The role of xylulokinase in Saccharomyces cerevisiae xylulose catabolism

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    Many yeast species have growth rates on D-xylulose of 25-130% of those on glucose, but for Saccharomyces cerevisiae this ratio is only about 6%. The xylulokinase reaction has been proposed to be the rate-limiting step in the D-xylulose fermentation with S. cerevisiae. Over-expression of xylulokinase encoding XKS1 stimulated growth on D-xylulose in a S. cerevisiae strain to about 20% of the growth rate on glucose and deletion of the gene prevented growth on D-xylulose and D-xylulose metabolism. We have partially purified the xylulokinase and characterised its kinetic properties. It is reversible and will also accept D-ribulose as a substrate. (C) 2000 Federation of European Microbiological Societies.

    Original languageEnglish
    Pages (from-to)39-43
    Number of pages5
    JournalFEMS Microbiology Letters
    Issue number1
    Publication statusPublished - 1 Sept 2000
    MoE publication typeA1 Journal article-refereed


    • D-Ribulose
    • D-Xylulose
    • Saccharomyces cerevisiae
    • Xylose fermentation
    • Xylulokinase


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