The synthetic potential of fungal feruloyl esterases: A correlation with current classification systems and predicted structural properties

Io Antonopoulou, Adiphol Dilokpimol, Laura Iancu, Miia R. Mäkelä, Simona Varriale, Gabriella Cerullo, Silvia Hüttner, Stefan Uthoff, Peter Jütten, Alexander Piechot, Alexander Steinbüchel, Lisbeth Olsson, Vincenza Faraco, Kristiina S. Hildén, Ronald P. de Vries, Ulrika Rova, Paul Christakopoulos

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

Twenty-eight fungal feruloyl esterases (FAEs) were evaluated for their synthetic abilities in a ternary system of n-hexane: t-butanol: 100 mM MOPS-NaOH pH 6.0 forming detergentless microemulsions. Five main derivatives were synthesized, namely prenyl ferulate, prenyl caffeate, butyl ferulate, glyceryl ferulate, and L-arabinose ferulate, offering, in general, higher yields when more hydrophilic alcohol substitutions were used. Acetyl xylan esterase-related FAEs belonging to phylogenetic subfamilies (SF) 5 and 6 showed increased synthetic yields among tested enzymes. In particular, it was shown that FAEs belonging to SF6 generally transesterified aliphatic alcohols more efficiently while SF5 members preferred bulkier L-arabinose. Predicted surface properties and structural characteristics were correlated with the synthetic potential of selected tannase-related, acetyl-xylan-related, and lipase-related FAEs (SF1-2,-6,-7 members) based on homology modeling and small molecular docking simulations.

Original languageEnglish
Article number242
JournalCatalysts
Volume8
Issue number6
DOIs
Publication statusPublished - 7 Jun 2018
MoE publication typeA1 Journal article-refereed

Keywords

  • Antioxidants
  • Butyl ferulate
  • Feruloyl esterase
  • Glyceryl ferulate
  • L-arabinose ferulate
  • Phylogenetic classification
  • Prenyl caffeate
  • Prenyl ferulate
  • Sugar esters
  • Transesterification

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