The 'true' l-xylulose reductase of filamentous fungi identified in Aspergillus niger

Research output: Contribution to journalArticleScientificpeer-review

22 Citations (Scopus)

Abstract

l-Xylulose reductase is part of the eukaryotic pathway for l-arabinose catabolism. A previously identified l-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on l-arabinose and the deletion strain showed no reduced l-xylulose reductase activity but instead lost the d-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' l-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on l-arabinose and the deletion results in a strain lacking the NADPH-specific l-xylulose reductase activity and having reduced growth on l-arabinose. The purified enzyme had a Km for l-xylulose of 25mM and a νmax of 650U/mg.

Original languageEnglish
Pages (from-to)3540-3544
JournalFEBS Letters
Volume584
Issue number16
DOIs
Publication statusPublished - 1 Aug 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Xylulose
Aspergillus niger
Aspergillus
Fungi
Oxidoreductases
Arabinose
Mannitol Dehydrogenases
Hypocrea
Growth
NADP
Genes
Communication
Enzymes

Keywords

  • Aspergillus niger
  • EC 1.1.1.10
  • L-Arabinose metabolism
  • L-Xylulose
  • Pentose

Cite this

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abstract = "l-Xylulose reductase is part of the eukaryotic pathway for l-arabinose catabolism. A previously identified l-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on l-arabinose and the deletion strain showed no reduced l-xylulose reductase activity but instead lost the d-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' l-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on l-arabinose and the deletion results in a strain lacking the NADPH-specific l-xylulose reductase activity and having reduced growth on l-arabinose. The purified enzyme had a Km for l-xylulose of 25mM and a νmax of 650U/mg.",
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The 'true' l-xylulose reductase of filamentous fungi identified in Aspergillus niger. / Mojzita, Dominik; Vuoristo, Kiira; Koivistoinen, Outi M.; Penttilä, Merja; Richard, Peter.

In: FEBS Letters, Vol. 584, No. 16, 01.08.2010, p. 3540-3544.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Vuoristo, Kiira

AU - Koivistoinen, Outi M.

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AU - Richard, Peter

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