The role of endoglucanase and endoxylanase in liquefaction of hydrothermally pretreated wheat straw

P A Skovgaard (Corresponding Author), L G Thygesen, H Jørgensen, M Cardona, E Tozzi, M Mccarthy, Matti Siika-aho

Research output: Contribution to journalArticleScientificpeer-review

15 Citations (Scopus)

Abstract

The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield
Original languageEnglish
Pages (from-to)923-931
Number of pages8
JournalBiotechnology Progress
Volume30
Issue number4
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

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Endo-1,4-beta Xylanases
Cellulase
Viscosity
Triticum
Cellulases
Magnetic Resonance Imaging

Keywords

  • Endoglucanase
  • endoxylanase
  • fiber attrition
  • magnetic resonance imaging
  • thermostable enzmes
  • viscosity

Cite this

Skovgaard, P. A., Thygesen, L. G., Jørgensen, H., Cardona, M., Tozzi, E., Mccarthy, M., & Siika-aho, M. (2014). The role of endoglucanase and endoxylanase in liquefaction of hydrothermally pretreated wheat straw. Biotechnology Progress, 30(4), 923-931. https://doi.org/10.1002/btpr.1893
Skovgaard, P A ; Thygesen, L G ; Jørgensen, H ; Cardona, M ; Tozzi, E ; Mccarthy, M ; Siika-aho, Matti. / The role of endoglucanase and endoxylanase in liquefaction of hydrothermally pretreated wheat straw. In: Biotechnology Progress. 2014 ; Vol. 30, No. 4. pp. 923-931.
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abstract = "The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6{\%} total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield",
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Skovgaard, PA, Thygesen, LG, Jørgensen, H, Cardona, M, Tozzi, E, Mccarthy, M & Siika-aho, M 2014, 'The role of endoglucanase and endoxylanase in liquefaction of hydrothermally pretreated wheat straw', Biotechnology Progress, vol. 30, no. 4, pp. 923-931. https://doi.org/10.1002/btpr.1893

The role of endoglucanase and endoxylanase in liquefaction of hydrothermally pretreated wheat straw. / Skovgaard, P A (Corresponding Author); Thygesen, L G; Jørgensen, H; Cardona, M; Tozzi, E; Mccarthy, M; Siika-aho, Matti.

In: Biotechnology Progress, Vol. 30, No. 4, 2014, p. 923-931.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Skovgaard, P A

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AU - Tozzi, E

AU - Mccarthy, M

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AB - The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield

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KW - endoxylanase

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KW - viscosity

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Skovgaard PA, Thygesen LG, Jørgensen H, Cardona M, Tozzi E, Mccarthy M et al. The role of endoglucanase and endoxylanase in liquefaction of hydrothermally pretreated wheat straw. Biotechnology Progress. 2014;30(4):923-931. https://doi.org/10.1002/btpr.1893