Punahometta aiheuttavan Fusarium-sienen seriiniproteinaasit ja niiden inhibiittorit

Translated title of the contribution: The serine proteinases of Fusarium and their inhibition by barley proteins

Anja Pekkarinen

Research output: Contribution to journalArticleProfessional

Abstract

Fusarium head blight (FHB, scab) of cereals is a serious, world-wide problem for producers and industries that use grain as raw material. Scabby grain processes poorly and the toxins that are produced by the fungi pose potential health risks to humans and animals. Understanding the interactions between the fungus and the plant would help in developing more resistant cereal cultivars that also have good agronomical and malting qualities. Proteolytic enzymes may be among the tools that the fungus use to attack the spikes. The purpose of this dissertation was to pinpoint and characterize the proteinases that are synthesized by Fusarium species to degrade grain proteins during infection and to identify any barley proteins that can inhibit those enzymes. The proteinases, a subtilisin-like (SL) and a trypsin-like (TL) enzyme, were shown to degrade barley storage proteins, C and D group hordeins. This implied that they are involved in increasing free amino nitrogen (FAN) and soluble protein levels in malt. The grain proteins that inhibited the SL enzyme were barley alfa-amylase/subtilisin inhibitor (BASI) and chymotrypsin/subtilisin inhibitors 1A, 1B and 2A. The TL enzyme was inhibited by barley Bowman-Birk inhibitor (BBBI). The roles that these proteinases and their inhibitors may play during the infection of cereal spikes by Fusaria were discussed in the dissertation, but more research is needed to establish their true purposes.
Original languageFinnish
Pages (from-to)37-41
JournalMallas ja Olut
Issue number2
Publication statusPublished - 2003
MoE publication typeD1 Article in a trade journal

Fingerprint

barley protein
serine proteinases
subtilisin
Fusarium
enzymes
proteinases
grain protein
trypsin
fungi
inflorescences
Bowman-Birk inhibitor
barley
malting quality
amino nitrogen
Fusarium head blight
malt
chymotrypsin
storage proteins
alpha-amylase
infection

Keywords

  • scab
  • Fusarium
  • proteinases
  • barley
  • cereals

Cite this

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title = "Punahometta aiheuttavan Fusarium-sienen seriiniproteinaasit ja niiden inhibiittorit",
abstract = "Fusarium head blight (FHB, scab) of cereals is a serious, world-wide problem for producers and industries that use grain as raw material. Scabby grain processes poorly and the toxins that are produced by the fungi pose potential health risks to humans and animals. Understanding the interactions between the fungus and the plant would help in developing more resistant cereal cultivars that also have good agronomical and malting qualities. Proteolytic enzymes may be among the tools that the fungus use to attack the spikes. The purpose of this dissertation was to pinpoint and characterize the proteinases that are synthesized by Fusarium species to degrade grain proteins during infection and to identify any barley proteins that can inhibit those enzymes. The proteinases, a subtilisin-like (SL) and a trypsin-like (TL) enzyme, were shown to degrade barley storage proteins, C and D group hordeins. This implied that they are involved in increasing free amino nitrogen (FAN) and soluble protein levels in malt. The grain proteins that inhibited the SL enzyme were barley alfa-amylase/subtilisin inhibitor (BASI) and chymotrypsin/subtilisin inhibitors 1A, 1B and 2A. The TL enzyme was inhibited by barley Bowman-Birk inhibitor (BBBI). The roles that these proteinases and their inhibitors may play during the infection of cereal spikes by Fusaria were discussed in the dissertation, but more research is needed to establish their true purposes.",
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author = "Anja Pekkarinen",
year = "2003",
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Punahometta aiheuttavan Fusarium-sienen seriiniproteinaasit ja niiden inhibiittorit. / Pekkarinen, Anja.

In: Mallas ja Olut, No. 2 , 2003, p. 37-41.

Research output: Contribution to journalArticleProfessional

TY - JOUR

T1 - Punahometta aiheuttavan Fusarium-sienen seriiniproteinaasit ja niiden inhibiittorit

AU - Pekkarinen, Anja

PY - 2003

Y1 - 2003

N2 - Fusarium head blight (FHB, scab) of cereals is a serious, world-wide problem for producers and industries that use grain as raw material. Scabby grain processes poorly and the toxins that are produced by the fungi pose potential health risks to humans and animals. Understanding the interactions between the fungus and the plant would help in developing more resistant cereal cultivars that also have good agronomical and malting qualities. Proteolytic enzymes may be among the tools that the fungus use to attack the spikes. The purpose of this dissertation was to pinpoint and characterize the proteinases that are synthesized by Fusarium species to degrade grain proteins during infection and to identify any barley proteins that can inhibit those enzymes. The proteinases, a subtilisin-like (SL) and a trypsin-like (TL) enzyme, were shown to degrade barley storage proteins, C and D group hordeins. This implied that they are involved in increasing free amino nitrogen (FAN) and soluble protein levels in malt. The grain proteins that inhibited the SL enzyme were barley alfa-amylase/subtilisin inhibitor (BASI) and chymotrypsin/subtilisin inhibitors 1A, 1B and 2A. The TL enzyme was inhibited by barley Bowman-Birk inhibitor (BBBI). The roles that these proteinases and their inhibitors may play during the infection of cereal spikes by Fusaria were discussed in the dissertation, but more research is needed to establish their true purposes.

AB - Fusarium head blight (FHB, scab) of cereals is a serious, world-wide problem for producers and industries that use grain as raw material. Scabby grain processes poorly and the toxins that are produced by the fungi pose potential health risks to humans and animals. Understanding the interactions between the fungus and the plant would help in developing more resistant cereal cultivars that also have good agronomical and malting qualities. Proteolytic enzymes may be among the tools that the fungus use to attack the spikes. The purpose of this dissertation was to pinpoint and characterize the proteinases that are synthesized by Fusarium species to degrade grain proteins during infection and to identify any barley proteins that can inhibit those enzymes. The proteinases, a subtilisin-like (SL) and a trypsin-like (TL) enzyme, were shown to degrade barley storage proteins, C and D group hordeins. This implied that they are involved in increasing free amino nitrogen (FAN) and soluble protein levels in malt. The grain proteins that inhibited the SL enzyme were barley alfa-amylase/subtilisin inhibitor (BASI) and chymotrypsin/subtilisin inhibitors 1A, 1B and 2A. The TL enzyme was inhibited by barley Bowman-Birk inhibitor (BBBI). The roles that these proteinases and their inhibitors may play during the infection of cereal spikes by Fusaria were discussed in the dissertation, but more research is needed to establish their true purposes.

KW - scab

KW - Fusarium

KW - proteinases

KW - barley

KW - cereals

M3 - Article

SP - 37

EP - 41

JO - Mallas ja Olut

JF - Mallas ja Olut

SN - 0356-3014

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ER -