The 'true' l-xylulose reductase of filamentous fungi identified in Aspergillus niger

Dominik Mojzita; Kiira Vuoristo; Outi M. Koivistoinen; Merja Penttilä; Peter Richard

doi:10.1016/j.febslet.2010.06.037

The 'true' l-xylulose reductase of filamentous fungi identified in Aspergillus niger

^*Corresponding author for this work

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33 Citations (Scopus)

Abstract

l-Xylulose reductase is part of the eukaryotic pathway for l-arabinose catabolism. A previously identified l-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on l-arabinose and the deletion strain showed no reduced l-xylulose reductase activity but instead lost the d-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' l-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on l-arabinose and the deletion results in a strain lacking the NADPH-specific l-xylulose reductase activity and having reduced growth on l-arabinose. The purified enzyme had a K_m for l-xylulose of 25mM and a ν_max of 650U/mg.

Original language	English
Pages (from-to)	3540-3544
Journal	FEBS Letters
Volume	584
Issue number	16
DOIs	https://doi.org/10.1016/j.febslet.2010.06.037
Publication status	Published - 1 Aug 2010
MoE publication type	A1 Journal article-refereed

Funding

This work was financially supported by the Academy of Finland through the research programme “White Biotechnology – Green Chemistry”, The Finnish Centre of Excellence Programme, 2008–2013, Project No. 118573 and an Academy Research Fellowship for P.R. We thank Anne Usvalampi for the l -xylulose producing E. coli strain.

Keywords

Aspergillus niger
EC 1.1.1.10
L-Arabinose metabolism
L-Xylulose
Pentose

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10.1016/j.febslet.2010.06.037

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abstract = "l-Xylulose reductase is part of the eukaryotic pathway for l-arabinose catabolism. A previously identified l-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on l-arabinose and the deletion strain showed no reduced l-xylulose reductase activity but instead lost the d-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' l-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on l-arabinose and the deletion results in a strain lacking the NADPH-specific l-xylulose reductase activity and having reduced growth on l-arabinose. The purified enzyme had a Km for l-xylulose of 25mM and a νmax of 650U/mg.",

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AU - Koivistoinen, Outi M.

AU - Penttilä, Merja

AU - Richard, Peter

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AB - l-Xylulose reductase is part of the eukaryotic pathway for l-arabinose catabolism. A previously identified l-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on l-arabinose and the deletion strain showed no reduced l-xylulose reductase activity but instead lost the d-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' l-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on l-arabinose and the deletion results in a strain lacking the NADPH-specific l-xylulose reductase activity and having reduced growth on l-arabinose. The purified enzyme had a Km for l-xylulose of 25mM and a νmax of 650U/mg.

KW - Aspergillus niger

KW - EC 1.1.1.10

KW - L-Arabinose metabolism

KW - L-Xylulose

KW - Pentose

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ER -

The 'true' l-xylulose reductase of filamentous fungi identified in Aspergillus niger

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Keywords

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