Three cellulases from Melanocarpus albomyces for textile treatment at neutral pH

Arja Miettinen-Oinonen (Corresponding Author), John Londesborough, Vesa Joutsjoki, Raija Lantto, Jari Vehmaanperä

Research output: Contribution to journalArticleScientificpeer-review

83 Citations (Scopus)

Abstract

Culture supernatants from strains of Melanocarpus albomyces, Myceliophthora thermophila, Chaetomium thermophilum, and Sporotrichum thermophilum were tested for their ability to release dye in neutral pH conditions from indigo-dyed cotton-containing fabric in biostoning applications. The supernatants from M. albomyces worked well in biostoning, with low backstaining. Three cellulases were purified to homogeneity from the culture medium of this species. Two of the cellulases were endoglucanases with apparent molecular masses of 20 and 50 kDa. The 20 kDa endoglucanase was a relatively heat-stable cellulase with high pH optimum. The partially purified enzyme crystallized spontaneously at pH 4.0 and 7 °C. The 50 kDa endoglucanase also had activity against 4-methylumbelliferyl-β-d-lactoside (MUL) and was active over a wide range of pH values. The third purified cellulase was the 50 kDa cellobiohydrolase with low MUL activity at acidic pH and detectable activity towards filter paper and acid swollen Solca Floc-cellulose, but no endoglucanase activity. The purified 20 kDa endoglucanase performed well in biostoning of denim fabric at neutral pH. Addition of the purified 50 kDa endoglucanase or the 50 kDa cellobiohydrolase to the 20 kDa endoglucanase decreased backstaining in biostoning.
Original languageEnglish
Pages (from-to)332 - 341
Number of pages10
JournalEnzyme and Microbial Technology
Volume34
Issue number3-4
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

Cellulases
Cellulase
Textiles
Molecular mass
Cotton
Cellulose
Enzymes
Dyes
Cellulose 1,4-beta-Cellobiosidase
Acids
Chaetomium
Sporothrix
Indigo Carmine
Culture Media
Coloring Agents
Hot Temperature

Keywords

  • Melanocarpus albomyces
  • Textile treatment
  • Neutral cellulase

Cite this

Miettinen-Oinonen, Arja ; Londesborough, John ; Joutsjoki, Vesa ; Lantto, Raija ; Vehmaanperä, Jari. / Three cellulases from Melanocarpus albomyces for textile treatment at neutral pH. In: Enzyme and Microbial Technology. 2004 ; Vol. 34, No. 3-4. pp. 332 - 341.
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abstract = "Culture supernatants from strains of Melanocarpus albomyces, Myceliophthora thermophila, Chaetomium thermophilum, and Sporotrichum thermophilum were tested for their ability to release dye in neutral pH conditions from indigo-dyed cotton-containing fabric in biostoning applications. The supernatants from M. albomyces worked well in biostoning, with low backstaining. Three cellulases were purified to homogeneity from the culture medium of this species. Two of the cellulases were endoglucanases with apparent molecular masses of 20 and 50 kDa. The 20 kDa endoglucanase was a relatively heat-stable cellulase with high pH optimum. The partially purified enzyme crystallized spontaneously at pH 4.0 and 7 °C. The 50 kDa endoglucanase also had activity against 4-methylumbelliferyl-β-d-lactoside (MUL) and was active over a wide range of pH values. The third purified cellulase was the 50 kDa cellobiohydrolase with low MUL activity at acidic pH and detectable activity towards filter paper and acid swollen Solca Floc-cellulose, but no endoglucanase activity. The purified 20 kDa endoglucanase performed well in biostoning of denim fabric at neutral pH. Addition of the purified 50 kDa endoglucanase or the 50 kDa cellobiohydrolase to the 20 kDa endoglucanase decreased backstaining in biostoning.",
keywords = "Melanocarpus albomyces, Textile treatment, Neutral cellulase",
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Miettinen-Oinonen, A, Londesborough, J, Joutsjoki, V, Lantto, R & Vehmaanperä, J 2004, 'Three cellulases from Melanocarpus albomyces for textile treatment at neutral pH', Enzyme and Microbial Technology, vol. 34, no. 3-4, pp. 332 - 341. https://doi.org/10.1016/j.enzmictec.2003.11.011

