The 'true' l-xylulose reductase of filamentous fungi identified in Aspergillus niger

    Research output: Contribution to journalArticleScientificpeer-review

    25 Citations (Scopus)

    Abstract

    l-Xylulose reductase is part of the eukaryotic pathway for l-arabinose catabolism. A previously identified l-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on l-arabinose and the deletion strain showed no reduced l-xylulose reductase activity but instead lost the d-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' l-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on l-arabinose and the deletion results in a strain lacking the NADPH-specific l-xylulose reductase activity and having reduced growth on l-arabinose. The purified enzyme had a Km for l-xylulose of 25mM and a νmax of 650U/mg.

    Original languageEnglish
    Pages (from-to)3540-3544
    JournalFEBS Letters
    Volume584
    Issue number16
    DOIs
    Publication statusPublished - 1 Aug 2010
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Aspergillus niger
    • EC 1.1.1.10
    • L-Arabinose metabolism
    • L-Xylulose
    • Pentose

    Fingerprint

    Dive into the research topics of 'The 'true' l-xylulose reductase of filamentous fungi identified in Aspergillus niger'. Together they form a unique fingerprint.

    Cite this