The role of TRPV6 in breast carcinogenesis

Katrin A. Bolanz, Matthias A. Hediger, Christopher P. Landowski

Research output: Contribution to journalArticleScientificpeer-review

104 Citations (Scopus)

Abstract

TRPV6 is an endothelial calcium entry channel that is strongly expressed in breast adenocarcinoma tissue. In this study, we further confirmed this observation by analysis of breast cancer tissues, which indicated that TRPV6 mRNA expression was up-regulated between 2-fold and 15-fold compared with the average in normal breast tissue. Whereas TRPV6 is expressed in the cancer tissue, its role as a calcium channel in breast carcinogenesis is poorly understood. Therefore, we investigated how TRPV6 affects the viability, apoptosis, and calcium transport in the breast cancer cell line T47D. Hormones can also affect the tumor development; hence, we determined the effects of estradiol, progesterone, and 1,25-vitamin D on TRPV6 transcription. Interestingly, the estrogen receptor antagonist tamoxifen reduced expression of TRPV6 and is able to inhibit its calcium transport activity (IC50, 7.5 μmol/L). The in vitro model showed that TRPV6 can be regulated by estrogen, progesterone, tamoxifen, and 1,25-vitamin D and has a large influence on breast cancer cell proliferation. Moreover, the effect of tamoxifen on cell viability was enhanced when TRPV6 expression was silenced with small interfering RNA. TRPV6 may be a novel target for the development of calcium channel inhibitors to treat breast adenocarcinoma expressing TRPV6.

Original languageEnglish
Pages (from-to)271-279
Number of pages9
JournalMolecular Cancer Therapeutics
Volume7
Issue number2
DOIs
Publication statusPublished - 1 Feb 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Carcinogenesis
Breast
Tamoxifen
Calcium Channels
Breast Neoplasms
Vitamin D
Progesterone
Adenocarcinoma
Calcium
Small Interfering RNA
Inhibitory Concentration 50
Estradiol
Neoplasms
Cell Survival
Estrogens
Cell Proliferation
Hormones
Apoptosis
Cell Line
Messenger RNA

Cite this

Bolanz, Katrin A. ; Hediger, Matthias A. ; Landowski, Christopher P. / The role of TRPV6 in breast carcinogenesis. In: Molecular Cancer Therapeutics. 2008 ; Vol. 7, No. 2. pp. 271-279.
@article{833a699c8c964927a346fd35e69cd0a5,
title = "The role of TRPV6 in breast carcinogenesis",
abstract = "TRPV6 is an endothelial calcium entry channel that is strongly expressed in breast adenocarcinoma tissue. In this study, we further confirmed this observation by analysis of breast cancer tissues, which indicated that TRPV6 mRNA expression was up-regulated between 2-fold and 15-fold compared with the average in normal breast tissue. Whereas TRPV6 is expressed in the cancer tissue, its role as a calcium channel in breast carcinogenesis is poorly understood. Therefore, we investigated how TRPV6 affects the viability, apoptosis, and calcium transport in the breast cancer cell line T47D. Hormones can also affect the tumor development; hence, we determined the effects of estradiol, progesterone, and 1,25-vitamin D on TRPV6 transcription. Interestingly, the estrogen receptor antagonist tamoxifen reduced expression of TRPV6 and is able to inhibit its calcium transport activity (IC50, 7.5 μmol/L). The in vitro model showed that TRPV6 can be regulated by estrogen, progesterone, tamoxifen, and 1,25-vitamin D and has a large influence on breast cancer cell proliferation. Moreover, the effect of tamoxifen on cell viability was enhanced when TRPV6 expression was silenced with small interfering RNA. TRPV6 may be a novel target for the development of calcium channel inhibitors to treat breast adenocarcinoma expressing TRPV6.",
author = "Bolanz, {Katrin A.} and Hediger, {Matthias A.} and Landowski, {Christopher P.}",
year = "2008",
month = "2",
day = "1",
doi = "10.1158/1535-7163.MCT-07-0478",
language = "English",
volume = "7",
pages = "271--279",
journal = "Molecular Cancer Therapeutics",
issn = "1535-7163",
publisher = "American Association for Cancer Research Inc.",
number = "2",

}

The role of TRPV6 in breast carcinogenesis. / Bolanz, Katrin A.; Hediger, Matthias A.; Landowski, Christopher P.

In: Molecular Cancer Therapeutics, Vol. 7, No. 2, 01.02.2008, p. 271-279.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The role of TRPV6 in breast carcinogenesis

AU - Bolanz, Katrin A.

AU - Hediger, Matthias A.

AU - Landowski, Christopher P.

PY - 2008/2/1

Y1 - 2008/2/1

N2 - TRPV6 is an endothelial calcium entry channel that is strongly expressed in breast adenocarcinoma tissue. In this study, we further confirmed this observation by analysis of breast cancer tissues, which indicated that TRPV6 mRNA expression was up-regulated between 2-fold and 15-fold compared with the average in normal breast tissue. Whereas TRPV6 is expressed in the cancer tissue, its role as a calcium channel in breast carcinogenesis is poorly understood. Therefore, we investigated how TRPV6 affects the viability, apoptosis, and calcium transport in the breast cancer cell line T47D. Hormones can also affect the tumor development; hence, we determined the effects of estradiol, progesterone, and 1,25-vitamin D on TRPV6 transcription. Interestingly, the estrogen receptor antagonist tamoxifen reduced expression of TRPV6 and is able to inhibit its calcium transport activity (IC50, 7.5 μmol/L). The in vitro model showed that TRPV6 can be regulated by estrogen, progesterone, tamoxifen, and 1,25-vitamin D and has a large influence on breast cancer cell proliferation. Moreover, the effect of tamoxifen on cell viability was enhanced when TRPV6 expression was silenced with small interfering RNA. TRPV6 may be a novel target for the development of calcium channel inhibitors to treat breast adenocarcinoma expressing TRPV6.

AB - TRPV6 is an endothelial calcium entry channel that is strongly expressed in breast adenocarcinoma tissue. In this study, we further confirmed this observation by analysis of breast cancer tissues, which indicated that TRPV6 mRNA expression was up-regulated between 2-fold and 15-fold compared with the average in normal breast tissue. Whereas TRPV6 is expressed in the cancer tissue, its role as a calcium channel in breast carcinogenesis is poorly understood. Therefore, we investigated how TRPV6 affects the viability, apoptosis, and calcium transport in the breast cancer cell line T47D. Hormones can also affect the tumor development; hence, we determined the effects of estradiol, progesterone, and 1,25-vitamin D on TRPV6 transcription. Interestingly, the estrogen receptor antagonist tamoxifen reduced expression of TRPV6 and is able to inhibit its calcium transport activity (IC50, 7.5 μmol/L). The in vitro model showed that TRPV6 can be regulated by estrogen, progesterone, tamoxifen, and 1,25-vitamin D and has a large influence on breast cancer cell proliferation. Moreover, the effect of tamoxifen on cell viability was enhanced when TRPV6 expression was silenced with small interfering RNA. TRPV6 may be a novel target for the development of calcium channel inhibitors to treat breast adenocarcinoma expressing TRPV6.

UR - http://www.scopus.com/inward/record.url?scp=39749123226&partnerID=8YFLogxK

U2 - 10.1158/1535-7163.MCT-07-0478

DO - 10.1158/1535-7163.MCT-07-0478

M3 - Article

C2 - 18245667

AN - SCOPUS:39749123226

VL - 7

SP - 271

EP - 279

JO - Molecular Cancer Therapeutics

JF - Molecular Cancer Therapeutics

SN - 1535-7163

IS - 2

ER -