The response of anaerobically grown Saccharomyces cerevisiae to low and high oxygen in glucose-limited chemostat cultures

Marilyn Wiebe, Eija Rintala, Anu Tamminen, Helena Simolin, Laura Salusjärvi, Merja Toivari, Paula Jouhten, Anne Huuskonen, Hannu Maaheimo, Laura Ruohonen, Merja Penttilä

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Saccharomyces cerevisiae was grown anaerobically in glucose-limited chemostat culture for several generations and then exposed to either low (1.0%) or high (20.9%) concentrations of oxygen. Under these conditions the cells required approximately 2 h before starting to take up oxygen or to produce more biomass. Glycerol and ethanol concentrations, began decreasing within an hour of providing oxygen, indicating that they were no longer being produced or were being produced at a slower rate. Little glycerol consumption occurred, but ethanol consumption started after approximately 3 h in cultures receiving 20.9% oxygen. Specific glucose consumption rates decreased as biomass increased in the presence of oxygen. Although the dilution rate was maintained constant at 0.10 h-1, accumulation of biomass in the presence of oxygen indicated that the cells were growing at specific growth rates of 0.21 (1.0% O2) and 0.32 h-1 (20.9% O2) between 2 and 10 h after oxygen was provided to the cultures. Although oxygen uptake did not occur until 2 h after it was provided to the cultures, most glycolytic genes showed down-regulation within 10 minutes of its presence in the culture. Regulation of genes involved in glucose, ethanol and glycerol metabolism and respiration similarly occurred within 10 minutes of the presence of oxygen. In contrast, the concentration of most of the metabolites of central carbon metabolism did not change until more than 10 minutes exposure to oxygen.
Original languageEnglish
Title of host publication3rd European Federation of Biotechnology Conference
Subtitle of host publication Physiology of Yeasts and Filamentous Fungi PYFF3
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages116
ISBN (Electronic)978-951-38-6314-2
ISBN (Print)978-951-38-6313-5
Publication statusPublished - 2007
Event3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi - Helsinki, Finland
Duration: 13 Jun 200716 Jun 2007

Publication series

NameVTT Symposium
PublisherVTT
Number245
ISSN (Print)0357-9387
ISSN (Electronic)1455-0873

Conference

Conference3rd European Federation of Biotechnology Conference
Abbreviated titlePYFF3
CountryFinland
CityHelsinki
Period13/06/0716/06/07

Fingerprint

Saccharomyces cerevisiae
oxygen
glucose
glycerol
ethanol
metabolism
biomass
cell respiration
specific growth rate
biomass production
genes
cells
metabolites
uptake mechanisms
carbon

Cite this

Wiebe, M., Rintala, E., Tamminen, A., Simolin, H., Salusjärvi, L., Toivari, M., ... Penttilä, M. (2007). The response of anaerobically grown Saccharomyces cerevisiae to low and high oxygen in glucose-limited chemostat cultures. In 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3 (pp. 116). [P69] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 245
Wiebe, Marilyn ; Rintala, Eija ; Tamminen, Anu ; Simolin, Helena ; Salusjärvi, Laura ; Toivari, Merja ; Jouhten, Paula ; Huuskonen, Anne ; Maaheimo, Hannu ; Ruohonen, Laura ; Penttilä, Merja. / The response of anaerobically grown Saccharomyces cerevisiae to low and high oxygen in glucose-limited chemostat cultures. 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo : VTT Technical Research Centre of Finland, 2007. pp. 116 (VTT Symposium; No. 245).
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author = "Marilyn Wiebe and Eija Rintala and Anu Tamminen and Helena Simolin and Laura Salusj{\"a}rvi and Merja Toivari and Paula Jouhten and Anne Huuskonen and Hannu Maaheimo and Laura Ruohonen and Merja Penttil{\"a}",
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Wiebe, M, Rintala, E, Tamminen, A, Simolin, H, Salusjärvi, L, Toivari, M, Jouhten, P, Huuskonen, A, Maaheimo, H, Ruohonen, L & Penttilä, M 2007, The response of anaerobically grown Saccharomyces cerevisiae to low and high oxygen in glucose-limited chemostat cultures. in 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3., P69, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 245, pp. 116, 3rd European Federation of Biotechnology Conference , Helsinki, Finland, 13/06/07.

