The role of CRE1 in nucleosome positioning within the cbh1 promoter and coding regions of Trichoderma reesei

L. Ries, N. J. Belshaw, M. Ilmén, M. E. Penttilä, M. Alapuranen, D. B. Archer

Research output: Contribution to journalArticleScientificpeer-review

31 Citations (Scopus)

Abstract

Nucleosome positioning within the promoter and coding regions of the cellobiohydrolase-encoding cbh1 gene of Trichoderma reesei was investigated. T. reesei is a filamentous fungus that is able to degrade dead plant biomass by secreting enzymes such as cellulases, a feature which is exploited in industrial applications. In the presence of different carbon sources, regulation of one of these cellulase-encoding genes, cbh1, is mediated by various transcription factors including CRE1. Deletion or mutation of cre1 caused an increase in cbh1 transcript levels under repressing conditions. CRE1 was shown to bind to several consensus recognition sequences in the cbh1 promoter region in vitro. Under repressing conditions (glucose), the cbh1 promoter and coding regions are occupied by several positioned nucleosomes. Transcription of cbh1 in the presence of the inducer sophorose resulted in a loss of nucleosomes from the coding region and in the re-positioning of the promoter nucleosomes which prevents CRE1 from binding to its recognition sites within the promoter region. Strains expressing a non-functional CRE1 (in strains with mutated CRE1 or cre1-deletion) exhibited a loss of positioned nucleosomes within the cbh1 coding region under repressing conditions only. This indicates that CRE1 is important for correct nucleosome positioning within the cbh1 coding region under repressing conditions.

Original languageEnglish
Pages (from-to)749-762
Number of pages14
JournalApplied Microbiology and Biotechnology
Volume98
Issue number2
DOIs
Publication statusPublished - 1 Jan 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma
Nucleosomes
Genetic Promoter Regions
Cellulose 1,4-beta-Cellobiosidase
Cellulases
Cellulase
Sequence Deletion
Consensus Sequence
Biomass
Genes
Fungi
Transcription Factors
Carbon
Glucose
Enzymes

Keywords

  • Cellobiohydrolase I
  • Cellobiohydrolase II
  • CRE1
  • Nucleosome positioning
  • Trichoderma reesei

Cite this

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title = "The role of CRE1 in nucleosome positioning within the cbh1 promoter and coding regions of Trichoderma reesei",
abstract = "Nucleosome positioning within the promoter and coding regions of the cellobiohydrolase-encoding cbh1 gene of Trichoderma reesei was investigated. T. reesei is a filamentous fungus that is able to degrade dead plant biomass by secreting enzymes such as cellulases, a feature which is exploited in industrial applications. In the presence of different carbon sources, regulation of one of these cellulase-encoding genes, cbh1, is mediated by various transcription factors including CRE1. Deletion or mutation of cre1 caused an increase in cbh1 transcript levels under repressing conditions. CRE1 was shown to bind to several consensus recognition sequences in the cbh1 promoter region in vitro. Under repressing conditions (glucose), the cbh1 promoter and coding regions are occupied by several positioned nucleosomes. Transcription of cbh1 in the presence of the inducer sophorose resulted in a loss of nucleosomes from the coding region and in the re-positioning of the promoter nucleosomes which prevents CRE1 from binding to its recognition sites within the promoter region. Strains expressing a non-functional CRE1 (in strains with mutated CRE1 or cre1-deletion) exhibited a loss of positioned nucleosomes within the cbh1 coding region under repressing conditions only. This indicates that CRE1 is important for correct nucleosome positioning within the cbh1 coding region under repressing conditions.",
keywords = "Cellobiohydrolase I, Cellobiohydrolase II, CRE1, Nucleosome positioning, Trichoderma reesei",
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The role of CRE1 in nucleosome positioning within the cbh1 promoter and coding regions of Trichoderma reesei. / Ries, L.; Belshaw, N. J.; Ilmén, M.; Penttilä, M. E.; Alapuranen, M.; Archer, D. B.

In: Applied Microbiology and Biotechnology, Vol. 98, No. 2, 01.01.2014, p. 749-762.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The role of CRE1 in nucleosome positioning within the cbh1 promoter and coding regions of Trichoderma reesei

AU - Ries, L.

AU - Belshaw, N. J.

AU - Ilmén, M.

AU - Penttilä, M. E.

AU - Alapuranen, M.

AU - Archer, D. B.

N1 - CA2: BA3111 CA2: BA311 SDA: BIC ISI: BIOTECHNOLOGY & APPLIED MICROBIOLOGY

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Nucleosome positioning within the promoter and coding regions of the cellobiohydrolase-encoding cbh1 gene of Trichoderma reesei was investigated. T. reesei is a filamentous fungus that is able to degrade dead plant biomass by secreting enzymes such as cellulases, a feature which is exploited in industrial applications. In the presence of different carbon sources, regulation of one of these cellulase-encoding genes, cbh1, is mediated by various transcription factors including CRE1. Deletion or mutation of cre1 caused an increase in cbh1 transcript levels under repressing conditions. CRE1 was shown to bind to several consensus recognition sequences in the cbh1 promoter region in vitro. Under repressing conditions (glucose), the cbh1 promoter and coding regions are occupied by several positioned nucleosomes. Transcription of cbh1 in the presence of the inducer sophorose resulted in a loss of nucleosomes from the coding region and in the re-positioning of the promoter nucleosomes which prevents CRE1 from binding to its recognition sites within the promoter region. Strains expressing a non-functional CRE1 (in strains with mutated CRE1 or cre1-deletion) exhibited a loss of positioned nucleosomes within the cbh1 coding region under repressing conditions only. This indicates that CRE1 is important for correct nucleosome positioning within the cbh1 coding region under repressing conditions.

AB - Nucleosome positioning within the promoter and coding regions of the cellobiohydrolase-encoding cbh1 gene of Trichoderma reesei was investigated. T. reesei is a filamentous fungus that is able to degrade dead plant biomass by secreting enzymes such as cellulases, a feature which is exploited in industrial applications. In the presence of different carbon sources, regulation of one of these cellulase-encoding genes, cbh1, is mediated by various transcription factors including CRE1. Deletion or mutation of cre1 caused an increase in cbh1 transcript levels under repressing conditions. CRE1 was shown to bind to several consensus recognition sequences in the cbh1 promoter region in vitro. Under repressing conditions (glucose), the cbh1 promoter and coding regions are occupied by several positioned nucleosomes. Transcription of cbh1 in the presence of the inducer sophorose resulted in a loss of nucleosomes from the coding region and in the re-positioning of the promoter nucleosomes which prevents CRE1 from binding to its recognition sites within the promoter region. Strains expressing a non-functional CRE1 (in strains with mutated CRE1 or cre1-deletion) exhibited a loss of positioned nucleosomes within the cbh1 coding region under repressing conditions only. This indicates that CRE1 is important for correct nucleosome positioning within the cbh1 coding region under repressing conditions.

KW - Cellobiohydrolase I

KW - Cellobiohydrolase II

KW - CRE1

KW - Nucleosome positioning

KW - Trichoderma reesei

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M3 - Article

VL - 98

SP - 749

EP - 762

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 2

ER -