The role of xylulokinase in Saccharomyces cerevisiae xylulose catabolism

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75 Citations (Scopus)

Abstract

Many yeast species have growth rates on D-xylulose of 25-130% of those on glucose, but for Saccharomyces cerevisiae this ratio is only about 6%. The xylulokinase reaction has been proposed to be the rate-limiting step in the D-xylulose fermentation with S. cerevisiae. Over-expression of xylulokinase encoding XKS1 stimulated growth on D-xylulose in a S. cerevisiae strain to about 20% of the growth rate on glucose and deletion of the gene prevented growth on D-xylulose and D-xylulose metabolism. We have partially purified the xylulokinase and characterised its kinetic properties. It is reversible and will also accept D-ribulose as a substrate. (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalFEMS Microbiology Letters
Volume190
Issue number1
DOIs
Publication statusPublished - 1 Sep 2000
MoE publication typeA1 Journal article-refereed

Fingerprint

Xylulose
Saccharomyces cerevisiae
Growth
Glucose
Gene Deletion
Fermentation
Yeasts
xylulokinase

Keywords

  • D-Ribulose
  • D-Xylulose
  • Saccharomyces cerevisiae
  • Xylose fermentation
  • Xylulokinase

Cite this

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abstract = "Many yeast species have growth rates on D-xylulose of 25-130{\%} of those on glucose, but for Saccharomyces cerevisiae this ratio is only about 6{\%}. The xylulokinase reaction has been proposed to be the rate-limiting step in the D-xylulose fermentation with S. cerevisiae. Over-expression of xylulokinase encoding XKS1 stimulated growth on D-xylulose in a S. cerevisiae strain to about 20{\%} of the growth rate on glucose and deletion of the gene prevented growth on D-xylulose and D-xylulose metabolism. We have partially purified the xylulokinase and characterised its kinetic properties. It is reversible and will also accept D-ribulose as a substrate. (C) 2000 Federation of European Microbiological Societies.",
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The role of xylulokinase in Saccharomyces cerevisiae xylulose catabolism. / Richard, Peter; Toivari, Mervi H.; Penttilä, Merja.

In: FEMS Microbiology Letters, Vol. 190, No. 1, 01.09.2000, p. 39-43.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The role of xylulokinase in Saccharomyces cerevisiae xylulose catabolism

AU - Richard, Peter

AU - Toivari, Mervi H.

AU - Penttilä, Merja

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N2 - Many yeast species have growth rates on D-xylulose of 25-130% of those on glucose, but for Saccharomyces cerevisiae this ratio is only about 6%. The xylulokinase reaction has been proposed to be the rate-limiting step in the D-xylulose fermentation with S. cerevisiae. Over-expression of xylulokinase encoding XKS1 stimulated growth on D-xylulose in a S. cerevisiae strain to about 20% of the growth rate on glucose and deletion of the gene prevented growth on D-xylulose and D-xylulose metabolism. We have partially purified the xylulokinase and characterised its kinetic properties. It is reversible and will also accept D-ribulose as a substrate. (C) 2000 Federation of European Microbiological Societies.

AB - Many yeast species have growth rates on D-xylulose of 25-130% of those on glucose, but for Saccharomyces cerevisiae this ratio is only about 6%. The xylulokinase reaction has been proposed to be the rate-limiting step in the D-xylulose fermentation with S. cerevisiae. Over-expression of xylulokinase encoding XKS1 stimulated growth on D-xylulose in a S. cerevisiae strain to about 20% of the growth rate on glucose and deletion of the gene prevented growth on D-xylulose and D-xylulose metabolism. We have partially purified the xylulokinase and characterised its kinetic properties. It is reversible and will also accept D-ribulose as a substrate. (C) 2000 Federation of European Microbiological Societies.

KW - D-Ribulose

KW - D-Xylulose

KW - Saccharomyces cerevisiae

KW - Xylose fermentation

KW - Xylulokinase

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