The role of endoglucanase and endoxylanase in liquefaction of hydrothermally pretreated wheat straw

P A Skovgaard (Corresponding Author), L G Thygesen, H Jørgensen, M Cardona, E Tozzi, M Mccarthy, Matti Siika-aho

    Research output: Contribution to journalArticleScientificpeer-review

    18 Citations (Scopus)

    Abstract

    The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield
    Original languageEnglish
    Pages (from-to)923-931
    Number of pages8
    JournalBiotechnology Progress
    Volume30
    Issue number4
    DOIs
    Publication statusPublished - 2014
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Endoglucanase
    • endoxylanase
    • fiber attrition
    • magnetic resonance imaging
    • thermostable enzmes
    • viscosity

    Fingerprint Dive into the research topics of 'The role of endoglucanase and endoxylanase in liquefaction of hydrothermally pretreated wheat straw'. Together they form a unique fingerprint.

    Cite this