Three cellulases from Melanocarpus albomyces for textile treatment at neutral pH. / Miettinen-Oinonen, Arja (Corresponding Author); Londesborough, John; Joutsjoki, Vesa; Lantto, Raija; Vehmaanperä, Jari.

In: Enzyme and Microbial Technology, Vol. 34, No. 3-4, 2004, p. 332 - 341.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Three cellulases from Melanocarpus albomyces for textile treatment at neutral pH

AU - Miettinen-Oinonen, Arja

AU - Londesborough, John

AU - Joutsjoki, Vesa

AU - Lantto, Raija

AU - Vehmaanperä, Jari

PY - 2004

Y1 - 2004

N2 - Culture supernatants from strains of Melanocarpus albomyces, Myceliophthora thermophila, Chaetomium thermophilum, and Sporotrichum thermophilum were tested for their ability to release dye in neutral pH conditions from indigo-dyed cotton-containing fabric in biostoning applications. The supernatants from M. albomyces worked well in biostoning, with low backstaining. Three cellulases were purified to homogeneity from the culture medium of this species. Two of the cellulases were endoglucanases with apparent molecular masses of 20 and 50 kDa. The 20 kDa endoglucanase was a relatively heat-stable cellulase with high pH optimum. The partially purified enzyme crystallized spontaneously at pH 4.0 and 7 °C. The 50 kDa endoglucanase also had activity against 4-methylumbelliferyl-β-d-lactoside (MUL) and was active over a wide range of pH values. The third purified cellulase was the 50 kDa cellobiohydrolase with low MUL activity at acidic pH and detectable activity towards filter paper and acid swollen Solca Floc-cellulose, but no endoglucanase activity. The purified 20 kDa endoglucanase performed well in biostoning of denim fabric at neutral pH. Addition of the purified 50 kDa endoglucanase or the 50 kDa cellobiohydrolase to the 20 kDa endoglucanase decreased backstaining in biostoning.

AB - Culture supernatants from strains of Melanocarpus albomyces, Myceliophthora thermophila, Chaetomium thermophilum, and Sporotrichum thermophilum were tested for their ability to release dye in neutral pH conditions from indigo-dyed cotton-containing fabric in biostoning applications. The supernatants from M. albomyces worked well in biostoning, with low backstaining. Three cellulases were purified to homogeneity from the culture medium of this species. Two of the cellulases were endoglucanases with apparent molecular masses of 20 and 50 kDa. The 20 kDa endoglucanase was a relatively heat-stable cellulase with high pH optimum. The partially purified enzyme crystallized spontaneously at pH 4.0 and 7 °C. The 50 kDa endoglucanase also had activity against 4-methylumbelliferyl-β-d-lactoside (MUL) and was active over a wide range of pH values. The third purified cellulase was the 50 kDa cellobiohydrolase with low MUL activity at acidic pH and detectable activity towards filter paper and acid swollen Solca Floc-cellulose, but no endoglucanase activity. The purified 20 kDa endoglucanase performed well in biostoning of denim fabric at neutral pH. Addition of the purified 50 kDa endoglucanase or the 50 kDa cellobiohydrolase to the 20 kDa endoglucanase decreased backstaining in biostoning.

KW - Melanocarpus albomyces

KW - Textile treatment

KW - Neutral cellulase

U2 - 10.1016/j.enzmictec.2003.11.011

DO - 10.1016/j.enzmictec.2003.11.011

M3 - Article

VL - 34

SP - 332

EP - 341

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 3-4

ER -