paula Jouhten, Anne Huuskonen, Hannu Maaheimo, Laura Ruohonen, Merja Penttilä

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Saccharomyces cerevisiae was grown anaerobically in glucose-limited chemostat culture for several generations and then exposed to either low (1.0%) or high (20.9%) concentrations of oxygen. Under these conditions the cells required approximately 2 h before starting to take up oxygen or to produce more biomass. Glycerol and ethanol concentrations, began decreasing within an hour of providing oxygen, indicating that they were no longer being produced or were being produced at a slower rate. Little glycerol consumption occurred, but ethanol consumption started after approximately 3 h in cultures receiving 20.9% oxygen. Specific glucose consumption rates decreased as biomass increased in the presence of oxygen. Although the dilution rate was maintained constant at 0.10 h-1, accumulation of biomass in the presence of oxygen indicated that the cells were growing at specific growth rates of 0.21 (1.0% O2) and 0.32 h-1 (20.9% O2) between 2 and 10 h after oxygen was provided to the cultures. Although oxygen uptake did not occur until 2 h after it was provided to the cultures, most glycolytic genes showed down-regulation within 10 minutes of its presence in the culture. Regulation of genes involved in glucose, ethanol and glycerol metabolism and respiration similarly occurred within 10 minutes of the presence of oxygen. In contrast, the concentration of most of the metabolites of central carbon metabolism did not change until more than 10 minutes exposure to oxygen.
Original languageEnglish
Title of host publication3rd European Federation of Biotechnology Conference
Subtitle of host publication Physiology of Yeasts and Filamentous Fungi PYFF3
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages116
ISBN (Electronic)978-951-38-6314-2
ISBN (Print)978-951-38-6313-5
Publication statusPublished - 2007
Event3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi - Helsinki, Finland
Duration: 13 Jun 200716 Jun 2007

Publication series

SeriesVTT Symposium
Number245
ISSN0357-9387

Conference

Conference3rd European Federation of Biotechnology Conference
Abbreviated titlePYFF3
CountryFinland
CityHelsinki
Period13/06/0716/06/07

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    Wiebe, M., Rintala, E., Tamminen, A., Simolin, H., Salusjärvi, L., Toivari, M., Jouhten, P., Huuskonen, A., Maaheimo, H., Ruohonen, L., & Penttilä, M. (2007). The response of anaerobically grown Saccharomyces cerevisiae to low and high oxygen in glucose-limited chemostat cultures. In 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3 (pp. 116). [P69] VTT Technical Research Centre of Finland. VTT Symposium, No. 245 http://www.vtt.fi/inf/pdf/symposiums/2007/S245.pdf