Tissue culture and genetic engineering of an important anticancer compound producing plant Veratrum californicum Duran

Research output: Contribution to journalOther journal contributionScientific

Abstract

Veratrum californicum Duran (Liliaceae) is an important monocotyledonous medicinal plant which is the only source of the anticancer compound cyclopamine. The in vitro platform is needed for utilization of Veratrum cells in the production of the important secondary metabolites. Tissue culture, green plant regeneration and genetic engineering of V. californicum were developed. Fine suspension cell lines were established from germinated mature embryos by embloying friable embryogenic calli, AA- and L2-medium as culture media. The suspension cells were cryopreserved successfully and recovered at a high rate. Green plants were regenerated from embryogenic calli maintained on solid medium with 73% regeneration ability (green plants/100 calli) in 27 months old culture. The in vitro plantlets contained the steroid alkaloids cyclopamine and veratramine. In addition, Agrobacterium-mediated and protoplast-based transformation methods were developed. For the first time, the basic tools for the metabolic engineering and biotechnological production of secondary metabolites of V. californicum are now available.
Original languageEnglish
JournalPlanta Medica
Volume72
Issue number11
DOIs
Publication statusPublished - 2006
MoE publication typeB1 Article in a scientific magazine
Event54th Annual Congress on Medicinal Plant Research - Helsinki, Finland
Duration: 29 Aug 20062 Sep 2006

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Veratrum
Viridiplantae
Genetic engineering
Tissue culture
Genetic Engineering
Bony Callus
Tissue Engineering
Metabolites
Suspensions
Metabolic engineering
Regeneration
Alkaloids
Liliaceae
Metabolic Engineering
Culture Media
Agrobacterium
Protoplasts
Steroids
Cells
Medicinal Plants

Cite this

@article{e0a98bda2c1847588fb73af685d9e243,
title = "Tissue culture and genetic engineering of an important anticancer compound producing plant Veratrum californicum Duran",
abstract = "Veratrum californicum Duran (Liliaceae) is an important monocotyledonous medicinal plant which is the only source of the anticancer compound cyclopamine. The in vitro platform is needed for utilization of Veratrum cells in the production of the important secondary metabolites. Tissue culture, green plant regeneration and genetic engineering of V. californicum were developed. Fine suspension cell lines were established from germinated mature embryos by embloying friable embryogenic calli, AA- and L2-medium as culture media. The suspension cells were cryopreserved successfully and recovered at a high rate. Green plants were regenerated from embryogenic calli maintained on solid medium with 73{\%} regeneration ability (green plants/100 calli) in 27 months old culture. The in vitro plantlets contained the steroid alkaloids cyclopamine and veratramine. In addition, Agrobacterium-mediated and protoplast-based transformation methods were developed. For the first time, the basic tools for the metabolic engineering and biotechnological production of secondary metabolites of V. californicum are now available.",
author = "Anneli Ritala and Ruin Ma and Liisa Nohynek and Tapani Suortti and Heiko Rischer and Kirsi-Marja Oksman-Caldentey",
note = "Only abstract published",
year = "2006",
doi = "10.1055/s-2006-949789",
language = "English",
volume = "72",
journal = "Planta Medica",
issn = "0032-0943",
publisher = "Georg Thieme Verlag",
number = "11",

}

Tissue culture and genetic engineering of an important anticancer compound producing plant Veratrum californicum Duran. / Ritala, Anneli; Ma, Ruin; Nohynek, Liisa; Suortti, Tapani; Rischer, Heiko; Oksman-Caldentey, Kirsi-Marja.

In: Planta Medica, Vol. 72, No. 11, 2006.

Research output: Contribution to journalOther journal contributionScientific

TY - JOUR

T1 - Tissue culture and genetic engineering of an important anticancer compound producing plant Veratrum californicum Duran

AU - Ritala, Anneli

AU - Ma, Ruin

AU - Nohynek, Liisa

AU - Suortti, Tapani

AU - Rischer, Heiko

AU - Oksman-Caldentey, Kirsi-Marja

N1 - Only abstract published

PY - 2006

Y1 - 2006

N2 - Veratrum californicum Duran (Liliaceae) is an important monocotyledonous medicinal plant which is the only source of the anticancer compound cyclopamine. The in vitro platform is needed for utilization of Veratrum cells in the production of the important secondary metabolites. Tissue culture, green plant regeneration and genetic engineering of V. californicum were developed. Fine suspension cell lines were established from germinated mature embryos by embloying friable embryogenic calli, AA- and L2-medium as culture media. The suspension cells were cryopreserved successfully and recovered at a high rate. Green plants were regenerated from embryogenic calli maintained on solid medium with 73% regeneration ability (green plants/100 calli) in 27 months old culture. The in vitro plantlets contained the steroid alkaloids cyclopamine and veratramine. In addition, Agrobacterium-mediated and protoplast-based transformation methods were developed. For the first time, the basic tools for the metabolic engineering and biotechnological production of secondary metabolites of V. californicum are now available.

AB - Veratrum californicum Duran (Liliaceae) is an important monocotyledonous medicinal plant which is the only source of the anticancer compound cyclopamine. The in vitro platform is needed for utilization of Veratrum cells in the production of the important secondary metabolites. Tissue culture, green plant regeneration and genetic engineering of V. californicum were developed. Fine suspension cell lines were established from germinated mature embryos by embloying friable embryogenic calli, AA- and L2-medium as culture media. The suspension cells were cryopreserved successfully and recovered at a high rate. Green plants were regenerated from embryogenic calli maintained on solid medium with 73% regeneration ability (green plants/100 calli) in 27 months old culture. The in vitro plantlets contained the steroid alkaloids cyclopamine and veratramine. In addition, Agrobacterium-mediated and protoplast-based transformation methods were developed. For the first time, the basic tools for the metabolic engineering and biotechnological production of secondary metabolites of V. californicum are now available.

U2 - 10.1055/s-2006-949789

DO - 10.1055/s-2006-949789

M3 - Other journal contribution

VL - 72

JO - Planta Medica

JF - Planta Medica

SN - 0032-0943

IS - 11

ER -