The role of phosphorylation in redox regulation of photosynthesis genes psaA and psbA during photosynthetic acclimation of mustard

S. Steiner, L. Dietzel, Y. Schroter, Vidal Fey, R. Wagner, T. Pfannschmidt (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

43 Citations (Scopus)

Abstract

The long-term response (LTR) to light-quality gradients improves performance and survival of plants in dense stands. It involves redox-controlled transcriptional regulation of the plastome-encoded genes psaAB (encoding the P700 apoproteins of photosystem I) and psbA (encoding the D1 protein of photosystem II) and requires the action of plastid-localized kinases. To study the potential impact of phosphorylation events on plastid gene expression during the LTR, we analyzed mustard seedlings acclimated to light sources favoring either photosystem I or photosystem II. Primer extension analyses of psaA transcripts indicate that the redox regulation occurs at the principal bacterial promoters, suggesting that the plastid encoded RNA polymerase (PEP) is the target for redox signals. Chloroplast protein fractions containing PEP and other DNA-binding proteins were purified from mustard via heparin-Sepharose chromatography. The biochemical properties of these fractions were analyzed with special emphasis on promoter recognition and specificity, phosphorylation state, and kinase activity. The results demonstrate that the LTR involves the action of small DNA-binding proteins; three of them exhibit specific changes in the phosphorylation state. Auto-phosphorylation assays, in addition, exhibit large differences in the activity of endogenous kinase activities. Chloroplast run-on transcription experiments with the kinase inhibitor H7 and the reductant DTT indicate that phosphorylation events are essential for the mediation of redox signals toward psaA and psbA transcription initiation, but require the synergistic action of a thiol redox signal. The data support the idea that redox signals from the thylakoid membrane are linked to gene expression via phosphorylation events; however, this mediation appears to require a complex network of interacting proteins rather than a simple phosphorelay.
Original languageEnglish
Pages (from-to)416-429
JournalMolecular Plant
Volume2
Issue number3
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Mustard Plant
Acclimatization
Photosynthesis
Oxidation-Reduction
acclimation
phosphorylation
Plastids
Phosphorylation
plastids
photosynthesis
phosphotransferases (kinases)
Phosphotransferases
DNA-binding proteins
Genes
photosystem I
Photosystem I Protein Complex
DNA-directed RNA polymerase
Photosystem II Protein Complex
genes
photosystem II

Keywords

  • Light-quality acclimation
  • redox control
  • protein phosphorylation
  • chloroplast transcription
  • Sinapis alba

Cite this

Steiner, S. ; Dietzel, L. ; Schroter, Y. ; Fey, Vidal ; Wagner, R. ; Pfannschmidt, T. / The role of phosphorylation in redox regulation of photosynthesis genes psaA and psbA during photosynthetic acclimation of mustard. In: Molecular Plant. 2009 ; Vol. 2, No. 3. pp. 416-429.
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The role of phosphorylation in redox regulation of photosynthesis genes psaA and psbA during photosynthetic acclimation of mustard. / Steiner, S.; Dietzel, L.; Schroter, Y.; Fey, Vidal; Wagner, R.; Pfannschmidt, T. (Corresponding Author).

In: Molecular Plant, Vol. 2, No. 3, 2009, p. 416-429.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - The role of phosphorylation in redox regulation of photosynthesis genes psaA and psbA during photosynthetic acclimation of mustard

AU - Steiner, S.

AU - Dietzel, L.

AU - Schroter, Y.

AU - Fey, Vidal

AU - Wagner, R.

AU - Pfannschmidt, T.

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AB - The long-term response (LTR) to light-quality gradients improves performance and survival of plants in dense stands. It involves redox-controlled transcriptional regulation of the plastome-encoded genes psaAB (encoding the P700 apoproteins of photosystem I) and psbA (encoding the D1 protein of photosystem II) and requires the action of plastid-localized kinases. To study the potential impact of phosphorylation events on plastid gene expression during the LTR, we analyzed mustard seedlings acclimated to light sources favoring either photosystem I or photosystem II. Primer extension analyses of psaA transcripts indicate that the redox regulation occurs at the principal bacterial promoters, suggesting that the plastid encoded RNA polymerase (PEP) is the target for redox signals. Chloroplast protein fractions containing PEP and other DNA-binding proteins were purified from mustard via heparin-Sepharose chromatography. The biochemical properties of these fractions were analyzed with special emphasis on promoter recognition and specificity, phosphorylation state, and kinase activity. The results demonstrate that the LTR involves the action of small DNA-binding proteins; three of them exhibit specific changes in the phosphorylation state. Auto-phosphorylation assays, in addition, exhibit large differences in the activity of endogenous kinase activities. Chloroplast run-on transcription experiments with the kinase inhibitor H7 and the reductant DTT indicate that phosphorylation events are essential for the mediation of redox signals toward psaA and psbA transcription initiation, but require the synergistic action of a thiol redox signal. The data support the idea that redox signals from the thylakoid membrane are linked to gene expression via phosphorylation events; however, this mediation appears to require a complex network of interacting proteins rather than a simple phosphorelay.

KW - Light-quality acclimation

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KW - protein phosphorylation

KW - chloroplast transcription

KW - Sinapis alba

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M3 - Article

VL - 2

SP - 416

EP - 429

JO - Molecular Plant

JF - Molecular Plant

SN - 1674-2052

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