The role of xylonolactone in xylonic acid production by Pseudomonas fragi

Johanna Buchert, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

25 Citations (Scopus)

Abstract

Xylonic acid was produced by Pseudomonas fragi ATCC 4973 on 15% xylose medium with a yield of 96% of the theoretical. In the middle of the fermentation, growth was inhibited due to formation of inhibitory xylono-γ-lactone.
The spontaneous, non-enzymatic hydrolysis of lactone accounted for only 19% of the hydrolysis demand, thus explaining the accumulation of xylonolactone in the broth.
It appeared that high amounts of xylono-γ-lactone induced xylonolactonase enzyme, which brought about the total hydrolysis of xylonolactone.
Original languageEnglish
Pages (from-to)333-336
JournalApplied Microbiology and Biotechnology
Volume27
Issue number4
DOIs
Publication statusPublished - 1988
MoE publication typeA1 Journal article-refereed

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Pseudomonas fragi
Lactones
Hydrolysis
Acids
Xylose
Fermentation
Enzymes
Growth

Cite this

Buchert, Johanna ; Viikari, Liisa. / The role of xylonolactone in xylonic acid production by Pseudomonas fragi. In: Applied Microbiology and Biotechnology. 1988 ; Vol. 27, No. 4. pp. 333-336.
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The role of xylonolactone in xylonic acid production by Pseudomonas fragi. / Buchert, Johanna; Viikari, Liisa.

In: Applied Microbiology and Biotechnology, Vol. 27, No. 4, 1988, p. 333-336.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The role of xylonolactone in xylonic acid production by Pseudomonas fragi

AU - Buchert, Johanna

AU - Viikari, Liisa

PY - 1988

Y1 - 1988

N2 - Xylonic acid was produced by Pseudomonas fragi ATCC 4973 on 15% xylose medium with a yield of 96% of the theoretical. In the middle of the fermentation, growth was inhibited due to formation of inhibitory xylono-γ-lactone. The spontaneous, non-enzymatic hydrolysis of lactone accounted for only 19% of the hydrolysis demand, thus explaining the accumulation of xylonolactone in the broth. It appeared that high amounts of xylono-γ-lactone induced xylonolactonase enzyme, which brought about the total hydrolysis of xylonolactone.

AB - Xylonic acid was produced by Pseudomonas fragi ATCC 4973 on 15% xylose medium with a yield of 96% of the theoretical. In the middle of the fermentation, growth was inhibited due to formation of inhibitory xylono-γ-lactone. The spontaneous, non-enzymatic hydrolysis of lactone accounted for only 19% of the hydrolysis demand, thus explaining the accumulation of xylonolactone in the broth. It appeared that high amounts of xylono-γ-lactone induced xylonolactonase enzyme, which brought about the total hydrolysis of xylonolactone.

U2 - 10.1007/BF00251763

DO - 10.1007/BF00251763

M3 - Article

VL - 27

SP - 333

EP - 336

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 4

ER -