The role of xylem class III peroxidases in lignification

Kaisa Marjamaa, Eija M. Kukkola, Kurt V. Fagerstedt (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

92 Citations (Scopus)

Abstract

Lignification is a cell wall fortifying process which occurs in xylem tissue in a scheduled manner during tissue differentiation. In this review, enzymes and the genes responsible for lignin biosynthesis have been studied with an emphasis on lignin polymerizing class III secretable plant peroxidases. Our aim is to understand the cell and molecular biology of the polymerization of lignin especially in tracheids and vessels of woody species but much of the experimental evidence comes from herbaceous plants. Class III peroxidases pose many problems for empirical work as their encoding genes are variable, their substrate specificities are wide and the half-life of many of the isozymes is very long. However, there is some evidence for the role of specific peroxidases in lignin polymerization through antisense mutants in tobacco and poplar and from tissue and cell culture lines of Picea abies and Zinnia elegans. Peroxidase enzyme action has been shown by substrate specificity studies and, for example, RT-PCR results have pointed out that many peroxidases have tissue-specific expression patterns. Tissue-level location of gene expression of some peroxidases has been studied by in situ hybridization and their cellular localization with antibodies and using EGFP-fusion genes. From these, it can be concluded that, although many of the xylem class III peroxidases have the potential for functioning in the synthesis of the lignin polymer, the combined information of catalytic properties, expression, and localization can reveal differences in the significance of different peroxidases in the lignification process.
Original languageEnglish
Pages (from-to)367-376
Number of pages10
JournalJournal of Experimental Botany
Volume60
Issue number2
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Peroxidases
Xylem
lignification
peroxidases
xylem
Lignin
lignin
polymerization
substrate specificity
Substrate Specificity
Polymerization
lignin peroxidase
Zinnia violacea
Abies
Picea
tracheids
gene fusion
Gene Fusion
herbaceous plants
xylem vessels

Keywords

  • Cell wall
  • lignin
  • peroxidase
  • polymerization

Cite this

Marjamaa, Kaisa ; Kukkola, Eija M. ; Fagerstedt, Kurt V. / The role of xylem class III peroxidases in lignification. In: Journal of Experimental Botany. 2009 ; Vol. 60, No. 2. pp. 367-376.
@article{9eb5abf4ffc4459e9cf2b0f744b41747,
title = "The role of xylem class III peroxidases in lignification",
abstract = "Lignification is a cell wall fortifying process which occurs in xylem tissue in a scheduled manner during tissue differentiation. In this review, enzymes and the genes responsible for lignin biosynthesis have been studied with an emphasis on lignin polymerizing class III secretable plant peroxidases. Our aim is to understand the cell and molecular biology of the polymerization of lignin especially in tracheids and vessels of woody species but much of the experimental evidence comes from herbaceous plants. Class III peroxidases pose many problems for empirical work as their encoding genes are variable, their substrate specificities are wide and the half-life of many of the isozymes is very long. However, there is some evidence for the role of specific peroxidases in lignin polymerization through antisense mutants in tobacco and poplar and from tissue and cell culture lines of Picea abies and Zinnia elegans. Peroxidase enzyme action has been shown by substrate specificity studies and, for example, RT-PCR results have pointed out that many peroxidases have tissue-specific expression patterns. Tissue-level location of gene expression of some peroxidases has been studied by in situ hybridization and their cellular localization with antibodies and using EGFP-fusion genes. From these, it can be concluded that, although many of the xylem class III peroxidases have the potential for functioning in the synthesis of the lignin polymer, the combined information of catalytic properties, expression, and localization can reveal differences in the significance of different peroxidases in the lignification process.",
keywords = "Cell wall, lignin, peroxidase, polymerization",
author = "Kaisa Marjamaa and Kukkola, {Eija M.} and Fagerstedt, {Kurt V.}",
year = "2009",
doi = "10.1093/jxb/ern278",
language = "English",
volume = "60",
pages = "367--376",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "2",

}

The role of xylem class III peroxidases in lignification. / Marjamaa, Kaisa; Kukkola, Eija M.; Fagerstedt, Kurt V. (Corresponding Author).

In: Journal of Experimental Botany, Vol. 60, No. 2, 2009, p. 367-376.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The role of xylem class III peroxidases in lignification

AU - Marjamaa, Kaisa

AU - Kukkola, Eija M.

AU - Fagerstedt, Kurt V.

PY - 2009

Y1 - 2009

N2 - Lignification is a cell wall fortifying process which occurs in xylem tissue in a scheduled manner during tissue differentiation. In this review, enzymes and the genes responsible for lignin biosynthesis have been studied with an emphasis on lignin polymerizing class III secretable plant peroxidases. Our aim is to understand the cell and molecular biology of the polymerization of lignin especially in tracheids and vessels of woody species but much of the experimental evidence comes from herbaceous plants. Class III peroxidases pose many problems for empirical work as their encoding genes are variable, their substrate specificities are wide and the half-life of many of the isozymes is very long. However, there is some evidence for the role of specific peroxidases in lignin polymerization through antisense mutants in tobacco and poplar and from tissue and cell culture lines of Picea abies and Zinnia elegans. Peroxidase enzyme action has been shown by substrate specificity studies and, for example, RT-PCR results have pointed out that many peroxidases have tissue-specific expression patterns. Tissue-level location of gene expression of some peroxidases has been studied by in situ hybridization and their cellular localization with antibodies and using EGFP-fusion genes. From these, it can be concluded that, although many of the xylem class III peroxidases have the potential for functioning in the synthesis of the lignin polymer, the combined information of catalytic properties, expression, and localization can reveal differences in the significance of different peroxidases in the lignification process.

AB - Lignification is a cell wall fortifying process which occurs in xylem tissue in a scheduled manner during tissue differentiation. In this review, enzymes and the genes responsible for lignin biosynthesis have been studied with an emphasis on lignin polymerizing class III secretable plant peroxidases. Our aim is to understand the cell and molecular biology of the polymerization of lignin especially in tracheids and vessels of woody species but much of the experimental evidence comes from herbaceous plants. Class III peroxidases pose many problems for empirical work as their encoding genes are variable, their substrate specificities are wide and the half-life of many of the isozymes is very long. However, there is some evidence for the role of specific peroxidases in lignin polymerization through antisense mutants in tobacco and poplar and from tissue and cell culture lines of Picea abies and Zinnia elegans. Peroxidase enzyme action has been shown by substrate specificity studies and, for example, RT-PCR results have pointed out that many peroxidases have tissue-specific expression patterns. Tissue-level location of gene expression of some peroxidases has been studied by in situ hybridization and their cellular localization with antibodies and using EGFP-fusion genes. From these, it can be concluded that, although many of the xylem class III peroxidases have the potential for functioning in the synthesis of the lignin polymer, the combined information of catalytic properties, expression, and localization can reveal differences in the significance of different peroxidases in the lignification process.

KW - Cell wall

KW - lignin

KW - peroxidase

KW - polymerization

U2 - 10.1093/jxb/ern278

DO - 10.1093/jxb/ern278

M3 - Article

VL - 60

SP - 367

EP - 376

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 2

ER -