The role of xylulokinase in Saccharomyces cerevisiae xylulose catabolism

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Many yeast species have growth rates on D-xylulose of 25-130% of those on glucose, but for Saccharomyces cerevisiae this ratio is only about 6%. The xylulokinase reaction has been proposed to be the rate-limiting step in the D-xylulose fermentation with S. cerevisiae. Over-expression of xylulokinase encoding XKS1 stimulated growth on D-xylulose in a S. cerevisiae strain to about 20% of the growth rate on glucose and deletion of the gene prevented growth on D-xylulose and D-xylulose metabolism. We have partially purified the xylulokinase and characterised its kinetic properties. It is reversible and will also accept D-ribulose as a substrate. (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalFEMS Microbiology Letters
Issue number1
Publication statusPublished - 1 Sep 2000
MoE publication typeA1 Journal article-refereed


  • D-Ribulose
  • D-Xylulose
  • Saccharomyces cerevisiae
  • Xylose fermentation
  • Xylulokinase

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