The use of Photobacterium leiognathi in studies of process equipment cleanability

Gun Wirtanen (Corresponding Author), Vesa Nissinen, Leena Tikkanen, Tiina Mattila-Sandholm

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)

Abstract

The aim of the EC funded Project AIRI‐CT‐92 is to develop methods for the cleanability testing of food processing equipment. Photobacterium leiognathi ATCC 33469 has been suggested as an indicator organism in cleanability testing. Here, this bacterium was grown as a biofilm for 5 h or 24 h at room temperature on various surfaces, clean or milk‐soiled, by immersing the surfaces in luminous medium inoculated with P. leiognathi. The surfaces were then rinsed or cleaned using a CIP procedure before the hygienic status was determined using conventional plating, bioluminescence output and image analysis.

Rinsing did not eliminate the bacterium from the surfaces nor did the CIP procedure when 24‐h biofilms were used, as was readily seen after enrichment in luminous medium. In image analysis the biofilm showed a spotlike growth pattern which was most evident on stainless steel. The advantage of P. leiognathi in the cleanability assessment, which is based on the natural light output of the test bacterium, is that the test is easy to perform and can be carried out without use of expensive reagents.
Original languageEnglish
Pages (from-to)523-533
JournalInternational Journal of Food Science and Technology
Volume30
Issue number4
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

Fingerprint

Photobacterium leiognathi
Photobacterium
Biofilms
biofilm
Bacteria
Equipment and Supplies
Image analysis
bacteria
Food Handling
food processing equipment
Stainless Steel
testing
image analysis
Bioluminescence
Food processing
bioluminescence
Testing
stainless steel
Plating
indicator species

Cite this

Wirtanen, Gun ; Nissinen, Vesa ; Tikkanen, Leena ; Mattila-Sandholm, Tiina. / The use of Photobacterium leiognathi in studies of process equipment cleanability. In: International Journal of Food Science and Technology. 1995 ; Vol. 30, No. 4. pp. 523-533.
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abstract = "The aim of the EC funded Project AIRI‐CT‐92 is to develop methods for the cleanability testing of food processing equipment. Photobacterium leiognathi ATCC 33469 has been suggested as an indicator organism in cleanability testing. Here, this bacterium was grown as a biofilm for 5 h or 24 h at room temperature on various surfaces, clean or milk‐soiled, by immersing the surfaces in luminous medium inoculated with P. leiognathi. The surfaces were then rinsed or cleaned using a CIP procedure before the hygienic status was determined using conventional plating, bioluminescence output and image analysis.Rinsing did not eliminate the bacterium from the surfaces nor did the CIP procedure when 24‐h biofilms were used, as was readily seen after enrichment in luminous medium. In image analysis the biofilm showed a spotlike growth pattern which was most evident on stainless steel. The advantage of P. leiognathi in the cleanability assessment, which is based on the natural light output of the test bacterium, is that the test is easy to perform and can be carried out without use of expensive reagents.",
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The use of Photobacterium leiognathi in studies of process equipment cleanability. / Wirtanen, Gun (Corresponding Author); Nissinen, Vesa; Tikkanen, Leena; Mattila-Sandholm, Tiina.

In: International Journal of Food Science and Technology, Vol. 30, No. 4, 1995, p. 523-533.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The use of Photobacterium leiognathi in studies of process equipment cleanability

AU - Wirtanen, Gun

AU - Nissinen, Vesa

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AU - Mattila-Sandholm, Tiina

N1 - Project code: BEL2823

PY - 1995

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N2 - The aim of the EC funded Project AIRI‐CT‐92 is to develop methods for the cleanability testing of food processing equipment. Photobacterium leiognathi ATCC 33469 has been suggested as an indicator organism in cleanability testing. Here, this bacterium was grown as a biofilm for 5 h or 24 h at room temperature on various surfaces, clean or milk‐soiled, by immersing the surfaces in luminous medium inoculated with P. leiognathi. The surfaces were then rinsed or cleaned using a CIP procedure before the hygienic status was determined using conventional plating, bioluminescence output and image analysis.Rinsing did not eliminate the bacterium from the surfaces nor did the CIP procedure when 24‐h biofilms were used, as was readily seen after enrichment in luminous medium. In image analysis the biofilm showed a spotlike growth pattern which was most evident on stainless steel. The advantage of P. leiognathi in the cleanability assessment, which is based on the natural light output of the test bacterium, is that the test is easy to perform and can be carried out without use of expensive reagents.

AB - The aim of the EC funded Project AIRI‐CT‐92 is to develop methods for the cleanability testing of food processing equipment. Photobacterium leiognathi ATCC 33469 has been suggested as an indicator organism in cleanability testing. Here, this bacterium was grown as a biofilm for 5 h or 24 h at room temperature on various surfaces, clean or milk‐soiled, by immersing the surfaces in luminous medium inoculated with P. leiognathi. The surfaces were then rinsed or cleaned using a CIP procedure before the hygienic status was determined using conventional plating, bioluminescence output and image analysis.Rinsing did not eliminate the bacterium from the surfaces nor did the CIP procedure when 24‐h biofilms were used, as was readily seen after enrichment in luminous medium. In image analysis the biofilm showed a spotlike growth pattern which was most evident on stainless steel. The advantage of P. leiognathi in the cleanability assessment, which is based on the natural light output of the test bacterium, is that the test is easy to perform and can be carried out without use of expensive reagents.

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DO - 10.1111/j.1365-2621.1995.tb01399.x

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JO - International Journal of Food Science and Technology

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