Xylonic acid was produced by Pseudomonas fragi ATCC 4973 on 15% xylose medium with a yield of 96% of the theoretical. In the middle of the fermentation, growth was inhibited due to formation of inhibitory xylono-γ-lactone. The spontaneous, non-enzymatic hydrolysis of lactone accounted for only 19% of the hydrolysis demand, thus explaining the accumulation of xylonolactone in the broth. It appeared that high amounts of xylono-γ-lactone induced xylonolactonase enzyme, which brought about the total hydrolysis of xylonolactone.
|Journal||Applied Microbiology and Biotechnology|
|Publication status||Published - 1988|
|MoE publication type||A1 Journal article-refereed|