The response of anaerobically grown Saccharomyces cerevisiae to low and high oxygen in glucose-limited chemostat cultures. / Wiebe, Marilyn; Rintala, Eija; Tamminen, Anu; Simolin, Helena; Salusjärvi, Laura; Toivari, Merja; Jouhten, Paula; Huuskonen, Anne; Maaheimo, Hannu; Ruohonen, Laura; Penttilä, Merja.

3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo : VTT Technical Research Centre of Finland, 2007. p. 116 P69 (VTT Symposium; No. 245).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - The response of anaerobically grown Saccharomyces cerevisiae to low and high oxygen in glucose-limited chemostat cultures

AU - Wiebe, Marilyn

AU - Rintala, Eija

AU - Tamminen, Anu

AU - Simolin, Helena

AU - Salusjärvi, Laura

AU - Toivari, Merja

AU - Jouhten, Paula

AU - Huuskonen, Anne

AU - Maaheimo, Hannu

AU - Ruohonen, Laura

AU - Penttilä, Merja

PY - 2007

Y1 - 2007

N2 - Saccharomyces cerevisiae was grown anaerobically in glucose-limited chemostat culture for several generations and then exposed to either low (1.0%) or high (20.9%) concentrations of oxygen. Under these conditions the cells required approximately 2 h before starting to take up oxygen or to produce more biomass. Glycerol and ethanol concentrations, began decreasing within an hour of providing oxygen, indicating that they were no longer being produced or were being produced at a slower rate. Little glycerol consumption occurred, but ethanol consumption started after approximately 3 h in cultures receiving 20.9% oxygen. Specific glucose consumption rates decreased as biomass increased in the presence of oxygen. Although the dilution rate was maintained constant at 0.10 h-1, accumulation of biomass in the presence of oxygen indicated that the cells were growing at specific growth rates of 0.21 (1.0% O2) and 0.32 h-1 (20.9% O2) between 2 and 10 h after oxygen was provided to the cultures. Although oxygen uptake did not occur until 2 h after it was provided to the cultures, most glycolytic genes showed down-regulation within 10 minutes of its presence in the culture. Regulation of genes involved in glucose, ethanol and glycerol metabolism and respiration similarly occurred within 10 minutes of the presence of oxygen. In contrast, the concentration of most of the metabolites of central carbon metabolism did not change until more than 10 minutes exposure to oxygen.

AB - Saccharomyces cerevisiae was grown anaerobically in glucose-limited chemostat culture for several generations and then exposed to either low (1.0%) or high (20.9%) concentrations of oxygen. Under these conditions the cells required approximately 2 h before starting to take up oxygen or to produce more biomass. Glycerol and ethanol concentrations, began decreasing within an hour of providing oxygen, indicating that they were no longer being produced or were being produced at a slower rate. Little glycerol consumption occurred, but ethanol consumption started after approximately 3 h in cultures receiving 20.9% oxygen. Specific glucose consumption rates decreased as biomass increased in the presence of oxygen. Although the dilution rate was maintained constant at 0.10 h-1, accumulation of biomass in the presence of oxygen indicated that the cells were growing at specific growth rates of 0.21 (1.0% O2) and 0.32 h-1 (20.9% O2) between 2 and 10 h after oxygen was provided to the cultures. Although oxygen uptake did not occur until 2 h after it was provided to the cultures, most glycolytic genes showed down-regulation within 10 minutes of its presence in the culture. Regulation of genes involved in glucose, ethanol and glycerol metabolism and respiration similarly occurred within 10 minutes of the presence of oxygen. In contrast, the concentration of most of the metabolites of central carbon metabolism did not change until more than 10 minutes exposure to oxygen.

M3 - Conference abstract in proceedings

SN - 978-951-38-6313-5

T3 - VTT Symposium

SP - 116

BT - 3rd European Federation of Biotechnology Conference

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Wiebe M, Rintala E, Tamminen A, Simolin H, Salusjärvi L, Toivari M et al. The response of anaerobically grown Saccharomyces cerevisiae to low and high oxygen in glucose-limited chemostat cultures. In 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo: VTT Technical Research Centre of Finland. 2007. p. 116. P69. (VTT Symposium; No. 245).