The 'true' l-xylulose reductase of filamentous fungi identified in Aspergillus niger

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Abstract

l-Xylulose reductase is part of the eukaryotic pathway for l-arabinose catabolism. A previously identified l-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on l-arabinose and the deletion strain showed no reduced l-xylulose reductase activity but instead lost the d-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' l-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on l-arabinose and the deletion results in a strain lacking the NADPH-specific l-xylulose reductase activity and having reduced growth on l-arabinose. The purified enzyme had a Km for l-xylulose of 25mM and a νmax of 650U/mg.

Original languageEnglish
Pages (from-to)3540-3544
JournalFEBS Letters
Volume584
Issue number16
DOIs
Publication statusPublished - 1 Aug 2010
MoE publication typeA1 Journal article-refereed

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Keywords

  • Aspergillus niger
  • EC 1.1.1.10
  • L-Arabinose metabolism
  • L-Xylulose
  • Pentose

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